Reaction of Atrazine with Protein and Its Application to Detection of Protein
SHI Deng-rong1, 2, 3,ZHANG Tao1,REN Li-ping1*,YU Jin-lian3,ZHANG Chun-rong1,RAO Zhen-hong1
1. Department of Applied Chemistry, College of Science, China Agricultural University, Beijing 100094, China 2. Cold and Arid Regions Environmental and Engineering Research Institute, Chinese Academy of Sciences, Lanzhou 730000, China 3. Department of Environmental Science and Engineering, College of Tourism, Shanghai Normal University, Shanghai 200234, China
Abstract：The resonance light scattering (RLS) technique and UV-Vis absorption spectra were applied to the investigation of the interaction between atrazine and bovine serum albumin(BSA). Under acidic conditions, the formation of atrazine-BSA supermolecule by Van der Waals force and N/O—H…π hydrogen bonds leads to a red shift of absorption band and strong RLS enhancement of atrazine. The characteristics and intensity of RLS were related to the pH, the concentration of atrazine, and temperature. Under the optimum conditions, the enhanced RLS intensities are in proportion to the concentration of BSA in the range of 0.05-100 μg·mL-1. Based on the enhancement of the RLS, a simple and sensitive method for the determination of BSA was established. The detection limit (3σ) is 12 ng·mL-1. Synthesis samples were determined with satisfactory results.
石登荣1, 2, 3,张 涛1,任丽萍1*,于金莲3,张春荣1,饶震红1 . 阿特拉津与蛋白质结合反应及其在测定蛋白质中的应用[J]. 光谱学与光谱分析, 2006, 26(03): 509-512.
SHI Deng-rong1, 2, 3,ZHANG Tao1,REN Li-ping1*,YU Jin-lian3,ZHANG Chun-rong1,RAO Zhen-hong1 . Reaction of Atrazine with Protein and Its Application to Detection of Protein. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2006, 26(03): 509-512.
[1] Huang C Z, Li Y F. Analytica Chimica Acta, 2003, 500(1-2): 105. [2] Sun C G, Yang G H, Li L, et al. Journal of Chromatography B, 2004, 803(2): 173. [3] Filipov N M, Pinchuk L M, Boyd B L, et al. Toxicological Sciences: An Official Journal of the Society of Toxicology, 2005, 86(2): 324. [4] Hecker M, Park J W, Murphy M B, et al. Toxicological Sciences: An Official Journal of the Society of Toxicology, 2005, 86(2): 273. [5] Brooks D R, Clark S J, Perry J N, et al. Proc. Biol. Sci., 2005, 272(1571): 1497. [6] REN Li-ping, JIANG Shu-ren, RAO Zhen-hong, XIONG Yan-mei, ZHANG Chun-rong, WANG Dong-dong(任丽萍, 江树人, 饶震红, 熊艳梅, 张春荣, 王东冬). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2004, 24(10): 1213. [7] REN Li-ping, JIANG Shu-ren, RAO Zhen-hong, et al(任丽萍, 江树人, 饶震红, 等). Journal of Instrumental Analysis(分析测试学报), 2004, 23(6): 57. [8] ZENG Jing-ze(曾经泽). Biopharmaceutical Analysis(生物药物分析). Beijing: Chinese University of Medical Sciences and Peking Union Medical College Press(北京：北京医科大学和中国协和医科大学联合出版社), 1998. 14. [9] Steiner T, Koellner G. J. Mol. Biol., 2001, 305(3): 535. [10] LIU Shao-pu, FAN Li, HU Xiao-li, et al(刘绍璞, 范 莉, 胡小莉, 等). Acta Chimica Sinica(化学学报)，2004, 62(17): 1635. [11] Liu Y, Yang J H, Liu S F, et al. Spectrochimica Acta, Part A，2005, 61(4): 641. [12] XIE An-jian, YAO Cheng-li, SHEN Yu-hua(谢安建，姚成立，沈玉华) . Chinese Journal of Inorganic Chemistry(无机化学学报)，2005, 21(3): 413. [13] Umland J B , Bellana J M. General Chemistry. Beijing: China Machine Press(北京: 机械工业出版社), 2004. 431. [14] Tanaka S, Ataka M, Onuma K, et al. Journal of Crystal Growth, Part A, 2002, 237-239: 289.
