Resonance Light Scattering Study on the Interaction of Victory Blue-B with Deoｘyribonucleic Acid and Application to DNA Assay
SU Jie-shu1，2，CHEN Xiao-ming1，LONG Yun-fei2，LI Wei2，LUO Hai-tang2
1. Institute of Chemistry, Xiangtan University, Xiangtan 411105，China 2. Department of Chemistry, Hu'nan University of Science and Technology, Xiangtan 411201, China
Abstract：The enhancement of the Resonance Light Scattering (RLS) due to Victory blue-B on the molecular surface of DNA in alkalescence solution was studied. A method of resonance light scattering was developed to determine DNA. Deoxyribonucleic acid(DNA) was studied in the range of pH 9.0-10.5. The effects of interference substances and optimization of procedure were investigated and a new method was established for the determination of DNA at nanogram level based on RLS measurement. The linear range of the DNA is 0-3.0 mg·L-1 with a correlation coefficient of 0.997 8. The detection limit is 21.5 μg·L-1. The method has been applied to the determination of DNA in mixed samples with satisfactory result.
苏界殊1，2，陈小明1*，龙云飞2，李 维2，罗海棠2 . 维多利亚蓝B与DNA作用的共振光谱特性及分析应用 [J]. 光谱学与光谱分析, 2004, 24(01): 37-40.
SU Jie-shu1，2，CHEN Xiao-ming1，LONG Yun-fei2，LI Wei2，LUO Hai-tang2 . Resonance Light Scattering Study on the Interaction of Victory Blue-B with Deoｘyribonucleic Acid and Application to DNA Assay . SPECTROSCOPY AND SPECTRAL ANALYSIS, 2004, 24(01): 37-40.
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