| 光谱学与光谱分析 |
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| Spectroscopic Study on Binding of Indomethacin to Bovine Serum Albumin |
| CAO Xi-min,DU Li-ming* |
| Center of Analysis and Test, Shanxi Normal University, Linfen 041004, China |
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Abstract The interaction between indomethacin (IM) and bovine serum albumin (BSA) under physiological condition was studied using fluorescence and absorption spectra. It was proved that fluorescence quenching of BSA by IM is deduced by combining static quenching with nonradiative energy transfer. The binding constants were obtained by fluorescence quenching method. The main sorts of binding force were determined according to the thermodynamic parameters. The binding distances and energy transfer efficiencies between indomethacin and bovine serum albumin were obtained based on the theory of Frster nonradiative energy transfer. Combining the results of UV absorption and fluorescence spectroscopic methods, the binding mode of IM with BSA was discussed.
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Received: 2006-10-12
Accepted: 2007-01-16
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Corresponding Authors:
DU Li-ming
E-mail: liuwzh@dus.sxnu.edu.cn
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| Cite this article: |
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CAO Xi-min,DU Li-ming. Spectroscopic Study on Binding of Indomethacin to Bovine Serum Albumin[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2007, 27(05): 973-977.
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| URL: |
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https://www.gpxygpfx.com/EN/Y2007/V27/I05/973 |
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