光谱学与光谱分析 |
|
|
|
|
|
Absorption Spectrum Study of HeLa Cells Treated with Vacuum and Low-Energy Ions Implantation |
ZHANG Feng-qiu,HAO Yuan-li,E Xiang-hong,IU Wei,HANG Guang-shui,IN Guang-yong |
School of Physical Engineering, Zhengzhou University, Zhengzhou 450052, China |
|
|
Abstract Mineral oil was selected to protect HeLa cells from water evaporation during low-energy ions implantation in the present paper. Then, HeLa cells having been treated with vacuum and low-energy N+ ions implantation were used to collect ultraviolet absorption spectrum by spectrophotometer. Analytical results indicated that HeLa cells had some characteristic absorption peaks near 202 and 260 nm, respectively. And then the study also found: (1) The spectral intensity increased with the vacuum treatment time. In addition, the effect of vacuum on cellular spectrum was greater than that of mineral oil. (2) The influence of low energy N+ ions on absorption spectrum was far more than that of vacuum. (3) The spectral intensity increased with the implantation dose. According to these results, the effect of low-energy N+ ions implantation and vacuum on tumorous cells (HeLa cells), especially on the molecular configuration and component of tumorous cells (HeLa cells) was discussed. In a word, this study provides a basis for further research on the functionary mechanism of low-energy ions implantation on biomaterial.
|
Received: 2008-08-16
Accepted: 2008-11-18
|
|
Corresponding Authors:
ZHANG Feng-qiu
E-mail: zhangfengqiu@zzu.edu.cn
|
|
[1] ZHANG Feng-qiu, YA Hui-yuan, SU Ming-jie, et al(张凤秋, 押辉远, 苏明杰, 等). Acta Laser Biology Sinica(激光生物学报), 2006, 6(15): 594. [2] ZHANG Fengqiu, YA Huiyuan, LI Jingyuan, et al. Nuclear Science and Techniques, 2006, 4(17): 222. [3] ZHAO Yuan-li, ZHANG Feng-qiu, GE Xiang-hong, et al(赵元黎, 张风秋, 葛向红, 等). Laser Journal(激光杂志), 2007, 2(28): 84. [4] LIANG Er-jun, LIU Yi-zhen, LIU Du-wen, et al(梁二军, 刘一真, 刘杜文, 等). Chinese Journal of Light Scattering(光散射学报), 1999, 11(3): 260. [5] SHAO Chun-lin, BI Qiang, YU Zeng-liang(邵春林, 毕 强, 余增亮). Nuclear Techniques(核技术), 1994, 17(1): 25. [6] Surewicz W K, Mantsch H H. Biochimica et Biophysica Acta, 1998, 952: 115. [7] GUO Rao-jun(郭尧君). Technique of Spectral Photometer and Its Application in Biochemistry(分光光度技术及其在生物化学中的应用). Beijing: Science Press(北京: 科学出版社), 1987. 223. [8] CHANG Jian-hua, DONG Qi-gong(常建华, 董绮功). Spectrum Theory and Resolution(波谱原理及解析). Beijing: Science Press(北京: 科学出版社), 2005. 418. [9] GE Xiang-hong, ZHAO Yuan-li, ZHANG Feng-qiu, et al(葛向红, 赵元黎, 张凤秋, 等). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2004, 24(7): 841. [10] CHEN Guo-zhen(陈国珍). Fluorescence Analyse Means 2rd ed(荧光分析方法, 第2版). Beijing: Science Press(北京: 科学出版社), 1990. 36. [11] WU Sheng-hua(吴升华). Medicine and Philosophy(医学与哲学), 1995, 12(16): 640. [12] GUO Rao-jun(郭尧君). Fluorescence Technique and Application in Molecular Biology(荧光实验技术及其在分子生物学中的应用). Beijing: Science Press(北京: 科学出版社), 1983. 10. [13] GE Xiang-hong, ZHAO Yuan-li, ZHANG Feng-qiu, et al(葛向红, 赵元黎, 张凤秋, 等). Acta Laser Biology Sinica(激光生物学报), 2004, 5(13): 383. [14] YANG Tian-you, LI Zong-wei, ZHANG Feng-qiu, et al(杨天佑, 李宗伟, 张凤秋, 等). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2007, 8(27): 1600.
|
[1] |
ZHENG Pei-chao, YIN Yi-tong, WANG Jin-mei*, ZHOU Chun-yan, ZHANG Li, ZENG Jin-rui, LÜ Qiang. Study on the Method of Detecting Phosphate Ions in Water Based on
Ultraviolet Absorption Spectrum Combined With SPA-ELM Algorithm[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(01): 82-87. |
[2] |
LIU Jia, ZHENG Ya-long, WANG Cheng-bo, YIN Zuo-wei*, PAN Shao-kui. Spectra Characterization of Diaspore-Sapphire From Hotan, Xinjiang[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(01): 176-180. |
[3] |
BAI Xi-lin1, 2, PENG Yue1, 2, ZHANG Xue-dong1, 2, GE Jing1, 2*. Ultrafast Dynamics of CdSe/ZnS Quantum Dots and Quantum
Dot-Acceptor Molecular Complexes[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(01): 56-61. |
[4] |
GU Yi-lu1, 2,PEI Jing-cheng1, 2*,ZHANG Yu-hui1, 2,YIN Xi-yan1, 2,YU Min-da1, 2, LAI Xiao-jing1, 2. Gemological and Spectral Characterization of Yellowish Green Apatite From Mexico[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(01): 181-187. |
[5] |
ZHENG Ni-na1, 2*, XIE Pin-hua1, QIN Min1, DUAN Jun1. Research on the Influence of Lamp Structure of the Combined LED Broadband Light Source on Differential Optical Absorption Spectrum
Retrieval and Its Removing Method[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(11): 3339-3346. |
[6] |
DUAN Ming-xuan1, LI Shi-chun1, 2*, LIU Jia-hui1, WANG Yi1, XIN Wen-hui1, 2, HUA Deng-xin1, 2*, GAO Fei1, 2. Detection of Benzene Concentration by Mid-Infrared Differential
Absorption Lidar[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(11): 3351-3359. |
[7] |
FANG Zheng, WANG Han-bo. Measurement of Plastic Film Thickness Based on X-Ray Absorption
Spectrometry[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(11): 3461-3468. |
[8] |
HUANG Li, MA Rui-jun*, CHEN Yu*, CAI Xiang, YAN Zhen-feng, TANG Hao, LI Yan-fen. Experimental Study on Rapid Detection of Various Organophosphorus Pesticides in Water by UV-Vis Spectroscopy and Parallel Factor Analysis[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(11): 3452-3460. |
[9] |
WANG Peng1, GAO Yong-bao1*, KOU Shao-lei1, MEN Qian-ni1, ZHANG Min1, HE Tao1, YAO Wei2, GAO Rui1, GUO Wen-di1, LIU Chang-rui1. Multi-Objective Optimization of AAS Conditions for Determination of Gold Element Based on Gray Correlation Degree-RSM Model[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(10): 3117-3124. |
[10] |
JIA Yu-ge1, YANG Ming-xing1, 2*, YOU Bo-ya1, YU Ke-ye1. Gemological and Spectroscopic Identification Characteristics of Frozen Jelly-Filled Turquoise and Its Raw Material[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(09): 2974-2982. |
[11] |
YANG Xin1, 2, XIA Min1, 2, YE Yin1, 2*, WANG Jing1, 2. Spatiotemporal Distribution Characteristics of Dissolved Organic Matter Spectrum in the Agricultural Watershed of Dianbu River[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(09): 2983-2988. |
[12] |
CHANG Zhen1, ZHONG Ming-yu2*, SU Jing-ming1, 2, SI Fu-qi1, WANG Yu3, ZHOU Hai-jin1, DOU Ke1, ZHANG Quan1. Study on the Reconstructing the NO2 Gas Distribution in a Vertical Plane Using MAX-DOAS[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(08): 2413-2418. |
[13] |
LIU Xian-yu1, YANG Jiu-chang1, 2, TU Cai1, XU Ya-fen1, XU Chang3, CHEN Quan-li2*. Study on Spectral Characteristics of Scheelite From Xuebaoding, Pingwu County, Sichuan Province, China[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(08): 2550-2556. |
[14] |
TIAN Si-di1, WANG Zhen1, DU Yan-jun2, DING Yan-jun1, PENG Zhi-min1*. High Precision Measurement of Spectroscopic Parameters of CO at 2.3 μm Based on Wavelength Modulation-Direct Absorption Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(07): 2246-2251. |
[15] |
LIANG Wen-ke, WEI Guang-fen, WANG Ming-hao. Research on Methane Detection Error Caused by Lorentzian Profile Approximation[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(06): 1683-1689. |
|
|
|
|