光谱学与光谱分析 |
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Raman Spectroscopic Investigation on the Interactions between Liver Cancer Cells (SMMC-7721) and Fufang Luxiancao Particles |
ZHANG Jin-yan, GUO Jian-yu, CAI Wei-ying, SUN Zhen-rong* |
Sate Key Laboratory of Precision Spectroscopy and Depertment of Physics, East China Normal University, Shanghai 200062, China |
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Abstract Luxiancao is a new medicine for liver cancer, and is purely natural botanical. It has good curative effects and few side effects. The curative mechanism of Luxiancao is unknown. In the present paper, the authors used a 514.5 nm laser to measure the changes in the Raman spectrum of liver cancer cells (SMMC-7721) treated by Luxiancao at different concentrations. The study can help us know more about the mechanism, efficiency and side effects of Luxiancao. The results show that significant changes were observed in the cells’ Raman spectra after reacting with Luxiancao. The intensities at 785 and 1 092 cm-1,corresponding to DNA phosphate backbone vibration, were reduced; and the Raman bands for the bases A and G at 1 312 and 1 585 cm-1 also decreased, indicating that Luxiancao may be inserted in DNA bases and influence the DNA replications, resulting in the reduction in DNA content and breaking of the DNA strands. Besides, the intensity of 1 360 cm-1,belonging to Trp, decreased gradually and disappeared in the end, indicating the Trp of cancer cells began to be exposed when adding in Luxiancao. The bands at 1 004 cm-1 for Phe and 1 656 cm-1 for proteins α-helix also decreased, suggesting that there were changes in the structure of protein and circumstance of amino acid. Moreover, the effects on cancer cells were enhanced gradually with the HCPT concentration increasing. Since a Raman spectrum is a chemical fingerprint of a sample, the different concentration dependent changes in the Raman spectra of individual cells due to reacting with Luxiancao can overcome the limitations of other detection systems used for quantitative and qualitative analysis of the drug.
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Received: 2007-09-22
Accepted: 2007-12-28
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Corresponding Authors:
SUN Zhen-rong
E-mail: zrsun@phy.ecnu.edu.cn
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[1] WANG Shi-hong, WEI Wei, XU Du-juan, et al(王世宏,魏 伟,许杜娟,等). Anhui Medical and Pharmaceutical Journal(安徽医药), 2006, 10(1): 8. [2] SONG Wen-guang, WANG Yi-feng, ZHANG Zhi, et al(宋文广,王毅峰,张 志, 等). Shaanxi Journal of Traditional Chinese Medicine(陕西中医), 2006, 27(5): 629. [3] CHEN Long-fei, CHEN Tao(陈龙飞,陈 涛). Chinese Journal of the Practical Chinese and Modern Medicine(中华实用中西医杂志), 2003, 6(14): 2123. [4] GUO Jian-yu, SUN Zhen-rong, WU Liang-ping, et al(郭建宇,孙真荣,吴良平,等). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2006, 26(3): 460. [5] TANG Yu-long, GUO Zhou-yi(唐玉龙,郭周义). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2006, 26(4): 653. [6] YU Ge, ZHANG Pan, TAN En-zhong, et al(于 舸,张 攀,谭恩忠,等). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2007, 27(2): 295. [7] Hanlon E B, Manoharan R, Koo T W, et al. Phys. Med. Biol., 2000, 45: R1. [8] Ramasamy M, Yang W, Feld M S. Spectrochimica Acta Part A, 1996, 52: 215. [9] Sharon R H, William B C, Andre K B, et al. Cancer Lett., 1996, 110: 35. [10] Notingher I, Verrier S, Romanskab H, et al. Spectroscopy-An Interational Journal, 2002, 16(2): 43. [11] Verrier S, Notingher I, Polak J M, et al. Biopolymers, 2004, 74(1-2): 157. [12] Notingher I, Verrier S, Haque S, et al. Biopolymers, 2003, 72(4): 230. [13] XU Yi-ming(许以明). Raman Spectroscopy in Application of Structure Biology(拉曼光谱及其在结构生物学中的应用). Beijing: Chemical Industry Press(北京: 化学工业出版社), 2005. [14] KE Wei-zhong, ZHOU Dian-feng(柯惟中,周殿凤). Chinese Journal of Spectroscopy Laboratory(光谱实验室), 2004, 21(3): 432. [15] LIU Song-hao, MENG Yao-yong(刘颂豪,孟耀勇). Acta Optica Sinica(光学学报), 2000, 20(4): 529. [16] XU Yi-ming, ZHANG Zhi-yi, XU Guo-rui(许以明,张志义,徐国瑞). Chinese Science Bulletin(科学通报), 1989, 34(19): 1500. [17] ZHAO Hong-xia, XU Yi-ming, ZHANG Zhi-yi(赵红霞,许以明,张志义). Chinese Science Bulletin(科学通报), 1998, 43: 957. [18] LI Wei, CHEN Wu-gao, LIANG Yong-mao(李 蔚,陈五高,梁永茂). Chinese Journal of Laser Medicine & Surgery(中国激光医学杂志), 1998, 7(2): 102. |
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