葡萄蒸馏酒中氨基甲酸乙酯的SERS检测研究

doi:10.3964/j.issn.1000-0593(2024)09-2467-09

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Abstract Ethyl carbamate，A group 2A carcinogen, is commonly found in fermented foods such as grape, soy sauce, and pickles. Due to its toxicity and carcinogenicity, it can accumulate in organisms and produce a variety of lesions and has become a potential threat to the modern food processing industry. The traditional detection method of ethyl carbamate has the disadvantages of a complicated sample pretreatment process, long analysis time, expensive equipment, high requirements for testers, and the inability to detect large samples quickly. Therefore, there is an urgent need to develop a rapid, accurate, and efficient method for urethane detection. Surface-enhanced Raman spectroscopy (SERS) technology has the characteristics of high sensitivity, high selectivity, resistant photo bleaching, short response time, and non-destructive. SERS overcomes the problems of Raman spectroscopy, such as weak scattering signals and large background interference, and can realize the rapid qualitative and quantitative detection and analysis of trace substances, showing good application prospects in the territory of food safety detection. In this study, surface-enhanced Raman spectroscopy was used to detect the content of ethyl carbamate in grape spirit rapidly. Silver nanosphere silicon cone array and gold-silver alloy nanosphere silicon cone array were used as SERS substrates. The effects of SERS substrate structure, mixing time, storage time, and excitation wavelength on Raman scattering signals were investigated, and the optimal SERS substrate was determined. The results show that good Raman signals can be obtained when the excitation wavelength is 785 nm, the mixing time is 60 min, and the thickness of silver nanoparticles is 10 nm using Ag-Au/SiNCA as the SERS-enhanced substrate. The characteristic peak position of 1 442 cm^-1for quantitative analysis of ethyl carbamate in grape spirit was determined using density functional theory, and a linear equation was established between the peak intensity of the characteristic peak at 1 442 cm^-1and the EC concentration. The equation has a good linear relationship in the concentration range of 1×10^-3～1×10^-8 mol·L^-1, and the coefficient of determination R²=0.821 3. The recovery rate of the method is between 83.06% and 110.00%, and the minimum detection limit can reach 3.28×10^-8 mol·L^-1. This detection method is very simple and quick to operate, and analyze. If this method applies EC in grape spirit, an effective and rapid detection method can be provided with ideas and references.

Key words：Ethyl carbamate; Grape spirit; Surface-enhanced Raman spectroscopy

Received: 2023-06-26 Accepted: 2024-01-02

ZTFLH:

O657.3

Corresponding Authors: SUN Jing-tao E-mail: sunjingtaovv@126.com

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https://www.gpxygpfx.com/EN/10.3964/j.issn.1000-0593(2024)09-2467-09 OR https://www.gpxygpfx.com/EN/Y2024/V44/I09/2467

[1] LIU Tao-tao，CAI Kai，XIONG Xiao-tong，et al（刘涛涛，蔡凯，熊晓通, 等）. China Brewing（中国酿造），2023，42(10)：94.
[2] XIN Qian，JIA Shao-jie，ZHENG Xin-xin，et al（辛茜，贾少杰，郑欣欣，等）. Liquor-Making Science & Technology，2022，(1): 42.
[3] RAN Shi-li，WANG Hong-an，ZHANG Jie，et al（阮仕立，王宏安，张杰，等）. China Brewing（中国酿造），2022, (4): 45.
[4] LIANG Xin，ZHANG Cheng-nan，LI Xiu-ting（梁鑫，张成南，李秀婷）. China Brewing（中国酿造），2023，42(7)：1.
[5] SUN Jie-min，NING Huai-mei，ZHANG Cui-ge，et al（孙洁敏，宁淮梅，张翠格，等）. Food Science（食品科学），2015，36(18)：193.
[6] Li R，Zhang C C，Wang D，et al. Chinese Chemical Letters，2021，12(3)：76.
[7] Faried M，Ando S，Suga K，et al. Chemistry Letters，2020，106(9): 385.
[8] Zhao X，Jiang C. Food Chem.，2015，177：66.
[9] Du J, Wang H, Wang H, et al. Journal of Molecular Structure, 2017, 7: 35.
[10] Li M L，Zhao Y，Cui M L，et al. Analytical Sciences，2016，149：159.
[11] Qi Haiyan，Chen Huacai，Wang Yan，et al. Royal Society Open Science，2018，5(12)：42.
[12] Fu Hao，Bao Haoming，Zhang Hongwen，et al. ACS Omega，2021，6：18928.
[13] Yang Danting，Nicoleta E Mircescu，Zhou Haibo，et al. Journal of Raman Spectroscopy，2013，44(11)：1491.
[14] Ai Y J，Liang P，Wu Y X，et al. Food Chemistry，2018，241: 427.
[15] Li M，Zhao Y，Cui M，et al. Anal. Sci.，2016，32(7)：725.
[16] Chen M，Luo W，Zhang Z，et al. Talanta，2017，171：152.
[17] YANG De-hong，ZHANG Lei-lei，ZHU Cheng（杨德红，张雷蕾，朱诚）. Spectroscopy and Spectral Analysis（光谱学与光谱分析），2020，40(10)：3048.
[18] Polubotko A，Chelibanov V. Optics and Spectroscopy，2015，119(4)：664.
[19] Haoran F，Jinpeng W，Qingran M，et al. Molecules，2017，22(2)：1.
[20] Wu D，Li X，Lu J，et al. FEMS Microbiol. Lett.，2016，363(1)：1.
[21] Farrokhpour H, Ghandehari M. The Journal of Physical Chemistry C, 2019, 123(26)：153.
[22] Li C, Zhang C, Xu S, et al. Sensors & Actuators B: Chemical, 2018, 263(15): 164.
[23] Danting Y, Haibo Z, Yibin Y, et al. Analytical and Bioanalytical Chemistry, 2013, 405(29)：268.
[24] Chin Y W，Kang W K，Jang H W，et al. J. Ind. Microbiol. Biotech.，2016，43(11)：1517.