| 光谱学与光谱分析 |
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| Study on Spectral Behavior of Meso-Tetra-(3,5-Dibromo-4-Hydroxyphenyl) Porphyrin with Calf Thymus DNA |
| DUAN Cai-hong, CHEN Xin, SUN Shu-ting, MA Hong-min, WANG Jin, DU Bin*, WEI Qin |
| School of Chemistry and Chemical Engineering, University of Jinan, Ji’nan 250022, China |
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Abstract The binding characteristics of meso-tetra-(3,5-dibromo-4-hydroxphenyl) porphyrin [T(DBHP)P] with calf thymus DNA (ct DNA) in pH 4.92 HAc-NaAc buffer solution were studied by the techniques of spectrophotometry. At the maxium absorption wavelength for T(DBHP)P, the decrease in the absorbance was linear with the amount of ct DNA. The experiments indicated that under optimum conditions, ct DNA obeys Beer′s law in the range of 0.20-1.80 μg·mL-1,and the limit of detection is 0.024 μg·mL-1. Tween-80 microemulsion was commended in the experiment for better sensitivity. Furthermore, the association number is also determined by molar ratio method. The interactional mechanism between ct DNA and (T(DBHP)P was investigated as well.
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Received: 2006-12-08
Accepted: 2007-02-18
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Corresponding Authors:
DU Bin
E-mail: sdjndb@263.net
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| Cite this article: |
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DUAN Cai-hong,CHEN Xin,SUN Shu-ting, et al. Study on Spectral Behavior of Meso-Tetra-(3,5-Dibromo-4-Hydroxyphenyl) Porphyrin with Calf Thymus DNA [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2007, 27(05): 969-972.
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https://www.gpxygpfx.com/EN/Y2007/V27/I05/969 |
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