A SERS-Aptsensor for Detection of Chloramphenicol Based on DNA Hybridization Indicator and Silver Nanorod Array Chip
GUO He-yuanxi1, LI Li-jun1*, FENG Jun1, 2*, LIN Xin1, LI Rui1
1. Guangxi Key Laboratory of Green Processing of Sugar Resources, School of Biological and Chemical Engineering, Guangxi University of Science and Technology,Liuzhou 545006, China
2. Faculty of Medicine,Guangxi University of Science and Technology, Liuzhou 545005, China
Abstract Chloramphenicol (CAP) is a synthetic antibiotic that inhibits protein synthesis by binding to the ribosomes of bacteria to achieve the purpose of antibacterial. Long-term intake of residual CAP animal-derived food can lead to anemia and leukemia in the human body and can also cause the body to develop drug resistance, which will seriously endanger human health. Many countries have regulations prohibiting the detection of CAP in livestock products. Therefore, designing a more rapid, simple and highly specific CAP detection method is of great significance. In this paper, the thiolated aptamer of CAP (SH-Apt) was used to modify the silver nanorod array chip(chip)as the SERS substrate, and the DNA hybridization indicator methylene blue (MB) as the Raman reporter, A novel high-specificity CAP-SERS aptamer sensor was constructed by utilizing the competitive binding relationship between CAP, CAP aptamer complementary strand (cDNA) and CAP aptamer (Apt). The chip and CAP-SERS aptamer sensor were characterized by scanning electron microscopy (SEM), X-ray diffraction (XRD) and EDS spectroscopy. The results showed that a large amount of silver elements were uniformly distributed on the surface of the chip and the CAP-SERS aptamer sensor was successfully prepared. Detection of CAP standards at room temperature, the results of the sensor-related performance analysis suggest that, with an increase of CAP concentration (0.001~10 ng·mL-1) caused the decreased SERS signal at 1 624 cm-1(ISERS) intensity (ISERS=-971logc+1 983). A good negative correlation (R2=0.991) was achieved, and the limit of detection (LOD) was as low as 0.2 pg·mL-1 (S/N=3), the Raman enhancement factor EF=1.01×107. Further indicating that the substrate has good Raman enhancement. The sensor is used to detect individual CAP tablets and CAP in human and pig sera, spik experiments were performed, and the results were satisfactory. The recovery and relative standard deviations(RSD) were 91.2%~120.5% and 0.97%~8.1%, which proved that the sensor had good accuracy. The sensor is intended to be used for the rapid quantitative detection of CAP in real samples because of its simple manufacture, high sensitivity, strong selectivity, good reproducibility, good stability, and fast detection speed. It provides a new idea for detecting CAP.
Corresponding Authors:
LI Li-jun, FENG Jun
E-mail: lilijun0562@sina.com;hxpfengjun@163.com
Cite this article:
GUO He-yuanxi,LI Li-jun,FENG Jun, et al. A SERS-Aptsensor for Detection of Chloramphenicol Based on DNA Hybridization Indicator and Silver Nanorod Array Chip[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(11): 3445-3451.
[1] Li Y B, Wang L, Zhao L T, et al. Food Chemistry, 2021, 357: 129769.
[2] Chen Y, Chen L, Ou Y D, et al. Sensors and Actuators B: Chemical, 2016, 233: 691.
[3] Wu S J, Zhang H, Zhao S, et al. Food Control, 2015, 50: 597.
[4] Mehta J, Van Dorst B, Rouah-Martin E, et al. Journal of Biotechnology, 2011, 155(4): 361.
[5] Chen H X, Chen H, Ying J, et al. Analytica Chimica Acta, 2009, 632(1): 80.
[6] Li P, Qiu Y M, Cai H X, et al. Chinese Journal of Chromatography, 2006, 4(1): 14.
[7] Huang J F, Zhang H J, Feng Y Q. Journal of Agricultural & Food Chemistry, 2006, 54(25): 9279.
[8] Weng S Z, Hu X J, Zhu W X, et al. Food Chemistry, 2021, 359: 129847.
[9] Tálas E, Szöllösi G, Kristyan S, et al. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 2021, 260: 119912.
[10] CHEN Shi, WU Jing, WANG Chao-nan, et al(陈 实, 吴 静, 王超男, 等). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2020, 40(3): 765.
[11] Yao Y Y, Ji J, Zhang H D, et al. Analytical Chemistry, 2018, 90(18): 10394.
[12] Vannucci G, Canamares M V, Prati S, et al. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 2021, 261(Sup1): 120007.
[13] Jiang L, Hassan Md M, Jiao T H, et al. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 2021, 261: 119996.
[14] Chen Q, Qin L X, Shi C L, et al. Chemosphere, 2021, 282: 131000.
[15] Zhang M L, Li X D, Pan J L, et al. Biosensors and Bioelectronics, 2021, 190: 113421.
[16] Huo Chengcheng, Han Wanying, Tang Wei, et al. Talanta, 2021, 234: 122678.
[17] DONG Ya-fei, HU Wen-xiao, QIAN Meng-yao, et al(董亚非, 胡文晓, 钱梦瑶, 等). Journal of Electronics & Information(电子与信息学报), 2020, 42(6): 1374.
[18] XU Shi-chao, ZHANG Ji-mei, YAO Cui-cui, et al(许世超, 张纪梅, 姚翠翠, 等). Fine Chemicals(精细化工), 2008, 25(12): 1183.
[19] HUANG Lu-mao, DU Pei-yan, CHEN Lan, et al(黄陆茂,杜佩妍,陈 蓝,等). Journal of Southern Medical University(南方医科大学学报), 2018, 38(4): 414.
[20] Mo F, Han M, Weng X, et al. Analytical Chemistry, 2021, 93(3): 1764.
[21] Farjami Elaheh, Campos Rui, Ferapontova Elena E. Langmuir, 2016, 32(3): 928.
[22] Zhang Z X, Wang X L, Wang Y, et al. Analyst, 2010, 135(11): 2960.
[23] Zheng J Y, Feng R R, He C M, et al. Chemical Research in Chinese Universities, 2018, 34(6): 952.
[24] XU Lian-ying, PENG Hai-xia, SHAO Yu-yu, et al(徐连应, 彭海霞, 邵玉宇, 等). Scientia Agricultura Sinica(中国农业科学), 2018, 51(6): 1192.
[25] Yu Z H, Luan Y N, Li H Y, et al. Sensors and Actuators B: Chemical, 2019, 284: 73.
[26] Tesema T E, Kafle B, Tadesse M G, et al. The Journal of Physical Chemistry C, 2017, 121(13): 7421.
[27] Ashurov M, Abdusatorov B, Baranchikov A, et al. Physical Chemistry Chemical Physics, 2021, 23(36): 20275.
[28] HU Yong-hong, RONG Jian-hua, LIU Ying-liang, et al(胡永红,容建华,刘应亮,等). Acta Chimica Sinica(化学学报), 2005, 24: 2189.
[29] Yu Lei, Du Peng, Hu Jia, et al. Journal of Materials Science: Materials in Elctronics, 2021, 32(1): 1.
[30] Huang Z P, Zhang R, Chen H, et al. Biosensors and Bioelectronics, 2019, 142: 111542.
[31] WU Hai-xia, KANG Jing-wan, LI Zhi-feng, et al(吴海霞, 康敬万, 李志峰, 等). Journal of Instrumental Analysis(分析测试学报), 2006, 25(4): 1.
[32] GAN Yi-ting, CHEN Lin-lin, ZHU Yao-ting, et al(甘绮婷,陈琳琳,朱耀婷,等). Journal of South China Normal University(Natural Science Edition)[华南师范大学学报(自然科学版)], 2017, 49(2): 1.