子宫病变组织的拉曼光谱研究

doi:10.3964/j.issn.1000-0593(2008)10-2338-05

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Abstract The Raman spectrum can reflect the differences in chemical components and molecular structures of tissues and cells, and significant progress has been made in the research on structures, functions and diseases of cells and tissues with Raman spectroscopy. A fiber Raman spectrometer was used to measure the Raman spectra of some uterine malignant, benign, and normal tissues, such as uterine myometrium tissue, uterine myoma tissue, normal endometrium tissue, malignant endometrium tissue and adenomyosis tissue. After having compared the Raman spectrum of pathological tissues with that of the corresponding normal tissues, we observed that the peak referring to Methionine ν(C—S) (Met ν(C—S)) splits into two peaks in the uterine myoma tissues caused by the vibrations of tryptophan (Trp) and cartotene, which are not present in the normal tissues. There is a peak at 1 447 cm^-1 in the endometrium tissues corresponding to CH₂—CH₃ def, which is one of the characteristic peaks of cancerous tissues. For the adenomyosis tissues, a peak caused by νC—C) skeletal-α helix is obviously weaker than that in normal tissue, and the peak induced by δ(C—O) shifts from 1 160 cm-1 in normal tissues to 1 173 cm^-1 in the adenomyosis tissues. Thus, it was demonstrated that the technology of Raman spectroscopy is available for distinguishing different pathological uterine tissues at molecular level. This study is not only helpful on early diagnosis of uterine diseases, but also very crucial for the basic research on uterine diseases. And the Raman spectroscopy technology based on optic-fibers has a potential to evolve into a highly sensitive technology for diagnosis.

Key words：Raman spectrum;Uterine myoma tissue;Endometrium cancer;Adenomyosis tissue

Received: 2007-06-19 Accepted: 2007-09-18

ZTFLH:

O657.3

Corresponding Authors: ZHU Dan

Cite this article:

WANG He-qun,Lü Gang,SHI Zhen-zhi, et al. Study on the Pathological Uterine Tissues with a Fiber Raman Spectrometry[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2008, 28(10): 2338-2342.

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https://www.gpxygpfx.com/EN/10.3964/j.issn.1000-0593(2008)10-2338-05 OR https://www.gpxygpfx.com/EN/Y2008/V28/I10/2338

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