|
|
|
|
|
|
Surface-Enhanced Raman Spectroscopy Analysis on the Serum, Muscular and Synovial Tissue of the Knee in Knee Osteoarthritis Model Rats |
WANG Lu-lu1, LIU Lei1, LI Pan2, WANG Jie3,4, HE Lu3,4, WU Zi-jian3,4*, YANG Liang-bao2, HU Ling4 |
1. School of Post-Graduated Student, Anhui University of Chinese Medicine, Hefei 230012, China
2. Hefei Institute of Physical Science, Chinese Academy of Sciences, Hefei 230031, China
3. School of Acupuncture and Tuina, Anhui University of Chinese Medicine, Hefei 230012, China
4. Institute of Acupuncture and Meridian, Anhui Academy of Chinese Medicine, Hefei 230038, China |
|
|
Abstract Surface-enhanced Raman Spectorscopy (SERS) spectra of serum, knee muscles and synovial tissues of normal rats and Model of Knee Osteoarthritis (KOA) are analyzed, providing an experimental basis for biological changes of KOA. Twenty normal healthy male SD rats were raised under the same conditions and randomly divided into normal control group (referred to as “normal group”) and KOA model group (referred to as “model group”), with 10 rats in each group. The KOA model was prepared by intra-articular injection of 0.03 mol·L-1 of l-cysteine and 4% papain. The classification of surficial enhanced Raman spectra in serum, knee muscle and synovial tissue of rats was detected by silver nanometer substrate fluid. NGLabSpec software was used to compare the differences of Raman frequency shift and characteristic peaks between the two groups, and OriginPro 8.5 software was used to analyze Raman spectra. In the serum, within the range of Raman frequency shift 400~2 000 cm-1, there were 12 characteristic peaks in the normal group and 14 in the model group, and the peak strength of most characteristic peaks in the model group was lower than that in the normal group, and significant difference characteristic peaks appeared in 495, 883 and 1 447 cm-1 of the two groups; in the muscle tissue of the knee joint, there were 12 characteristic peaks in the normal group and 13 in the model group, there were significant differences in the Raman strength of the characteristic peaks of homogeneity between the two groups; in synovial tissues and the normal group has 10 characteristic peaks, has 15 model group, two groups of the common characteristic peak of peak intensity change is not obvious, but in the 655, 950, 1 335, 1 447 cm-1 of the homogeneous characteristic peaks show that the peak strength of significant differences, in 655 and 950 cm-1 of a significant rise in the model group, and 1 335 and 1 447 cm-1 model is the relative strength of two groups decreased significantly. KOA model led to a significant decrease in the number of homogenous characteristic peaks of serum, knee muscle and synovial tissue, and an increase in different substances. Thus the metabolic balance of substances was seriously broken. SERS is a fast and accurate detection method, which can be used for the detection of KOA model.
|
Received: 2019-05-16
Accepted: 2019-10-06
|
|
Corresponding Authors:
WU Zi-jian
E-mail: wuzijian@ahtcm.edu.cn
|
|
[1] LIU Jiao, QU Chong-zheng, XIE Jin-ping, et al(刘 姣, 曲崇正,谢金平, 等). Chinese Journal of Tissue Engineering Research(中国组织工程研究), 2019, 23(19): 3019.
[2] WANG Bing, HU Jing, ZHANG Ning, et al(王 兵, 胡 静, 张 宁, 等). Chinese Acupuncture and Moxibustion(中国针灸), 2017, 37(5): 463,476.
[3] RUAN Li-ping, LIU Jian, YE Wen-fang, et al(阮丽萍, 刘 健, 叶文芳, 等). Rheumaticm and Arthritis(风湿病与关节炎), 2015, 4(3): 5.
[4] WANG Ying-chun, DING Ming, WANG Wei-dong, et al(王迎春, 丁 明, 王卫东, 等). Orthopaedics(骨科), 2019, 10(3): 253.
[5] Zhang W, Sun G, Likhodii S, et al. Osteoarthr Cartil, 2016, 24(5): 827.
[6] XU Bo, MEI Wei, XING Run-lin, et al(徐 波, 梅 伟, 邢润麟, 等). Chinese Archives of Traditional Chinese Medicine(中华中医药学刊), 2018, 36(12): 2987,3100.
[7] LUO Xi, HOU Xue-si, TIAN Zi-yu, et al(罗 溪, 侯学思, 田紫煜, 等). Acupuncture Research(针刺研究), 2019, 44(3): 211.
[8] Vicario A, Sergo V, Togfoli G, et al. Colloids and Surfaces B: Biointerfaces, 2015, 127: 41.
[9] GAO Jia-min, ZHANG Zhuo-min, LI Gong-ke(高嘉敏, 张卓旻, 李攻科). Journal of Instrumental Analysis(分析测试学报), 2016, 35(12): 1647.
[10] Ma Bingbing, Li Pan, Yang Liangbao, et al. Talanta, 2015, 141: 1.
[11] WANG Xiao-hui, XU Tao-tao, HUANG Yi-qun, et al(王晓辉, 徐涛涛, 黄轶群, 等). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2019, 39(1): 123.
[12] Reuter C W M, Morgan M A, Eckardt A. British Journal of Cancer, 2007, 96(3): 408.
[13] LIU Wen-yan, PAN Qing, WANG Ke, et al(刘文艳, 潘 清, 王 珂, 等). China Medical Equipment(中国医学装备), 2015, 12(1): 81.
[14] Corina D Bocsa, Vlad Moisoiu MSc, Andrei Stefancu BSc, et al. Nanomedicine,2019, 4: 15.
[15] DUAN Wen-xiu, WANG Zong-bao, ZHANG Hao, et al(段文秀, 汪宗保, 张 浩, 等). Chinese Journal of Tissue Engineering Research(中国组织工程研究), 2015, 19(18): 2789. |
[1] |
XING Hai-bo1, ZHENG Bo-wen1, LI Xin-yue1, HUANG Bo-tao2, XIANG Xiao2, HU Xiao-jun1*. Colorimetric and SERS Dual-Channel Sensing Detection of Pyrene in
Water[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(01): 95-102. |
[2] |
LU Wen-jing, FANG Ya-ping, LIN Tai-feng, WANG Hui-qin, ZHENG Da-wei, ZHANG Ping*. Rapid Identification of the Raman Phenotypes of Breast Cancer Cell
Derived Exosomes and the Relationship With Maternal Cells[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(12): 3840-3846. |
[3] |
GUO He-yuanxi1, LI Li-jun1*, FENG Jun1, 2*, LIN Xin1, LI Rui1. A SERS-Aptsensor for Detection of Chloramphenicol Based on DNA Hybridization Indicator and Silver Nanorod Array Chip[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(11): 3445-3451. |
[4] |
LI Wen-wen1, 2, LONG Chang-jiang1, 2, 4*, LI Shan-jun1, 2, 3, 4, CHEN Hong1, 2, 4. Detection of Mixed Pesticide Residues of Prochloraz and Imazalil in
Citrus Epidermis by Surface Enhanced Raman Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(10): 3052-3058. |
[5] |
ZHAO Ling-yi1, 2, YANG Xi3, WEI Yi4, YANG Rui-qin1, 2*, ZHAO Qian4, ZHANG Hong-wen4, CAI Wei-ping4. SERS Detection and Efficient Identification of Heroin and Its Metabolites Based on Au/SiO2 Composite Nanosphere Array[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(10): 3150-3157. |
[6] |
SU Xin-yue1, MA Yan-li2, ZHAI Chen3, LI Yan-lei4, MA Qian-yun1, SUN Jian-feng1, WANG Wen-xiu1*. Research Progress of Surface Enhanced Raman Spectroscopy in Quality and Safety Detection of Liquid Food[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(09): 2657-2666. |
[7] |
YANG Jing1, LI Li1, LIANG Jian-dan1, HUANG Shan1, SU Wei1, WEI Ya-shu2, WEI Liang1*, XIAO Qi1*. Study on the Interaction Mechanism Between Thiosemicarbazide Aryl Ruthenium Complexes and Human Serum Albumin[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(09): 2761-2767. |
[8] |
ZHAO Yu-wen1, ZHANG Ze-shuai1, ZHU Xiao-ying1, WANG Hai-xia1, 2*, LI Zheng1, 2, LU Hong-wei3, XI Meng3. Application Strategies of Surface-Enhanced Raman Spectroscopy in Simultaneous Detection of Multiple Pathogens[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(07): 2012-2018. |
[9] |
TANG Yan1, YANG Yun-fan1, HU Jian-bo1, 2, ZHANG Hang2, LIU Yong-gang3*, LIU Qiang-qiang4. Study on the Kinetic Process and Spectral Properties of the Binding of Warfarin to Human Serum Protein[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(07): 2099-2104. |
[10] |
CHENG Chang-hong1, XUE Chang-guo1*, XIA De-bin2, TENG Yan-hua1, XIE A-tian1. Preparation of Organic Semiconductor-Silver Nanoparticles Composite Substrate and Its Application in Surface Enhanced Raman Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(07): 2158-2165. |
[11] |
LI Chun-ying1, WANG Hong-yi1, LI Yong-chun1, LI Jing1, CHEN Gao-le2, FAN Yu-xia2*. Application Progress of Surface-Enhanced Raman Spectroscopy for
Detection Veterinary Drug Residues in Animal-Derived Food[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(06): 1667-1675. |
[12] |
HUANG Xiao-wei1, ZHANG Ning1, LI Zhi-hua1, SHI Ji-yong1, SUN Yue1, ZHANG Xin-ai1, ZOU Xiao-bo1, 2*. Detection of Carbendazim Residue in Apple Using Surface-Enhanced Raman Scattering Labeling Immunoassay[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(05): 1478-1484. |
[13] |
LU Yan-hua, XU Min-min, YAO Jian-lin*. Preparation and Photoelectrocatalytic Properties Study of TiO2-Ag
Nanocomposites[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(04): 1112-1116. |
[14] |
WANG Yi-tao1, WU Cheng-zhao1, HU Dong1, SUN Tong1, 2*. Research Progress of Plasticizer Detection Based on Raman Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(04): 1298-1305. |
[15] |
REN Li-lei, PENG Yu-ling, WANG Shu-jun*, ZHANG Cheng-gen, CHEN Yu, WANG Xin-tong, MENG Xiao-ning. Fluorescence Spectroscopy for Studies on the Interaction Between Three Metalloporphyrins With Human Serum Albumin[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(03): 806-813. |
|
|
|
|