光谱学与光谱分析 |
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Raman Spectral Study of Nitrosyhemoglobin and Several Other Hemoglobins |
CEN Yan1,2, ZHANG Ren3, YAO Wen-hua3, MA Jiong1,2, CHEN Ji-yao1, 4* |
1. Department of Physics, Fudan University, Shanghai 200433, China2. State Key Laboratory of Applied Surface Physics, Fudan University, Shanghai 200433, China3. Analysis and Measurement Center, Fudan University, Shanghai 200433, China4. State Key Laboratory for Advanced Photonic Materials and Devices, Fudan University, Shanghai 200433, China |
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Abstract The Raman spectra of nitrosyhemoglobin(NOHb), methemoglobin(MetHb), oxygenated hemoglobin(oxyHb),and deoxygenated hemoglobin(deoxyHb) were comparatively studied. The results show that the Raman spectra with 632.8 nm excitation can be used to detect the R state (oxyHb) to T state (deoxyHb) transition. The difference of NOHb and MetHb in their electron spin state was clearly demonstrated in their Raman spectra with 632.8 nm excitation, suggesting that Raman spectra provide a new simple way to measure and distinguish NOHb and MetHb.
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Received: 2003-09-26
Accepted: 2004-01-16
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Corresponding Authors:
CHEN Ji-yao
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Cite this article: |
CEN Yan,ZHANG Ren,YAO Wen-hua, et al. Raman Spectral Study of Nitrosyhemoglobin and Several Other Hemoglobins[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2005, 25(03): 405-408.
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URL: |
http://www.gpxygpfx.com/EN/Y2005/V25/I03/405 |
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