1. College of Science, China Agricultural University, Beijing 100094, China 2. College of Fundamental Science, North China University of Technology, Beijing 100041, China
Abstract：The Resonance Light Scattering (RLS) spectra of atrazine with yeast ribonucleic acid (yRNA) had been studied. At pH 1.50, the enhanced RLS of atrazine-yRNA system is characterized by the maximum scattering peaks at 320 nm and the RLS intensity is proportional to the concentration of yRNA. Under optima conditions, the linear ranges of the calibration were 0.6-5.0 μg·mL-1, the linear regression equation was I=25.88+140.0 c(yRNA，μg·mL-1 ), r=0.997 5, with the limit of detection 20.7 ng·mL-1(3δ). The method was applied to the determination of yRNA in synthetic samples and RNA in salt cress (Thellungiella Halophila) sample satisfactorily. Mechanism studies show that there are two interaction modes between atrazine and RNA: electrostatic mode and intercalative mode.
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