光谱学与光谱分析 |
|
|
|
|
|
Quantitative Measurement of Fe(Ⅱ) in Human Blood with Spectral Analysis Method |
XU Xiao-feng1, ZHAO Fu-li1*, WANG Shuang1,YE Bao-hui2 |
1. School of Physics Science and Engineering Technology, Sun Yat-sen University, Guangzhou 510275, China 2. School of Chemistry and Chemical Engineering, Sun Yat-sen University, Guangzhou 510275, China |
|
|
Abstract Since the absorption spectra of Fe(Ⅱ) ions solutions vary with the concentration of Fe(Ⅱ) irons, the authors tried to establish a direct measurement of active haemachrome in human blood through spectral analysis, and successfully made an experiment on it. The authors prepared Fe(Ⅱ) ions solutions with various concentrations chemically, using 1,10-phenanthroline (phen) as chromogenic agent. After the absorption spectra of these solutions were analyzed, Gaussion fitting at absorption peaks was performed to them. The linear fitting figure showed that the areas of Gaussion fitting curves correspond to the concentrations of Fe(Ⅱ) irons. Thus, the concentration of Fe(Ⅱ) in human blood was figured out in this way, and the result fitted quite well the actual concentration. As an optimized method, this creative idea is more accurate and precise. It will probably contribute to the development of new devices for measuring Fe(Ⅱ) ions in human blood.
|
Received: 2007-08-12
Accepted: 2007-11-16
|
|
Corresponding Authors:
ZHAO Fu-li
E-mail: stszfl@mail.sysu.edu.cn
|
|
[1] KONG Xiang-rui(孔祥瑞). The Effect of Trace Element in Nutrition, Physiology and Clinical Application and Technology and Technology Press(必需微量元素的营养、生理及临床意义). Hefei: Anhui Science and Technology Press(合肥:安徽科技出版社), 1982. 194. [2] JIAO Zhi-ping(焦治平). Anhui Medical and Pharmaceutical Journal(安徽医药),2002, 6(1): 42. [3] HE Bang-ping, WU Qiu-ye, PAN Ya-ju, et al(何邦平,吴秋业,潘亚菊,等). Journal of Chinese Clinical Medicine(中华临床医药),2004, 5(7): 11. [4] Ministry of Health in People’s Republic of China(中华人民共和国卫生部医政司编). National Regulation for Clinic(Second Edition)(全国临床检验操作规程,第2版). Nanjing: Southeast University Press(南京:东南大学出版社),1997. 200. [5] CAO Jian-ming, SHEN Cai-cheng, LI Min(曹建明,沈财成,李 敏). China Public Health(中国公共卫生),2002, 18(11): 1384. [6] CAO Jian-ming, XIAO Hong-wu, JIANG Hong-ying, et al(曹建明,肖洪武,姜红鹰,等). Chinese Journal of Medical Laboratory Technology(中华检验医学杂志),2004, 27(3): 137. [7] DONG Shun-fu, ZHU Zhi-guo, LIU Wei-ming, et al(董顺福,朱志国,刘伟明,等). Spectroscopy and Spectral Analysis (光谱学与光谱分析),2001, 21(3): 391. [8] LI Ren-qiang, CHEN Yao, JIANG Feng-yi(李任强,陈 瑶,江凤仪). Spectroscopy and Spectral Analysis(光谱学与光谱分析),2004, 24(1): 95. [9] LI Jin-min(李进民). Journal of Datong Vocational College(大同职业技术学院学报),2004, 18(2): 72. [10] YAO Qun-feng, XU Shun-qing, ZHOU Yi-kai(姚群峰,徐顺清,周宜开). Chinese Journal of Health Laboratory Technology(中国卫生检验杂志),2000, 10(1): 3. [11] Michael Hollas J. High Resolution Spectroscopy. John Wiley & Sons, 1982. |
[1] |
BAI Xi-lin1, 2, PENG Yue1, 2, ZHANG Xue-dong1, 2, GE Jing1, 2*. Ultrafast Dynamics of CdSe/ZnS Quantum Dots and Quantum
Dot-Acceptor Molecular Complexes[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(01): 56-61. |
[2] |
ZHENG Pei-chao, YIN Yi-tong, WANG Jin-mei*, ZHOU Chun-yan, ZHANG Li, ZENG Jin-rui, LÜ Qiang. Study on the Method of Detecting Phosphate Ions in Water Based on
Ultraviolet Absorption Spectrum Combined With SPA-ELM Algorithm[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(01): 82-87. |
[3] |
LIU Jia, ZHENG Ya-long, WANG Cheng-bo, YIN Zuo-wei*, PAN Shao-kui. Spectra Characterization of Diaspore-Sapphire From Hotan, Xinjiang[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(01): 176-180. |
[4] |
GU Yi-lu1, 2,PEI Jing-cheng1, 2*,ZHANG Yu-hui1, 2,YIN Xi-yan1, 2,YU Min-da1, 2, LAI Xiao-jing1, 2. Gemological and Spectral Characterization of Yellowish Green Apatite From Mexico[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(01): 181-187. |
[5] |
ZHENG Ni-na1, 2*, XIE Pin-hua1, QIN Min1, DUAN Jun1. Research on the Influence of Lamp Structure of the Combined LED Broadband Light Source on Differential Optical Absorption Spectrum
Retrieval and Its Removing Method[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(11): 3339-3346. |
[6] |
DUAN Ming-xuan1, LI Shi-chun1, 2*, LIU Jia-hui1, WANG Yi1, XIN Wen-hui1, 2, HUA Deng-xin1, 2*, GAO Fei1, 2. Detection of Benzene Concentration by Mid-Infrared Differential
Absorption Lidar[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(11): 3351-3359. |
[7] |
FANG Zheng, WANG Han-bo. Measurement of Plastic Film Thickness Based on X-Ray Absorption
Spectrometry[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(11): 3461-3468. |
[8] |
HUANG Li, MA Rui-jun*, CHEN Yu*, CAI Xiang, YAN Zhen-feng, TANG Hao, LI Yan-fen. Experimental Study on Rapid Detection of Various Organophosphorus Pesticides in Water by UV-Vis Spectroscopy and Parallel Factor Analysis[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(11): 3452-3460. |
[9] |
WANG Peng1, GAO Yong-bao1*, KOU Shao-lei1, MEN Qian-ni1, ZHANG Min1, HE Tao1, YAO Wei2, GAO Rui1, GUO Wen-di1, LIU Chang-rui1. Multi-Objective Optimization of AAS Conditions for Determination of Gold Element Based on Gray Correlation Degree-RSM Model[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(10): 3117-3124. |
[10] |
JIA Yu-ge1, YANG Ming-xing1, 2*, YOU Bo-ya1, YU Ke-ye1. Gemological and Spectroscopic Identification Characteristics of Frozen Jelly-Filled Turquoise and Its Raw Material[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(09): 2974-2982. |
[11] |
YANG Xin1, 2, XIA Min1, 2, YE Yin1, 2*, WANG Jing1, 2. Spatiotemporal Distribution Characteristics of Dissolved Organic Matter Spectrum in the Agricultural Watershed of Dianbu River[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(09): 2983-2988. |
[12] |
CHANG Zhen1, ZHONG Ming-yu2*, SU Jing-ming1, 2, SI Fu-qi1, WANG Yu3, ZHOU Hai-jin1, DOU Ke1, ZHANG Quan1. Study on the Reconstructing the NO2 Gas Distribution in a Vertical Plane Using MAX-DOAS[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(08): 2413-2418. |
[13] |
LIU Xian-yu1, YANG Jiu-chang1, 2, TU Cai1, XU Ya-fen1, XU Chang3, CHEN Quan-li2*. Study on Spectral Characteristics of Scheelite From Xuebaoding, Pingwu County, Sichuan Province, China[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(08): 2550-2556. |
[14] |
TIAN Si-di1, WANG Zhen1, DU Yan-jun2, DING Yan-jun1, PENG Zhi-min1*. High Precision Measurement of Spectroscopic Parameters of CO at 2.3 μm Based on Wavelength Modulation-Direct Absorption Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(07): 2246-2251. |
[15] |
LIANG Wen-ke, WEI Guang-fen, WANG Ming-hao. Research on Methane Detection Error Caused by Lorentzian Profile Approximation[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(06): 1683-1689. |
|
|
|
|