光谱学与光谱分析 |
|
|
|
|
|
Raman Analysis of Conformation Changes of Insulin Solvent after Being Exposed to ELF Pulsed Electric Field |
TIAN Bing1, 2, JIA Cai-li1, XIA Ruo-hong1, CHEN Shu-de1 |
1. Key Laboratory for Optical and Magnetic Resonance Spectroscopy, Department of Physics, East China Normal University, Shanghai 200062, China 2. Department of Physics, Liaocheng University, Liaocheng 252059, China |
|
|
Abstract Raman spectra of insulin solvents are presented before and after being exposed to the pulsed electric field with extremely low frequency 50 Hz(ELF). The covalences of the molecule were not affected and the changes of some secondary bonds such as hydrogen bonds and salt bonds were observed. Detailed analysis of these spectra indicates that the α-helix of insulin molecule was destroyed after the exposure, which is proved by the shift of the peak of the amide Ⅰ region toward higher wave number and by the appearance of several new peakss: 1 561 and 1 594 cm-1 . Uhe disulfides were affected by the weaken α-helix, and their vibrational moees were changed. Meanwhile the hydrogen bonding between the dimer are broken down which leads to the increase in the peak intensities at 1 002 and at 1 602 cm-1.
|
Received: 2003-02-08
Accepted: 2003-08-06
|
|
Corresponding Authors:
TIAN Bing
|
|
Cite this article: |
TIAN Bing,JIA Cai-li,XIA Ruo-hong, et al. Raman Analysis of Conformation Changes of Insulin Solvent after Being Exposed to ELF Pulsed Electric Field [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2004, 24(11): 1331-1333.
|
|
|
|
URL: |
http://www.gpxygpfx.com/EN/Y2004/V24/I11/1331 |
[1] 孙大业,郭艳林等. 细胞信号转导. 北京:科学出版社,2001. [2] Brandenburg Dietrich, Wollmer Axel. Insulin Chemistry, Structure and Function of Insulin and Related Hormones, in Proceeding of Second International Insulin Symposium Aachen Germany, 1980. [3] Reipa V, Gsigalas A, Abramowitz S. J. Electroanal. Chem., 1993, 348: 413. [4] CHENG Ji-ji.(程极济). Opitic Biophysics(光生物物理学). Beijing:Higher Education Press(北京:高等教育出版社),1987. [5] LI Xiang, CHEN Shu-de, WANG Li-ying, ZHANG Hong-feng, YE Shi-jing(李 祥,陈树德,王丽英, 张红锋,叶士景). Spectroscopy and Spectral Analysis(光谱学与光谱分析),2001,21(3):406. [6] Yu Nai-Teng, Liu C S. J. Mol. Biol., 1972, 70: 117. [7] Venyaminov S Yu, Kalnin N N. Biopolymers, 1990, 30(13-14): 1243. [8] Isaish pittman I V. Biochemistry, 1995, 34: 10578. [9] DING Li-ping, ZHANG Hong-feng et al(丁荔萍,张红锋等). Chinese Journal of Cell Biology(细胞生物学杂志),2002,24(5):303.
|
[1] |
WANG Gan-lin1, LIU Qian1, LI Ding-ming1, YANG Su-liang1*, TIAN Guo-xin1, 2*. Quantitative Analysis of NO-3,SO2-4,ClO-4 With Water as Internal Standard by Raman Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(06): 1855-1861. |
[2] |
HUANG Bin, DU Gong-zhi, HOU Hua-yi*, HUANG Wen-juan, CHEN Xiang-bai*. Raman Spectroscopy Study of Reduced Nicotinamide Adenine Dinucleotide[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(06): 1679-1683. |
[3] |
ZHU Xiang1, 2*, YUAN Chao-sheng1, CHENG Xue-rui1, LI Tao1, ZHOU Song1, ZHANG Xin1, DONG Xing-bang1, LIANG Yong-fu2, WANG Zheng2. Study on Performances of Transmitting Pressure and Measuring Pressure of [C4mim][BF4] by Using Spectroscopic Techniques[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(06): 1674-1678. |
[4] |
WANG Ming-xuan, WANG Qiao-yun*, PIAN Fei-fei, SHAN Peng, LI Zhi-gang, MA Zhen-he. Quantitative Analysis of Diabetic Blood Raman Spectroscopy Based on XGBoost[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(06): 1721-1727. |
[5] |
YOU Gui-mei1, ZHANG Wen-jie1, CAO Zhen-wei2, HAN Xiang-na1*, GUO Hong1. Analysis of Pigments of Colored Paintings From Early Qing-Dynasty Fengxian Dian in the Forbidden City[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(06): 1874-1880. |
[6] |
LI Qing1, 2, XU Li1, 2, PENG Shan-gui1, 2, LUO Xiao1, 2, ZHANG Rong-qin1, 2, YAN Zhu-yun3, WEN Yong-sheng1, 2*. Research on Identification of Danshen Origin Based on Micro-Focused
Raman Spectroscopy Technology[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(06): 1774-1780. |
[7] |
WANG Zhong, WAN Dong-dong, SHAN Chuang, LI Yue-e, ZHOU Qing-guo*. A Denoising Method Based on Back Propagation Neural Network for
Raman Spectrum[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(05): 1553-1560. |
[8] |
FU Qiu-yue1, FANG Xiang-lin1, ZHAO Yi2, QIU Xun1, WANG Peng1, LI Shao-xin1*. Research Progress of Pathogenic Bacteria and Their Drug Resistance
Detection Based on Surface Enhanced Raman Technology[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(05): 1339-1345. |
[9] |
YAN Ling-tong, LI Li, SUN He-yang, XU Qing, FENG Song-lin*. Spectrometric Investigation of Structure Hydroxyl in Traditional Ceramics[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(05): 1361-1365. |
[10] |
ZHAO Yong1, HE Men-yuan1, WANG Bo-lin2, ZHAO Rong2, MENG Zong1*. Classification of Mycoplasma Pneumoniae Strains Based on
One-Dimensional Convolutional Neural Network and
Raman Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(05): 1439-1444. |
[11] |
LI Meng-meng1, TENG Ya-jun2, TAN Hong-lin1, ZU En-dong1*. Study on Freshwater Cultured White Pearls From Anhui Province Based on Chromaticity and Raman Spectra[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(05): 1504-1507. |
[12] |
JIAO Ruo-nan, LIU Kun*, KONG Fan-yi, WANG Ting, HAN Xue, LI Yong-jiang, SUN Chang-sen. Research on Coherent Anti-Stokes Raman Spectroscopy Detection of
Microplastics in Seawater and Sand[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(04): 1022-1027. |
[13] |
ZHANG Li-sheng. Photocatalytic Properties Based on Graphene Substrate[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(04): 1058-1063. |
[14] |
LÜ Yang, PEI Jing-cheng*, GAO Ya-ting, CHEN Bo-yu. Chemical Constituents and Spectra Characterization of Gem-Grade
Triplite[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(04): 1204-1208. |
[15] |
FU Ying-ying, ZHANG Ping, ZHENG Da-wei , LIN Tai-feng*, WANG Hui-qin, WU Xi-hao, SONG Jia-chen. Preparation and SERS Performance of Au-Nylon Flexible Membrane Substrate[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(03): 692-698. |
|
|
|
|