| 光谱学与光谱分析 |
|
|
|
|
|
| Studies on the Interaction between Puerarin and Bovine Serum Albumin |
| ZHANG Guo-wen1,2,QUE Qing-min2, PAN Jun-hui2 |
1. The Key Laboratory of Food Science of Ministry of Education, Nanchang University, Nanchang 330047, China
2.Department of Food Science and Engineering, Nanchang University, Nanchang 330047, China |
|
|
|
|
Abstract The interaction of puerarin and bovine serum albumin(BSA) under physiological condition was studied by fluorospectrophotometry. The experiment demonstrated that the quenching mechanism of puerarin a BSA was static quenching process. The quenching constant is 7.29×1012 L·mol -1·s-1, and the binding constant is 5.04×104 L·mol-1. According to the Frster nonradiative energy transfer theory, the binding distance between donor (BSA) and acceptor (puerarin) was calculated to be 3.35 nm. The influence of the presence of puerarin on structure of BSA was studied by synochronous fluorescence method, the binding distance between BSA and puerarin was also measured, and the binding mechanism was discussed. In addition, the effect of some ions on the binding constant of puerarin with BSA was also studied.
|
|
Received: 2006-07-26
Accepted: 2006-10-28
|
|
|
|
Corresponding Authors:
ZHANG Guo-wen
E-mail: gwzhang@ncu.edu.cn
|
|
| Cite this article: |
|
ZHANG Guo-wen,QUE Qing-min,PAN Jun-hui. Studies on the Interaction between Puerarin and Bovine Serum Albumin[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2007, 27(09): 1784-1787.
|
|
|
|
| URL: |
|
https://www.gpxygpfx.com/EN/Y2007/V27/I09/1784 |
[1] Maurice R Eftink, Karen A Hagaman. Biophysical Chemistry, 1985, 22(3): 173.
[2] FENG Xi-zeng, JIN Rui-xiang, QU Yun, et al(冯喜增，金瑞祥，曲 芸，等). Chemical Journal of Chinese Universities(高等学校化学学报)，1996，17(6)：866.
[3] PAN Hong-ping(潘洪平). Guangxi Medical Journal(广西医药), 2003, 25(10): 1941.
[4] HE Hua, YU Jun-sheng, XU Shan-ping, et al(何 华, 于俊生, 徐珊萍, 等). Acta Pharmaceutica Sinica(药学学报), 2001, 36(7): 549.
[5] ZHANG Hai-rong, GUO Si-yuan, LI Lin, et al(张海容, 郭祀远, 李 琳, 等). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2001, 21(6): 829.
[6] YANG Man-man, YANG Pin, ZHANG Li-wei(杨曼曼, 杨 频, 张立伟). Chinese Science Bulletin(科学通报), 1994, 39(1): 31.
[7] CHEN Guo-zhen, HUANG Xian-zhi, ZHENG Zhu-zi, et al(陈国珍, 黄贤智, 郑朱梓，等). Fluoroescence Analysis Method, Second Edition(荧光分析法, 第2版). Beijing: Science Press(北京: 科学出版社), 1990.
[8] GUO Can-cheng, LI He-ping, ZHANG Xiao-bing, et al(郭灿城, 李和平, 张晓兵, 等). Chemical Journal of Chinese Universities(高等学校化学学报), 2003, 24(2): 282.
[9] XIAO Hou-rong, SHENG Liang-quan, SHI Chun-hua, et al(肖厚荣，盛良全，施春华，等). Spectroscopy and Spectral Analysis(光谱学与光谱分析)，2004，24(1)：78.
[10] TANG Bo, DU Ming, CHEN Zhen-zhen, et al(唐 波，杜 鸣，陈蓁蓁，等). Acta Chimica Sinica(化学学报)，2004，62(12)：1153.
[11] YU Tian-zhi, YANG Ru-dong(俞天智, 杨汝栋). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2003, 23(4): 763.
[12] ZHANG Chang-chun, ZHENG Wei-fa, LI Meng-qiu(赵长春, 郑维发, 李梦秋). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2004, 24(1): 111.
[13] LIU Jia-qin, TIAN Jian-niao, BIAN Qing-quan, et al(刘家琴, 田建袅, 边清泉, 等). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2006, 26(4): 715.
[14] LIANG Hong, XING Ben-gang, WU Qing-xuan, et al(梁 宏, 邢本刚, 吴庆轩, 等). Acta Chimica Sinica(化学学报), 1999, 57(5): 161.
[15] QIN Jun-fa, LI Zeng-xi, LOU Man-teng(秦俊法, 李增禧, 楼蔓藤). Guangdong Trace Elements Science(广东微量元素科学), 2005, 12(10): 2. |
| [1] |
YI Min-na1, 2, 3, CAO Hui-min1, 2, 3*, LI Shuang-na-si1, 2, 3, ZHANG Zhu-shan-ying1, 2, 3, ZHU Chun-nan1, 2, 3. A Novel Dual Emission Carbon Point Ratio Fluorescent Probe for Rapid Detection of Lead Ions[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(12): 3788-3793. |
| [2] |
HE Yan-ping, WANG Xin, LI Hao-yang, LI Dong, CHEN Jin-quan, XU Jian-hua*. Room Temperature Synthesis of Polychromatic Tunable Luminescent Carbon Dots and Its Application in Sensitive Detection of Hemoglobin[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(11): 3365-3371. |
| [3] |
LI Jin-zhi1, LIU Chang-jin1, 4*, SHE Zhi-yu2, ZHOU Biao2, XIE Zhi-yong2, ZHANG Jun-bing3, JIANG Shen-hua2, 4*. Antiglycation Activity on LDL of Clove Essential Oil and the Interaction of Its Most Abundant Component—Eugenol With Bovine Serum Albumin[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(01): 324-332. |
| [4] |
SHAO Ke-man, FU Gui-yu, CHEN Su-yan, HONG Cheng-yi, LIN Zheng-zhong*, HUANG Zhi-yong*. Preparation of Molecularly Imprinted Fluorescent Probe for Rare Earth Complex and Determination of Malachite Green Residue[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(03): 808-813. |
| [5] |
WANG Jun1, WANG Zhou-li2, CHENG Jing-jing1. Interaction Between Tartrazine and Bovine Serum Albumin Using Multispectral Method and Molecular Docking[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(03): 904-909. |
| [6] |
WANG Xiao-xia1,3*, WU Hao1, NIE Zhi-hua2, MA Li-tong1,3*, CUI Jin-long1, SAI Hua-zheng1, CHENG Jian-guo1. Study on the Interaction Between Fulvic Acid and Bovine Serum Albumin by Multispectral and Molecular Docking[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2021, 41(09): 2904-2910. |
| [7] |
ZHANG Chuan-ying1, PENG Xin1*, RAO Heng-jun2, QI Wei2, SU Rong-xin2, HE Zhi-min2. Spectroscopic Studies on the Interaction Between Salvianolic Acid B and Bovine Serum Albumin[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2021, 41(06): 1701-1707. |
| [8] |
TUO Xun1, SONG Ji-min1, FU Hao2, LÜ Xiao-lan1*. Study on Interaction Between Hexabromocyclododecane and Bovine Serum Albumin by Spectroscopy and Computer Simulation Methods[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2021, 41(05): 1487-1492. |
| [9] |
LI Xue1, WANG Li1, LIU Guang-xian1*, TU Zong-cai2. Effect of Urea on Glycosylation of BSA Based on Spectral Techniques[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2021, 41(02): 478-483. |
| [10] |
WENG Wen-ting, WANG Si-yu, ZHUANG Jun-yang. Self-Assembled Nanocomposite Film of AgN In-Situ Grown on Polydopamine With Enhanced Fluorescence of CDs for Detection of Puerarin[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2021, 41(01): 168-176. |
| [11] |
LI Xing-xing, ZHANG Xiang, HUANG Xue-song*. Interaction Between Three Sulfur-Containing Amino Acids in Garlic and Bovine Serum Albumin Determined by Fluorescence Spectrometry[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2020, 40(11): 3483-3488. |
| [12] |
JIA Hui-jie, ZHU Ning, GAO Yuan-yuan, WANG Ya-qi, SUO Quan-ling*. Effect of Substituent Structure of Benzothiazole Probe on Recognition to Metal Ion[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2020, 40(11): 3594-3598. |
| [13] |
HUANG Fang1, LIU Ming-xue1, 2*, XIONG Jie1, CHEN Lü-qi1, GAO Zhu-xin1, CHEN Hui-ming1, WANG Dan-ni1. Effect of Far-Infrared Ceramic Powder on the Interaction Between Essential Oil and BSA[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2020, 40(08): 2358-2365. |
| [14] |
WANG Xiao-xia1*, NIE Zhi-hua2, MA Li-tong1, CUI Jin-long1, SAI Hua-zheng1，ZHAO Wen-yuan1. Study on the Interaction Between Minocycline and Bovine Serum Albumin by Multi Spectral Method and Molecular Docking Simulation[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2020, 40(05): 1503-1508. |
| [15] |
LIAN Jie1, REN Yi-fei2, YANG Rui-qin1*, HAO Hong-xia3. Rapid Detection System of 2,4,6-Trinitrophenol (TNP) Based on Fluorescent Probe[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2020, 40(03): 804-808. |
|
|
|
|
