光谱学与光谱分析 |
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Effects of Different Levels of Vitamin C on UV Radiation-Induced DNA Damage |
ZHOU Dian-feng, KE Wei-zhong*, CHEN Nian-feng, JI Kang |
Jiangsu Provincial Key Lab for Photoelectric Technology, Analysis and Test Center, Nanjing Normal University, Nanjing 210097, China |
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Abstract The Raman spectra of DNA in different levels of vitamin C with 10 min ultraviolet radiation and 30 min ultraviolet radiation were reported. The intensity of ultraviolet radiation was 18.68 W·m-2. The experimental results proved that vitamin C can reduce DNA damage solely, but the effects are related to the concentration of vitamin C. When the concentration of vitamin C was lower than 0.35 mmol·L-1, DNA was protected better with the rising of vitamin C concentration during ultraviolet radiation. DNA was protected best when vitamin C was 0.35 mmol·L-1. When vitamin C concentration was higher than 0.35 mmol·L-1, vitamin C accelerated DNA damage with 10 min ultraviolet radiation. But DNA was also protected with 30 min ultraviolet radiation as vitamin C was 0.35-0.65 mmol·L-1. However, DNA was only protected slightly at high concentration of vitamin C.
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Received: 2004-09-16
Accepted: 2004-12-18
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Corresponding Authors:
KE Wei-zhong
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Cite this article: |
ZHOU Dian-feng,KE Wei-zhong,CHEN Nian-feng, et al. Effects of Different Levels of Vitamin C on UV Radiation-Induced DNA Damage [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2005, 25(12): 2012-2015.
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URL: |
https://www.gpxygpfx.com/EN/Y2005/V25/I12/2012 |
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