| 光谱学与光谱分析 |
|
|
|
|
|
| Qualitative and Quantitative Studies on Artemisinin with Raman Spectroscopy |
| KONG Meng-hong, WU Du-xuan, CHEN Xiang-bai* |
| School of Science and Laboratory of Optical Information Technology, Wuhan Institute of Technology, Wuhan 430205, China |
|
|
|
|
Abstract Artemisinin, one of the most powerful new generation antimalarial drugs, is an unique sesquiterpene lactone compound extracted from traditional Chinese drug Artemisa annua L, which contains specific endoperoxide bridge. In this study, the Raman scattering of artemisinin in the spectral range of 100～3 500 cm-1 has been investigated. The analysis suggests that the phonon mode at 724 cm-1 would be directly correlated with a representative vibrational mode of the ring containing endoperoxide bridge, thus it can be applied for Raman detection of endoperoxide bridge in artemisinin. The phonon mode at 1 734 cm-1 would be directly correlated with a representative vibrational mode of the lactone ring, thus can be applied for further identification of artemisinin with Raman spectroscopy. Also both of these two phonon modes can be easily observed by Raman experiment; therefore they are good representative phonon modes for quick qualitative analysis of artemisinin by Raman spectroscopy. In addition, by investigating the relative intensity ratio of the two representative phonon modes at 724 and 1 734 cm-1, the Raman method can be applied for quantitative analysis of artemisinin purity. Compared with the commonly used high performance liquid chromatography method, the Raman method is much more powerful: it is faster, more convenient, more accurate, and can be applied for the analysis of homogeneity of purity for artemisinin samples. Furthermore, the qualitative and quantitative analysis of artemisinin purity would be very helpful for quantitative analysis of the quality of Chinese drug Artemisa annua L with Raman spectroscopy.
|
|
Received: 2016-03-07
Accepted: 2016-08-05
|
|
|
|
Corresponding Authors:
CHEN Xiang-bai
E-mail: xchen@wit.edu.cn
|
|
[1] Klayman D L. Science, 1985, 228: 1049.
[2] Research Group on the Structure of Artemisinin(青蒿素结构研究协作组). Chinese Science Bulletin(科学通报), 1977, 142: 22.
[3] Pandey N, Pandey-Rai S. Protoplasma, 2016, 253(1): 15.
[4] LIU Chun-zhao, WANG Yu-chun, OUYANG Fan, et al(刘春朝，王玉春，欧阳藩, 等). Progress in Chemistry(化学进展), 1999, 1: 41.
[5] Stckel S, Kirchhoff J, Neugebauer U, et al. Journal of Raman Spectroscopy, 2016, 47: 89.
[6] Moroni L, Gellini C, Miranda M M, et al. Journal of Raman Spectroscopy, 2008, 39: 276.
[7] Vo-Dinh T, Yan F, Wabuyele M B. Journal of Raman Spectroscopy, 2005, 36: 640.
[8] FANG Yi-xing, ZHU Zi-ying, HE Da-jun(方一行，朱自莹，何大钧). Acta Chimica Sinica(化学学报), 1984, 42(12): 1312.
[9] LIU Jing-ming, NI Mu-yun, FAN Ju-fen, et al(刘静明，倪慕云，樊菊芬，等). Acta Chimica Sinica(化学学报), 1979, 37(2): 129.
[10] Gu Renao, Hu Xiao. Acta Chimica Sinica, 1993, 51(5): 481.
[11] LI Chao, WANG Rui-li, ZHANG Li-feng, et al(李 超，王锐利，张丽锋，等). Chinese Remedies & Clinical(中国药物与临床), 2011, 7: 748.
[12] LIU Xiao-wei, WU Wei, ZHU Dong-yin, et al(刘晓伟，吴 维，朱冬寅，等). China Food Safety Magazine(食品安全导刊), 2010，(Z1): 38.
[13] Esfandiary R, Middaugh C R. Methods in Molecular Biology, 1995, 40: 91.
[14] HU Yong-chuan, TIAN Xiao-xin, LIU Lei, et al(胡咏川，田晓鑫，刘 蕾，等). China Journal of Chinese Materia Medica(中国中药杂志), 2012, 8: 1066.
[15] XU Yi, FAN Qi, SHENG Jing, et al(徐 溢，范 琪，盛 静，等). Chinese Journal of Pharmaceutical Analysis(药物分析杂志), 2013, 9: 1465. |
| [1] |
LIU Lu-yao1, ZHANG Bing-jian1,2*, YANG Hong3, ZHANG Qiong3. The Analysis of the Colored Paintings from the Yanxi Hall in the Forbidden City[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(07): 2054-2063. |
| [2] |
CHEN Sheng, ZHANG Xun, XU Feng*. Study on Cell Wall Deconstruction of Pinus Massoniana during Dilute Acid Pretreatment with Confocal Raman Microscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(07): 2136-2142. |
| [3] |
HE Qing1, JIANG Qin1, XING Li-da2, 3, AN Yan-fei1, HOU Jie4, HU Yi5. Microstructure and Raman Spectra Characteristics of Dinosaur Eggs from Qiyunshan, Anhui Province[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(07): 2143-2148. |
| [4] |
CAI Zong-qi1, FENG Wei-wei1, 2*, WANG Chuan-yuan1. The Study of Oil Film Thickness Measurement on Water Surface Based on Laser Raman Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(06): 1661-1664. |
| [5] |
WU Jun, YOU Jing-lin*, WANG Yuan-yuan, WANG Jian, WANG Min, Lü Xiu-mei. Raman Spectroscopic Study of Li2B4O7 Crystal and Melt Structure[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(06): 1736-1740. |
| [6] |
ZHANG Lu-tao, ZHOU Guang-ming*, ZHANG Cai-hong, LUO Dan. The Preparation of the New Membrane-Like Gold Nanoparticles Substrate and the Study of Its Raman Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(06): 1741-1746. |
| [7] |
CHEN Si-yuan1, YANG Miao1, LIU Xiao-yun2*, ZHA Liu-sheng1*. Study on Au@Ag Core-Shell Composite Bimetallic Nanorods Laoding Filter Paper as SERS Substrate[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(06): 1747-1752. |
| [8] |
MA Ying1, WANG Qi2, QIU Zhi-li1*, LU Tai-jin3, LI Liu-fen1, CHEN Hua3, DENG Xiao-qin1, BO Hao-nan1. In-Situ Raman Spectroscopy Testing and Genesis of Graphite Inclusions in Alluvial Diamonds from Hunan[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(06): 1753-1757. |
| [9] |
LIU Jia1, YANG Ming-xing1, 2*, DI Jing-ru1, 2, HE Chong2. Spectra Characterization of the Uvarovite in Anorthitic Jade[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(06): 1758-1762. |
| [10] |
OUYANG Ai-guo, ZHANG Yu, TANG Tian-yi, LIU Yan-de. Study on Density, Viscosity and Ethanol Content of Ethanol Diesel Based on Raman Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(06): 1772-1778. |
| [11] |
PENG Heng, LIU Shuai, CHEN Xiang-bai*. Raman Study of Perovskite (C6H5CH2NH3)2PbBr4[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(06): 1763-1765. |
| [12] |
ZHONG Qian1, 2, 3, WU Qiong2, 3, LIAO Zong-ting1, 2, 3*, ZHOU Zheng-yu1, 2, 3. Vibrational Spectral Characteristics of Ensignia Actinolite Jade from Guangxi, China[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(06): 1786-1792. |
| [13] |
XU Wei-jie1, WU Zhong-chen1, 2*, ZHU Xiang-ping2, ZHANG Jiang1, LING Zong-cheng1, NI Yu-heng1, GUO Kai-chen1. Classification and Discrimination of Martian-Related Minerals Using Spectral Fusion Methods[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(06): 1926-1932. |
| [14] |
GAO Hao1, WANG Xiao1, SHANG Lin-wei1, ZHAO Yuan1, YIN Jian-hua1*, HUANG Bao-kun2*. Design and Application of Small NIR-Raman Spectrometer Based on Dichroic and Transmission Collimating[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(06): 1933-1937. |
| [15] |
LU Shu-hua1, 2*, WANG Yin-shu3. Developments in Detection of Explosives Based on Surface Enhanced Raman Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(05): 1412-1419. |
|
|
|
|
