Abstract:In pH 4.0 acetic acid-sodium acetate buffer solution, cationic dye victory blue B shows an absorption peak at 614 nm, and the absorption peak decreases after it reacts with chondroitin sulfate to form association particles. The decrease in absorption value is linear with chondroitin sulfate concentration in the range of 0.1-5 μg·mL-1, and the correlation efficient is 0.998 6. The method was applied to the determination of chondroitin sulfate in synthesis samples and real samples with rapidity, simplicity and good accuracy.
Key words:Chondroition sulfate;Victory blue B;Association particle;Spectrophotometry
[1] XI Jun, ZHANG You-sen(奚 俊,张有森). Biological J.(生物学杂志), 2000, 17(6):33. [2] QIN Zhen-shun, CHANG Feng-qi, HAN Hui-xin, et al(秦振顺,常凤启,韩会新,等). Chin. J. Health Laboratory Technology(中国卫生检验杂志), 2002, 12(3):306. [3] Du J P,Ddington N E. Anal. Biochem., 2002, 306:252. [4] SHI Wen-jian, WU Qiu-hua, ZHUANG Qi-jia(施文健,吴秋华,庄奇佳). Phys. Testing and Chem. Anal. Part B: Chem. Anal(理化检验-化学分册),2003,39(3):147. [5] GAO Gui-zhen, JIAO Qing-cai, DING Yi-lei, et al(高贵珍,焦庆才,丁一磊,等). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2003, 23(3):600. [6] XU Hong, LIU Shao-pu, LUO Hong-qun(徐 红,刘绍璞,罗红群),Phys. Testing and Chem. Anal. Part B: Chem. Anal(理化检验-化学分册),2002,38(5):217. [7] LUO Yang-he, JIANG Zhi-liang(罗杨合,蒋治良). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2004, 24(12):1630. [8] LIANG Ai-hui, JIANG Zhi-liang, LI Zhen-zhong, et al(梁爱惠, 蒋治良, 李振中, 等). Chin. J. Appl. Chem.(应用化学),2004,21:1217.