Abstract：The characteristics of resonance light scattering and absorption spectra of doxepin hydrochloride with fast green were investigated, and a new analytical method for doxepin hydrochloride was described. In the buffer solution of pH 4.0, doxepin hydrochloride can strengthen the signal of resonance light scattering of fast green. The effective factors, including the order of addition of the reagents, acidity and kinds of the buffers, and concentration of fast green were investigated. Under the optimum conditions, the scattering peaks of the system are at 344 and 486 nm，while the maximum is at 344 nm. The enhanced intensity of RLS is proportional to the concentration of doxepin hydrochloried in the range of 8.75×10-3-4.88×10-2 mg·mL-1. The detection limit for doxepin hydrochloried is 1.72×10-5 mg·mL-1. The relative standard deviation obtained from eleven determinations for a 0.025 mg·mL-1 standard solution of doxepin hydrochloried is 1.4%. The method was applied to the determination of doxepin hydrochloric in pharmaceutical preparations. The results were compared with those obtained by the reference method, and t-test showed no significant difference between the two methods.
Key words：Resonance light scattering;Doxepin hydrochloried;Fast green
吴呈珂,冯素玲*,樊静. 光谱法研究盐酸多塞平与固绿的相互作用及其分析应用[J]. 光谱学与光谱分析, 2007, 27(12): 2490-2493.
WU Cheng-ke,FENG Su-ling*,FAN Jing. Spectrometric Study on the Interaction of Doxepin Hydrochloride and Fast Green and Its Application. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2007, 27(12): 2490-2493.
[1] CHEN Xiao-xu, CHEN Guo-yu(陈晓旭，陈国羽). Journal of Chinese Modern Dermatology(中华现代皮肤科学杂志), 2005, 2(2): 134. [2] HAN Shao-wei(韩少伟). Central Plains Medical Journal(中原医刊)，2005, 32(21): 77. [3] Editorial Committee of the Pharmacopoeia of the People’s Republic of China(中华人民共和国药典委员会编). The Pharmacopoeia of the People’s Republic of China( Part Ⅱ)(中华人民共和国药典，２部). Beijing: Chemical Industry Press(北京: 化学工业出版社), 2005. 505. [4] Pasternac R F, Bustamante C, Collings P J, et al. J. Am. Chem. Soc., 1993, 115(13): 5393. [5] Huang C Z, Li K A, Tong S Y. Anal. Chem., 1996, 68(13): 2259. [6] WAN Xin-jun, LIU Jin-shui, WANG Lun(万新军, 刘金水, 王 伦). Spectroscopy and Spectral Analysis(光谱学与光谱分析)，2005, 25(5): 754. [7] RAO Zhen-hong, XIONG Yan-mei, REN Li-ping(饶震红, 熊艳梅, 任丽萍). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2004, 24(9): 1035. [8] Feng S L, Pan Z H, Fan J. Anal. Bioanal. Chem., 2005, 383(2): 255. [9] Li N B, Luo H Q, Liu S P, et al. Spectrochim. Acta Part A, 2002, 58(3): 501. [10] Long X F, Bi S P, Ni H Y, et al. Anal. Chem. Acta, 2004, 501(1): 89. [11] WANG Li-sheng, ZOU Jie-ming, JIANG Zhi-liang, et al(王力生, 邹节明, 蒋治良, 等). Spectroscopy and Spectral Analysis(光谱学与光谱分析)，2004，24(12): 1640. [12] ZOU Jie-ming, PAN Hong-cheng, JIANG Zhi-liang, et al(邹节明, 潘宏程, 蒋治良, 等). Chin. J. Anal. Chem.(分析化学)，2003，31(11): 1348.
