Study on the Interaction of Atrazine and ct-DNA by Fluorescence Probe Technique
MA Jing2, MENG Qing-xiang1, REN Li-ping1*, ZHANG Tao2, MU Hao-jie2
1. College of Animal Science and Technology/Beef Cattle Research Center, China Agricultural University, China State Key Laboratory of Animal Nutrition, Beijing 100193, China 2. College of Science, China Agricultural University, Beijing 100193, China
Abstract：The interaction atrazine and ct-DNA was studied using fluorescence probe and UV absorption spectroscopy technique. Ethidium bromide (EB) was used as a probe. The effects of atrazine, phosphate, concentration of ions, and potassium iodide on the fluorescence of ct-DNA-EB system were investigated. The results showed that the fluorescence quenching process of atrazine to ct-DNA-EB system was proved to be the simultaneous dynamic and static quenching model. The phenomena of fluorescence quenching of ct-DNA-atrazine system by KI and hyperchromicity and the red shift of DNA UV-absorption caused by atrazine indicated the existence of intercalation between atrazine and ct-DNA. The experiments of phosphate and ionic strength showed that atrazine could combine with the phosphate group of ct-DNA by non-characteristic static force, and this action could be inhibited by high ionic strength.
马婧2,孟庆翔1,任丽萍1*,张涛2,牟豪杰2. 荧光探针技术研究阿特拉津与ct-DNA的相互作用[J]. 光谱学与光谱分析, 2008, 28(09): 2122-2125.
MA Jing2, MENG Qing-xiang1, REN Li-ping1*, ZHANG Tao2, MU Hao-jie2. Study on the Interaction of Atrazine and ct-DNA by Fluorescence Probe Technique. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2008, 28(09): 2122-2125.
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