| 光谱学与光谱分析 |
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| Application of Molecular Absorption Spectrophotometric Method to the Determination of Biologic Macromolecular Structures |
| CAO Shu-xia1，ZHAO Yu-fen1, 2* |
1. Department of Chemistry, Zhengzhou University, Zhengzhou 450052，China
2. Department of Chemistry, the Key Laboratory for Bioorganic Phosphorus Chemistry and Chemical Biology, Ministry of Education, School of Life Sciences and Engineering, Tsinghua University, Beijing 100084, China |
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Abstract A review on recent applications of molecular absorption spectrophotometric method to the identification of the structures of biologic macromolecules, such as protein and nucleic acid, is presented. Molecular absorption spectrophotometric method is widely used in the structure determination of biologic macromolecules for its convenience and speed. Ultraviolet absorption spectrum (UV) can be used in the research on the mechanism of the interaction of anticancer drugs and DNA. UV can also be used to study the interaction of spectroscopic probe with biologic molecule and their binding mechanism. Protein secondary structure and conformation can be investigated by Fourier infrared spectroscopy (FTIR) deconvolution analysis. Molecular absorption spectrophotometric method is an important tool for structure study of biologic macromolecules.
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Received: 2003-06-03
Accepted: 2003-10-18
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Corresponding Authors:
ZHAO Yu-fen
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| Cite this article: |
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CAO Shu-xia,ZHAO Yu-fen. Application of Molecular Absorption Spectrophotometric Method to the Determination of Biologic Macromolecular Structures [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2004, 24(10): 1197-1201.
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http://www.gpxygpfx.com/EN/Y2004/V24/I10/1197 |
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