| 光谱学与光谱分析 |
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| Lycopene and β-Carotene Content in Tomato Analyzed by the Second Harmonic |
| OUYANG Shun-li1,2,ZHOU Mi2,CAO Biao2,MEN Zhi-wei2, GAO Shu-qin2,LI Zuo-wei1,2*,LU Guo-hui2， YANG Jian-ge2,3 |
1. State Key Laboratory of Superhard Materials，Jilin University, Changchun 130012, China
2. College of Physics, Jilin University, Changchun 130023, China
3. The Ordnance Engineering Department, Air Force Aviation University, Changchun 130022, China |
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Abstract Lycopene and β-carotene are two important nutritional components in tomato. The main Raman spectrum group of lycopene and β-carotene abundant in tomato is identical and difficult to be distinguished through fundamental frequency. With excitation wavelength of 514.5 nm, the excited light was just present in the half width range of the main absorption bands of Lycopene and β-carotene, so the resonance Raman effect can occur. Based on resonance Raman spectra, by on-body measuring the second harmonic of stretching vibration of carbon-carbon conjugated double bond in lycopene and β-carotene, the content of lycopene and β-carotene can be obtained according to the integrated intensity of each component calculated by software. And this provides a method for on-body determining the content of the components with the homologous group.
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Received: 2008-12-28
Accepted: 2009-04-02
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Corresponding Authors:
LI Zuo-wei
E-mail: zuoweili@jlu.edu.cn
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