|
|
|
|
|
|
| Effect of Far-Infrared Ceramic Powder on the Interaction Between Essential Oil and BSA |
| HUANG Fang1, LIU Ming-xue1, 2*, XIONG Jie1, CHEN Lü-qi1, GAO Zhu-xin1, CHEN Hui-ming1, WANG Dan-ni1 |
1. School of Life Sciences and Engineering, Southwest University of Science and Technology, Mianyang 621010, China
2. The Center for Co-Operation and Innovation of Nuclear Waste and Environmental Safety and Provincial Department, Mianyang 621010, China |
|
|
|
|
Abstract Essential oil and far-infrared ceramic powder(cFIR) are often used in physiotherapy or related health-care products, but the interaction and mechanism between them and biomolecules are less concerned. In this research, the influence and mechanism of cFIR on the interaction between rose essential oil(REO) and bovine serum albumin (BSA) were studied through different spectroscopy methods and infrared thermography under simulated physiological conditions. The results of the fluorescence spectrum show that both the REO and the cFIR can quench the intrinsic fluorescence of BSA. Whether the cFIR exists or not, the quenching mode of the REO to BSA is static quenching. The fitting calculation of Stern-Volmer equation shows that the binding sites n between BSA and REO is increased from 0.55 to 0.96 by adding cFIR, and the binding constant is also significantly increased, which shows that the affinity of REO to BSA can be improved with the existence of cFIR. The effects of cFIR and REO on the secondary structure of BSA were studied through synchronous fluorescence spectrum, three-dimensional fluorescence spectrum and Fourier transform infrared spectrum. The results show that they can cause the increase of hydrophobicity around the protein and have little effect on the conformation of BSA; in addition, cFIR and REO show the synergistic effect on the quenching of BSA intrinsic fluorescence through the formation of association compounds with BSA. Critical distance (R0) and binding distance (r) were calculated based on Förster’s non-radiative energy transfer theory. The binding distance (r) between BSA and REO changes from 1.445 nm to 1.453 nm after the addition of cFIR, R0 is basically unchanged and R0 is less than r. These results suggest that whether the cFIR exists, the static quenching of BSA fluorescence by REO is accompanied by non-radiative energy transfer. The cFIR mainly improves the affinity of REO to BSA by changing the conformation of BSA due to different non-competitive binding sites between the cFIR and REO on BSA molecular surface. Infrared thermal imaging techniques was used to explore the interaction of REO and cFIR with macromolecules. Infrared thermal imaging results show that REO can reduce the temperature of biomolecules, while cFIR can increase the temperature of biomolecules. The addition of the cFIR can increase a response temperature of BSA-REO system, which indicates the thermal effect will increase (p<0.05). The above results show that the cFIR with nano-size effect and far-infrared radiation can affect the microenvironment of the system by forming association compounds when it interacts with REO, thus affect the fluorescence characteristics and energy transfer of biomolecules. The results of this study can provide a reference for further application of essential oil combined with cFIR in physiotherapy and mechanism research.
|
|
Received: 2019-12-09
Accepted: 2020-04-17
|
|
|
|
Corresponding Authors:
LIU Ming-xue
E-mail: liumingxue@swust.edu.cn
|
|
[1] Vatansever F, Hamblin M R. Photonics Lasers Med., 2012，4: 255.
[2] Tsai S R, Hamblin M R. Journal of Photochemistry and Photobiology, B: Biology, 2017, 170: 197.
[3] Jiang Q D, Wu Y M, Zhang H, et al. Pharmaceutical Biology, 2017, 55(1): 1592.
[4] LI Yun-ping, HE Yun(李云平, 何 云). Laser Journal(激光杂志), 2015, 36(8): 146.
[5] WANG Yu-ting, DENG Pin, LI Hong-juan(王雨婷, 邓 品, 李洪娟). Infrared Technology(红外技术), 2017, 39(1): 14.
[6] WEN Jian, WU Shi-ming(文 建, 吴士明). Laser Magazine(激光杂志), 2019, 40(9): 191.
[7] Mahanthappa M, Savanur M A, Puthusseri B, et al. Biomaterials, 2018, 53: 202.
[8] Roufegarinejad L, Jahanban-Esfahlan A, Sajed-Amin S, et al. Journal of Molecular Recognition, 2018, 31(7): e2704.
[9] Shiri F, Rahimi-Nasrabadi M, Ahmadi F, Ehrlich H. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 2018, 203: 520.
[10] Sekar G, Sugumar S, Mukherjee A, et al. Journal of Luminescence, 2015, 161: 187.
[11] Riaz U, Ashraf S M, Jadoun S, et al. International Journal of Polymeric Materials and Polymeric Biomaterials, 2018, 67: 925.
[12] Campana M, Hosking S L, Petkov J T, et al. Langmuir: the ACS Journal of Surfaces and Colloids, 2015, 31(20): 5614.
[13] Jiang M, Huang C R, Wang Q, et al. Luminescence, 2016, 31(2): 468.
[14] WANG Xiao-xia, NIE Zhi-hua, LI Song-bo, et al(王晓霞, 聂智华, 李松波, 等). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2018, 38(8): 2468.
[15] Zhang Y P, Shi S Y, Liu Y N, et al. Journal of Pharmaceutical and Biomedical Analysis, 2011, 56(5)：1064. |
| [1] |
LEI Hong-jun1, YANG Guang1, PAN Hong-wei1*, WANG Yi-fei1, YI Jun2, WANG Ke-ke2, WANG Guo-hao2, TONG Wen-bin1, SHI Li-li1. Influence of Hydrochemical Ions on Three-Dimensional Fluorescence
Spectrum of Dissolved Organic Matter in the Water Environment
and the Proposed Classification Pretreatment Method[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(01): 134-140. |
| [2] |
GU Yi-lu1, 2，PEI Jing-cheng1, 2*，ZHANG Yu-hui1, 2，YIN Xi-yan1, 2，YU Min-da1, 2, LAI Xiao-jing1, 2. Gemological and Spectral Characterization of Yellowish Green Apatite From Mexico[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(01): 181-187. |
| [3] |
HAN Xue1, 2, LIU Hai1, 2, LIU Jia-wei3, WU Ming-kai1, 2*. Rapid Identification of Inorganic Elements in Understory Soils in
Different Regions of Guizhou Province by X-Ray
Fluorescence Spectrometry[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(01): 225-229. |
| [4] |
WANG Hong-jian1, YU Hai-ye1, GAO Shan-yun1, LI Jin-quan1, LIU Guo-hong1, YU Yue1, LI Xiao-kai1, ZHANG Lei1, ZHANG Xin1, LU Ri-feng2, SUI Yuan-yuan1*. A Model for Predicting Early Spot Disease of Maize Based on Fluorescence Spectral Analysis[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(12): 3710-3718. |
| [5] |
CHENG Hui-zhu1, 2, YANG Wan-qi1, 2, LI Fu-sheng1, 2*, MA Qian1, 2, ZHAO Yan-chun1, 2. Genetic Algorithm Optimized BP Neural Network for Quantitative
Analysis of Soil Heavy Metals in XRF[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(12): 3742-3746. |
| [6] |
SONG Yi-ming1, 2, SHEN Jian1, 2, LIU Chuan-yang1, 2, XIONG Qiu-ran1, 2, CHENG Cheng1, 2, CHAI Yi-di2, WANG Shi-feng2，WU Jing1, 2*. Fluorescence Quantum Yield and Fluorescence Lifetime of Indole, 3-Methylindole and L-Tryptophan[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(12): 3758-3762. |
| [7] |
YANG Ke-li1, 2, PENG Jiao-yu1, 2, DONG Ya-ping1, 2*, LIU Xin1, 2, LI Wu1, 3, LIU Hai-ning1, 3. Spectroscopic Characterization of Dissolved Organic Matter Isolated From Solar Pond[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(12): 3775-3780. |
| [8] |
LI Xiao-li1, WANG Yi-min2*, DENG Sai-wen2, WANG Yi-ya2, LI Song2, BAI Jin-feng1. Application of X-Ray Fluorescence Spectrometry in Geological and
Mineral Analysis for 60 Years[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(10): 2989-2998. |
| [9] |
XUE Fang-jia, YU Jie*, YIN Hang, XIA Qi-yu, SHI Jie-gen, HOU Di-bo, HUANG Ping-jie, ZHANG Guang-xin. A Time Series Double Threshold Method for Pollution Events Detection in Drinking Water Using Three-Dimensional Fluorescence Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(10): 3081-3088. |
| [10] |
MA Qian1, 2, YANG Wan-qi1, 2, LI Fu-sheng1, 2*, CHENG Hui-zhu1, 2, ZHAO Yan-chun1, 2. Research on Classification of Heavy Metal Pb in Honeysuckle Based on XRF and Transfer Learning[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(09): 2729-2733. |
| [11] |
JIA Yu-ge1, YANG Ming-xing1, 2*, YOU Bo-ya1, YU Ke-ye1. Gemological and Spectroscopic Identification Characteristics of Frozen Jelly-Filled Turquoise and Its Raw Material[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(09): 2974-2982. |
| [12] |
YANG Xin1, 2, XIA Min1, 2, YE Yin1, 2*, WANG Jing1, 2. Spatiotemporal Distribution Characteristics of Dissolved Organic Matter Spectrum in the Agricultural Watershed of Dianbu River[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(09): 2983-2988. |
| [13] |
CHEN Wen-jing, XU Nuo, JIAO Zhao-hang, YOU Jia-hua, WANG He, QI Dong-li, FENG Yu*. Study on the Diagnosis of Breast Cancer by Fluorescence Spectrometry Based on Machine Learning[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(08): 2407-2412. |
| [14] |
ZHU Yan-ping1, CUI Chuan-jin1*, CHENG Peng-fei1, 2, PAN Jin-yan1, SU Hao1, 2, ZHANG Yi1. Measurement of Oil Pollutants by Three-Dimensional Fluorescence
Spectroscopy Combined With BP Neural Network and SWATLD[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(08): 2467-2475. |
| [15] |
LIU Xian-yu1, YANG Jiu-chang1, 2, TU Cai1, XU Ya-fen1, XU Chang3, CHEN Quan-li2*. Study on Spectral Characteristics of Scheelite From Xuebaoding, Pingwu County, Sichuan Province, China[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(08): 2550-2556. |
|
|
|
|
