| 光谱学与光谱分析 |
|
|
|
|
|
| Study on the Interaction of Cordycepin and DNA |
| PENG Jun-feng，LING Jian-ya，ZHANG Han-xing，ZHANG Chang-kai* |
| The State Key Lab of Microbial Technology，Shandonｇ Universitｙ，Ji'nan 250100，China |
|
|
|
|
Abstract The absorbance, fluorescence and Scatchard plots methods as well as the effect of phosphate group on the fluorescence intensity of the cordycepin-DNA-EB system were used to study the interaction of the antitumor compound cordycepin and DNA. It is obvious tiat there is hyperchromic effect and hypochromic effect with slight red shift on the subtracted UV spectrum. It proves that the adenine base of cordycepin can be inserted into the double-helix of DNA. The results of the fluorescence intensity that decreases after increases with a little blue shift on the fluorescence spectrum also proved this. At the same time the phosphate can affect the cordycepin-DNA-EB system. Finally that the result depended on the Scatchard equation indicates that cordycepin reacts electrostatically on phosphate backbone of DNA. There also exists an intercalation into the double-helix of DNA.
|
|
Received: 2003-05-16
Accepted: 2003-10-26
|
|
|
|
Corresponding Authors:
ZHANG Chang-kai
|
|
| Cite this article: |
|
PENG Jun-feng,LING Jian-ya,ZHANG Han-xing, et al. Study on the Interaction of Cordycepin and DNA [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2004, 24(07): 858-861.
|
|
|
|
| URL: |
|
http://www.gpxygpfx.com/EN/Y2004/V24/I07/858 |
[1] Ling Jian-ya, Sun Ying-jie, Zhang Hua et al. J. Bioscience Bioengineering，2002, 94(4): 371.
[2] Lee M, Ｋrowick Ｋ et al. J. Am. Chem. Soc.，1988, 110: 3641.
[3] SHEN Tonｇ(沈 同编著). Biochemistrｙ(生物化学(上册)). Beijinｇ：People′s Edｕcation Press(北京：人民教育出版社)，1989.
[4] Wu K C, Lippard S J. Biochemistry, 1976, 15(19): 4339.
[5] Lepecq J B, Paoletti C. J. Mol. Biol., 1967, 27: 87.
[6] GUO Mao-lin，YANG Pin，YANG Bin-shenｇ(郭茂林，杨 频，杨斌盛等). Science in China B(中国科学B辑)，1995，5. 517.
|
| [1] |
MA Ping1, 2, Andy Hsitien Shen1*, ZHONG Yuan1, LUO Heng1. Study on UV-Vis Absorption Spectra of Jadeite From Different Origins[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(06): 1827-1831. |
| [2] |
WANG Xue-pei1, 2, ZHANG Lu-wei1, 2, BAI Xue-bing3, MO Xian-bin1, ZHANG Xiao-shuan1, 2*. Infrared Spectral Characterization of Ultraviolet Ozone Treatment on Substrate Surface for Flexible Electronics[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(06): 1867-1873. |
| [3] |
LUO Heng, Andy Hsitien Shen*. Based on Color Calculation and In-Situ Element Analyze to Study the Color Origin of Purple Chalcedony[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(06): 1891-1898. |
| [4] |
LÜ Yang, PEI Jing-cheng*, GAO Ya-ting, CHEN Bo-yu. Chemical Constituents and Spectra Characterization of Gem-Grade
Triplite[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(04): 1204-1208. |
| [5] |
WU Shuang-cheng1, 3, CHENG Pu2, LI Ting-ting2, YANG Yang2, TIE Fu-de1*, JIN Pu-jun2*. Multispectral Analysis on Lacquer Films of Boxes Excavated From Haiqu Cemetery During the Han Dynasties[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(04): 1150-1155. |
| [6] |
SONG Peng1, CAI Yuan-min1*, GENG Xiao-jun2, GUO Hua1, JI Han-wu1, ZHANG Guo-qing3. Study on Scattering Transmission Characteristics of Wireless UV Communication Based on Particle Size Distribution[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(03): 970-977. |
| [7] |
LI Qing-bo1, WEI Yuan1, CUI Hou-xin2, FENG Hao2, LANG Jia-ye2. Quantitative Analysis of TOC in Water Quality Based on UV-Vis Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(02): 376-380. |
| [8] |
CHEN Feng-xia1, YANG Tian-wei2, LI Jie-qing1, LIU Hong-gao3, FAN Mao-pan1*, WANG Yuan-zhong4*. Identification of Boletus Species Based on Discriminant Analysis of Partial Least Squares and Random Forest Algorithm[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(02): 549-554. |
| [9] |
WAN Xiao-ming1, 2, ZENG Wei-bin1, 2, LEI Mei1, 2, CHEN Tong-bin1, 2. Micro-Distribution of Elements and Speciation of Arsenic in the Sporangium of Pteris Vittata[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(02): 478-482. |
| [10] |
FU Rui-yun1, FU Xiao-hui1, ZHANG Wen-bo1,4*, LI Dong-qing2, GUAN Cheng3,4, ZHANG Hou-jiang3,4. A Qualitative and Quantitative NIRs Study on Larch Wood Surface Color Change by UV Light Irradiation[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(01): 56-61. |
| [11] |
LU Li-min, SHI Bin, TANG Tian-yu, ZHAO Xian-hao, WEI Xiao-nan, TANG Yan-lin*. Spectral Analysis of Epinephrine Molecule Based on Density Functional Theory[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(01): 248-252. |
| [12] |
ZHONG Yuan, QU Meng-wen, Andy Hsitien Shen*. Comparison of Chemical Composition and Spectroscopy of Purple- Brownish Red Garnet From Zambia, Tanzania and Australia[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(01): 184-190. |
| [13] |
LIN Yan1, SU Jun-hong1*, TANG Yan-lin2, YANG Dan3. Ultraviolet Spectrum and Excitation Properties Calculations of Vitamin C Based on Density Functional Theory[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(01): 304-309. |
| [14] |
WANG Qian, Norsang Gelsor*, Tsoja Wangmu, Lagba Tunzhup, Pu Dopwang, LIU Juan, ZHOU Yi, ZE Xi. Solar Radiation Observation During a Solar Eclipse in Tibet[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2021, 41(12): 3892-3900. |
| [15] |
LIU Jia-cheng1, 2, HU Bing-liang1, YU Tao1*, WANG Xue-ji1, DU Jian1, LIU Hong1, LIU Xiao1, HUANG Qi-xing3. Nonlinear Full-Spectrum Quantitative Analysis Algorithm of Complex Water Based on IERT[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2021, 41(12): 3922-3930. |
|
|
|
|
