光谱学与光谱分析 |
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Spectroscopic Study on the Conformational Change of Silk Fibroin in Methanol-Water Mixtures |
MA Lin,HE Wei-ren,HUANG Ai-min,LI Li-shuo,TONG Zhang-fa, WEI Qiao-na,HUANG Zi-lun |
College of Chemistry and Chemical Engineering,Guangxi University,Nanning 530004,China |
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Abstract A combinational study of circular dichroism,intrinsic fluorescence of protein and exogenous fluorescence probe of ANS was made to investigate the conformational change of silk fibroin in methanol-water mixtures as well as the mechanism.The spectral results showed that small hydrophobic regions were formed in silk fibroin in methanol-water mixtures at the concentration lower than 30% (V/V) via hydrophobic interaction,which was decreased at higher methanol content due to a structural transition of silk fibroin from random coil to β-sheet.The conformational change of silk fibroin was found to be of a close relationship with the microstructure of the solvent and to be determined by the interaction between the peptide unit of silk fibroin and the cluster of the mixed solvent.Methanol-water mixture at low concentration had little effect on the solvation of the peptide unit and the conformation of silk fibroin,as a consequence of the fact that the inherent water structure was conserved.The transition from the tetrahedral-like water structure to the chain-like methanol structure,due to the increasing concentration of methanol,induced the conformational change of silk fibroin to eliminate the contact of peptide unit with the solvent molecular.
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Received: 2009-12-16
Accepted: 2010-03-22
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Corresponding Authors:
MA Lin
E-mail: malinzju@gxu.edu.com
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