|
|
|
|
|
|
| Articular Cartilage Optical Clearing Research by Raman Spectroscopy |
| GAO Hao, ZHAI Ming-yang, SHANG Lin-wei, ZHAO Yuan, XU Hao, WANG Xiao, YIN Jian-hua* |
| Department of Biomedical Engineering, Nanjing University of Aeronautics and Astronautics, Nanjing 210016, China |
|
|
|
|
Abstract To investigate deeper microscopic information of articular cartilage and subchondral bone components in situ, Raman spectroscopy combined with tissue optical clearing technique was used to study the clearing effect of articular cartilage. Raman spectra of the cartilage adhered on femur ends in canine knees were collected after they were impregnated at different time and different concentrations of clearing agents, glycerol and iohexol. The integrated Raman intensity ratio of phosphate group (920~960 cm-1) to the amide Ⅰ band (1 595~1 700 cm-1) was obtained to seek the rule of tissue optical clearing at different impregnating time (10～60 min) and different concentrations of clearing agents (40%, 60%, 80% and 100% for glycerol, 50, 150, 250, 350 mg·mL-1 for iohexol), which is to get the optimum concentration and time. It was found that the both agents could enhance the Raman signal compared to the case of no clearing agent. With equal time for impregnating, glycerol and iohexol were able to obtain good results at 60% and 150 mg·mL-1, respectively. Under different concentrations, the clearing effect of glycerol was the strongest in 20 min, while that of iohexol was enhanced after 50-min impregnation in general. In conclusion, the Raman scattering information of subchondral bone can be directly detected when the clearing agents penetrating through cartilage, which will provide a novel way and a potential solution into study on osteoarthritis etiology in depth.
|
|
Received: 2017-08-28
Accepted: 2018-01-14
|
|
|
|
Corresponding Authors:
YIN Jian-hua
E-mail: yin@nuaa.edu.cn
|
|
[1] Cook J L, Kuroki K, Visco D, et al. Osteoarthritis and Cartilage, 2010, 18: S66.
[2] Fields M. Characterisation of Articular Cartilage by Micro-Beam Raman Spectroscopy. UCL (University College London), 2016.
[3] Kumar R, Gr?nhaug K M, Afseth N K, et al. Analytical and Bioanalytical Chemistry, 2015, 407(26): 8067.
[4] Yin J, Xia Y. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 2014, 133: 825.
[5] Olumegbon I A, Oloyede A, Afara I O. Applied Spectroscopy Reviews, 2017, 52(6): 541.
[6] WANG Xiao, WU Yue-chao, HE Jun-hao, et al(王 潇, 吴曰超, 何俊豪, 等). Chinese Journal of Light Scattering(光散射学报), 2016, 28(2): 185.
[7] Takahashi Y, Sugano N, Takao M, et al. Journal of the Mechanical Behavior of Biomedical Materials, 2014, 31: 77.
[8] Bonifacio A, Beleites C, Vittur F, et al. Analyst, 2010, 135(12): 3193.
[9] Day J S, Ding M, Linden J C V D, et al. Journal of Orthopaedic Research, 2001, 19(5): 914.
[10] Zhu D, Larin K V, Luo Q, et al. Laser & Photonics Reviews, 2013, 7(5): 732.
[11] Soham Ghosh, Dominik R Haudenschild, Sarah Calve, et al. ORS 2017 Annual Meeting，2017.
[12] Bykov A, Hautala T, Kinnunen M, et al. Journal of Biophotonics, 2016, 9(3): 270.
[13] Schulmerich M V, Cole J H, Dooley K A, et al. Journal of Biomedical Optics, 2008, 13(2): 021108.
[14] GAO Hao, WANG Xiao, SHANG Lin-wei, et al(高 浩，王 潇，尚林伟，等). Spectroscopy and Spectral Analysis(光谱学与光谱分析)，2018，38(6)：1933.
[15] Zhao J, Lui H, McLean D I, et al. Applied Spectroscopy, 2007, 61(11): 1225.
[16] Buchwald T, Niciejewski K, Kozielski M, et al. Journal of Biomedical Optics, 2012, 17(1): 017007.
[17] Tromberg B J, Shah N, Lanning R, et al. Neoplasia, 2000, 2(1): 26
[18] Genina E A, Bashkatov A N, Sinichkin Yu P, et al. J. of Biomedical Photonics & Eng., 2015, 1(1): 22.
[19] Bykova A, Hautala T, Kinnunen M, et al. European Conferences on Biomedical Optics. International Society for Optics and Photonics, 2015.
[20] Esmonde-White K A, Esmonde-White F W, Morris M D, et al. Analyst, 2011, 136(8): 1675.
[21] Mohidin B, Gikas P D, Kerns J G, et al. Bone Joint J, 2013, 95(Sup. 13): 44.
[22] Vardaki M Z, Papachristou D J, Megas P, et al. Bone, 2012, 50: S190.
[23] Hirshburg J M, Ravikumar K M, Hwang W, et al. Journal of Biomedical Optics, 2015, 15(5): 055002.
[24] Sdobnov A, Darvin M E, Lademann J, et al. Journal of Biophotonics, 2017，10(9)：1115. |
| [1] |
FU Yun-peng, QI Ying, HU Xiao-peng, TONG Rui, FANG Guo-zhen*, WANG Shuo. Study on the Determination of Basic Orange Ⅱ and Acid Orange Ⅱ in Food by TLC-SERS[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(08): 2419-2424. |
| [2] |
LIN Xue-liang1, LIN Duo1, 2, QIU Su-fang3, GE Xiao-song1, PAN Jian-ji3, WU Qiong1, LIN Hui-jing1, HUANG Hao2*. Detection of Nasopharyngeal Carcinoma Based on Human Saliva Surface-Enhanced Raman Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(08): 2430-2434. |
| [3] |
WANG Ning1, 2, WANG Chi1, BIAN Hai-yi2, WANG Jun3, WANG Peng2, BAI Peng-li3, YIN Huan-cai3, TIAN Yu-bing2, GAO Jing2*. The Identification Method of Blood by Applying Hilbert Transform to Extract Phase Information of Raman Spectra[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(08): 2412-2418. |
| [4] |
ZHONG Hang, XU Jin-song, TAO Ran, DU Xiao-qing, CHEN Jun*, LIAO Jun-sheng*. Corrosion Resistance of Gold Surface Investigated by Surface Enhanced Raman Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(08): 2435-2440. |
| [5] |
GONG Nan1, CAO Xian-wen1, SUN Cheng-lin1, FANG Wen-hui3, YUAN Ju-hui2, GAO Shu-qin1, LI Zuo-wei1, CHEN Wei2*, FU Hao-yang1*. Effects of External Fields on CC Atomic Vibrations Modulated by Electron Band Gap[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(08): 2462-2467. |
| [6] |
LIU Lu-yao1, ZHANG Bing-jian1,2*, YANG Hong3, ZHANG Qiong3. The Analysis of the Colored Paintings from the Yanxi Hall in the Forbidden City[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(07): 2054-2063. |
| [7] |
CHEN Sheng, ZHANG Xun, XU Feng*. Study on Cell Wall Deconstruction of Pinus Massoniana during Dilute Acid Pretreatment with Confocal Raman Microscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(07): 2136-2142. |
| [8] |
HE Qing1, JIANG Qin1, XING Li-da2, 3, AN Yan-fei1, HOU Jie4, HU Yi5. Microstructure and Raman Spectra Characteristics of Dinosaur Eggs from Qiyunshan, Anhui Province[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(07): 2143-2148. |
| [9] |
CAI Zong-qi1, FENG Wei-wei1, 2*, WANG Chuan-yuan1. The Study of Oil Film Thickness Measurement on Water Surface Based on Laser Raman Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(06): 1661-1664. |
| [10] |
WU Jun, YOU Jing-lin*, WANG Yuan-yuan, WANG Jian, WANG Min, Lü Xiu-mei. Raman Spectroscopic Study of Li2B4O7 Crystal and Melt Structure[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(06): 1736-1740. |
| [11] |
ZHANG Lu-tao, ZHOU Guang-ming*, ZHANG Cai-hong, LUO Dan. The Preparation of the New Membrane-Like Gold Nanoparticles Substrate and the Study of Its Raman Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(06): 1741-1746. |
| [12] |
CHEN Si-yuan1, YANG Miao1, LIU Xiao-yun2*, ZHA Liu-sheng1*. Study on Au@Ag Core-Shell Composite Bimetallic Nanorods Laoding Filter Paper as SERS Substrate[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(06): 1747-1752. |
| [13] |
MA Ying1, WANG Qi2, QIU Zhi-li1*, LU Tai-jin3, LI Liu-fen1, CHEN Hua3, DENG Xiao-qin1, BO Hao-nan1. In-Situ Raman Spectroscopy Testing and Genesis of Graphite Inclusions in Alluvial Diamonds from Hunan[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(06): 1753-1757. |
| [14] |
LIU Jia1, YANG Ming-xing1, 2*, DI Jing-ru1, 2, HE Chong2. Spectra Characterization of the Uvarovite in Anorthitic Jade[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(06): 1758-1762. |
| [15] |
OUYANG Ai-guo, ZHANG Yu, TANG Tian-yi, LIU Yan-de. Study on Density, Viscosity and Ethanol Content of Ethanol Diesel Based on Raman Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(06): 1772-1778. |
|
|
|
|
