| 光谱学与光谱分析 |
|
|
|
|
|
| Raman Spectroscopic Study of Human Tissues |
| LIU Gang1, LIU Jian-hong2, ZHANG Lin3, YU Fan4, SUN Shi-zhong2 |
1. Department of Physics, Yunnan Normal University, Kunming 650092，China
2. Center of Analysis and Testing, Yunnan Normal University, Kunming 650092, China
3. Department of Pathology, The First Hospital of Kunming Medical College, Kunming 650031，China
4. Center of Experiment, Yunnan University, Kunming 650091, China |
|
|
|
|
Abstract Raman spectroscopy has been applied to the investigation of human thyroid, lung, oviduct, ovarian, cervical, and uterine tissues. Thyroid and uterine tissues exhibit more Raman bands than the other tissues in the range from 500 to 3 500 cm-1 with 514.5 nm excitation, while lung and cervical tissue do not show any bands. In the range of 600-1 800 cm-1, uterine and ovarian tissues show only the Raman features of carotenoids near 1 004, 1 158, and 1 520 cm-1. Remarkable differences between normal and follicular carcinoma samples of thyroid were observed by the absence of characteristic bands at 1 585 and 1 634 cm-1 in the latter. The spectral intensity of lung cancerous sample is much lower than that of normal sample, by which normal and malignant samples can be discriminated. The results indicate that Raman spectroscopic technique will play an important rule in clinical diagnosing.
|
|
Received: 2003-12-08
Accepted: 2004-03-28
|
|
|
|
Corresponding Authors:
LIU Gang
|
|
[1] Manoharan R, Wang Y, Feld M S. Spectrochimica Acta Part A, 1996, 52: 215.
[2] Ci Y X, Gao T Y, Feng J, Guo Z Q. Appl. Spectrosc., 1999, 53: 312.
[3] Rigas B, Morgello S, Goldman I S，Wong P T T. Proc. Natl. Acad. Sci. USA, 1990, 87: 8140.
[4] Chiriboga L, Yee H, Diem M. Appl. Spectrosc., 2000, 54: 1.
[5] Wong P T T, Wong R K, Caputo T A et al. Proc. Natl. Acad. Sci. USA, 1991, 88: 10988.
[6] Lawson E E, Barry B W, Williams D, Edwards H G M. J. Raman Spectrosc., 1997, 28: 111.
[7] Boustany N N, Manoharan R, Dasari R R, Feld M S. Appl. Spectrosc., 2000, 54: 24.
[8] Utzinger U, Heintzelman D L, Mahadevan-Jansen A et al. Appl. Spectrosc., 2001, 55: 955.
[9] Yazdi Y, Ramanujam N, Lotan R et al. Appl. Spectrosc.，1999, 53: 82.
[10] Mahadevan-Jensen A, Mitchell M F, Ramnaujam N et al. Photochem. Photobiol., 1998, 68: 123.
[11] Hanlon E B, Manoharan R, Koo T W et al. Phys. Med. Biol., 2000, 45: R1.
[12] Choo Smith L P, Edwards H G M, Endtz H P et al. Biopolymers(Biospectroscopy), 2002, 67: 1.
[13] Redd D C B, Feng Z C, Yue K T, Gansler T S. Appl. Spectrosc., 1993, 47: 787.
[14] Frank C J, Redd D C B, Gansler T S, McCreery R L. Anal. Chem., 1994, 66: 319.
[15] Frank C J, McCreery R L, Redd D C B, Gansler T S. Appl. Spectrosc., 1993, 47: 387.
[16] Schaeberie M D, Kalasinsky V F, Luke J L et al. Anal. Chem., 1996, 68: 1829.
[17] YAN Xun-ling, DONG Rui-xin, WANG Qiu-guo, CHEN Shuang-feng, ZHANG Zong-wang et al(闫循领，董瑞新，王秋国，陈双峰，张宗旺等). Spectroscopy and Spectral Analysis(光谱学与光谱分析)，2005，25(1)：58.
[18] Parker S F, Tavender S M, Dixon N M et al. Appl. Spectrosc., 1999, 53: 86.
[19] WANG Jia-yao, MA Jin-long(王家耀，马金龙). Clinical Pathology of the Thyroid and Parathyroid(甲状腺与甲状旁腺临床病理学). Jinan：Shandong Science and Technology Press(济南：山东科学技术出版社)，2000. 1.
|
| [1] |
LI Jie, ZHOU Qu*, JIA Lu-fen, CUI Xiao-sen. Comparative Study on Detection Methods of Furfural in Transformer Oil Based on IR and Raman Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(01): 125-133. |
| [2] |
WANG Fang-yuan1, 2, HAN Sen1, 2, YE Song1, 2, YIN Shan1, 2, LI Shu1, 2, WANG Xin-qiang1, 2*. A DFT Method to Study the Structure and Raman Spectra of Lignin
Monomer and Dimer[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(01): 76-81. |
| [3] |
XING Hai-bo1, ZHENG Bo-wen1, LI Xin-yue1, HUANG Bo-tao2, XIANG Xiao2, HU Xiao-jun1*. Colorimetric and SERS Dual-Channel Sensing Detection of Pyrene in
Water[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(01): 95-102. |
| [4] |
WANG Xin-qiang1, 3, CHU Pei-zhu1, 3, XIONG Wei2, 4, YE Song1, 3, GAN Yong-ying1, 3, ZHANG Wen-tao1, 3, LI Shu1, 3, WANG Fang-yuan1, 3*. Study on Monomer Simulation of Cellulose Raman Spectrum[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(01): 164-168. |
| [5] |
WANG Lan-hua1, 2, CHEN Yi-lin1*, FU Xue-hai1, JIAN Kuo3, YANG Tian-yu1, 2, ZHANG Bo1, 4, HONG Yong1, WANG Wen-feng1. Comparative Study on Maceral Composition and Raman Spectroscopy of Jet From Fushun City, Liaoning Province and Jimsar County, Xinjiang Province[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(01): 292-300. |
| [6] |
LI Wei1, TAN Feng2*, ZHANG Wei1, GAO Lu-si3, LI Jin-shan4. Application of Improved Random Frog Algorithm in Fast Identification of Soybean Varieties[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(12): 3763-3769. |
| [7] |
WANG Zhi-qiang1, CHENG Yan-xin1, ZHANG Rui-ting1, MA Lin1, GAO Peng1, LIN Ke1, 2*. Rapid Detection and Analysis of Chinese Liquor Quality by Raman
Spectroscopy Combined With Fluorescence Background[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(12): 3770-3774. |
| [8] |
LIU Hao-dong1, 2, JIANG Xi-quan1, 2, NIU Hao1, 2, LIU Yu-bo1, LI Hui2, LIU Yuan2, Wei Zhang2, LI Lu-yan1, CHEN Ting1，ZHAO Yan-jie1*，NI Jia-sheng2*. Quantitative Analysis of Ethanol Based on Laser Raman Spectroscopy Normalization Method[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(12): 3820-3825. |
| [9] |
LU Wen-jing, FANG Ya-ping, LIN Tai-feng, WANG Hui-qin, ZHENG Da-wei, ZHANG Ping*. Rapid Identification of the Raman Phenotypes of Breast Cancer Cell
Derived Exosomes and the Relationship With Maternal Cells[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(12): 3840-3846. |
| [10] |
LI Qi-chen1, 2, LI Min-zan1, 2*, YANG Wei2, 3, SUN Hong2, 3, ZHANG Yao1, 3. Quantitative Analysis of Water-Soluble Phosphorous Based on Raman
Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(12): 3871-3876. |
| [11] |
GUO He-yuanxi1, LI Li-jun1*, FENG Jun1, 2*, LIN Xin1, LI Rui1. A SERS-Aptsensor for Detection of Chloramphenicol Based on DNA Hybridization Indicator and Silver Nanorod Array Chip[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(11): 3445-3451. |
| [12] |
ZHU Hua-dong1, 2, 3, ZHANG Si-qi1, 2, 3, TANG Chun-jie1, 2, 3. Research and Application of On-Line Analysis of CO2 and H2S in Natural Gas Feed Gas by Laser Raman Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(11): 3551-3558. |
| [13] |
LIU Jia-ru1, SHEN Gui-yun2, HE Jian-bin2, GUO Hong1*. Research on Materials and Technology of Pingyuan Princess Tomb of Liao Dynasty[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(11): 3469-3474. |
| [14] |
LI Wen-wen1, 2, LONG Chang-jiang1, 2, 4*, LI Shan-jun1, 2, 3, 4, CHEN Hong1, 2, 4. Detection of Mixed Pesticide Residues of Prochloraz and Imazalil in
Citrus Epidermis by Surface Enhanced Raman Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(10): 3052-3058. |
| [15] |
ZHAO Ling-yi1, 2, YANG Xi3, WEI Yi4, YANG Rui-qin1, 2*, ZHAO Qian4, ZHANG Hong-wen4, CAI Wei-ping4. SERS Detection and Efficient Identification of Heroin and Its Metabolites Based on Au/SiO2 Composite Nanosphere Array[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(10): 3150-3157. |
|
|
|
|
