Abstract:The interaction between thionine and deoxyribonucleic acid was studied with ethidium bromide probe in Tris-HCl buffer solution at pH 7.4 by fluorescence spectra, electronic absorption spectra, and resonance light scattering spectra. From the obtained results, it was found obviously that the planar structure of thionine can be intercalated into the stacked base pairs of DNA, which is the major controlling factor. The binding constant was found to be 1.0×105 L·mol-1.
刘雪平,冯素玲*, 潘自红,樊静. 溴化乙锭探针研究劳氏青莲与脱氧核糖核酸的相互作用[J]. 光谱学与光谱分析, 2006, 26(10): 1895-1898.
LIU Xue-ping,FENG Su-ling*, PAN Zi-hong, FAN Jing . Studies on the Interaction between Thionine and Deoxyribo-Nucleic Acid by Ethidium Bromide Probe. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2006, 26(10): 1895-1898.
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