| 光谱学与光谱分析 |
|
|
|
|
|
| Fluorescence Study on the Interaction of Alizarin Yellow R and Bovine Serum Albumin in Alkali Solution |
| ZHANG Huan1, WANG Xing-ming1*, WANG Qing-cheng1, SHI Rong-ming1, DING Li-sheng2 |
1. Institute of Chemistry，Southwest University of Science and Technology, Mianyang 621010, China
2. Chengdu Institute of Biology， Chinese Academy of Sciences， Chengdu 610041, China |
|
|
|
|
Abstract The interaction of alizarin yellow R(AYR) and bovine serum albumin (BSA)was investigated by fluorescence method in alkali buffer solution. It was shown that AYR had a powerful ability to quench the BSA fluorescence at excitation and emission wavelengths of λex=393 nm and λem=641 nm in the medium solution of pH 11.00, and there were five binding sites of AYR to BSA;The combination reaction of AYR with BSA was a static quenching process, and from the effects of temperature on the fluorescence quenching rate of AYR-BSA and the Stern-Volmer quenching constant (KSV) and the Lineweaver-Burk quenching constant (KLB), the binding constant was calculated to be K=1.6×104 L·mol-1;as the enthalpy change ΔHθ＜0 and entropy change ΔSθ＜0, and AYR has an ability to quench the BSA-CBBG fluorescence, it can be deduced that the Van der Walls force and hydrogen bond are the main binding forces between AYR and BSA.
|
|
Received: 2005-11-01
Accepted: 2006-02-09
|
|
|
|
Corresponding Authors:
WANG Xing-ming
|
|
| Cite this article: |
|
ZHANG Huan,WANG Xing-ming,WANG Qing-cheng, et al. Fluorescence Study on the Interaction of Alizarin Yellow R and Bovine Serum Albumin in Alkali Solution[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2006, 26(08): 1508-1512.
|
|
|
|
| URL: |
|
https://www.gpxygpfx.com/EN/Y2006/V26/I08/1508 |
[1] HUANG Kai-xun, LIU Qiong, YANG Xiang-liang, et al(黄开勋, 刘 琼, 杨祥良, 等). Progress in Chemistry(化学进展), 2004, 16(2): 308.
[2] YAN Cheng-nong，SHANGGUAN Yun-feng，TONG Jin-qiang, et al(颜承农, 上官云凤, 童金强, 等). Spectroscopy and Spectral Analysis(光谱学与光谱分析)，2003，23(3)：543.
[3] Erkens J H F, Dieleman S J, Dressendorfer R A. Journal of Steroid Biochem. Molec. Biol.，1998，67(2): 153.
[4] Hu Shen, Zhang Le, Norman J. Journal of Chromatography A, 2001, 924: 369.
[5] CHEN Guo-zhen, HUANG Xian-zhi, ZHENG Zhu-zi, et al(陈国珍, 黄贤智, 郑朱梓, 等). Fluorescence Analytical Methods(荧光分析法). Beijing: Science Press(北京: 科学出版社), 1990. 112.
[6] Penzer G R. Translated by ZHU Da-chang, CHEN Jian, ZHU Shi-sheng(祝大昌, 陈 剑，朱世盛译). Molecular Luminescence Analytical Methods(Fluorescence and Phosphorescence Methods)(分子发光分析法·荧光和磷光法). Shanghai: Fudan University Press(上海: 复旦大学出版社)，1985. 17.
[7] Mudd J B, Dawson P J, Tseng Sam. Archives of Biochemistry and Biophysics，1997, 338(2): 338.
[8] Joan Q Zhong, Yelena Freyzon, Daniel J. Biomolecular Engineering, 2004, 21：67.
[9] YANG Man-man，YANG Pin，ZHANG Li-wei(杨曼曼, 杨 频, 张立伟). Chinese Science Bulletin(科学通报), 1994, 39(1): 31.
[10] XIAO Hou-rong, SHENG Liang-quan, SHI Chun-hua, et al(肖厚荣, 盛良全, 施春华, 等). Spectroscopy and Spectral Analysis(光谱学与光谱分析)，2004，24(1)：78.
[11] Ross D P, Subramanian S. Biochemistry, 1981, 20: 3096.
[12] CUI Feng-lin, FAN Jing, LI Jian-ping. Bioorganic Medicinal Chemistry, 2004, 12: 151.
[13] GAO Zhi-yu，XUE Min-zhao，LIU Yan-gang, et al(高志宇, 薛敏钊, 刘燕刚, 等). Photographic Science and Photochemistry(感光科学与光化学)，2002，20(4)：269.
[14] ZHOU Xian-wan, HU Xiao-qian(周先碗，胡晓倩). Instrumental Analysis and Experimental Technology of Biochemistry(生物化学仪器分析与实验技术). Beijing: Chemical Industry Press(北京: 化学工业出版社)，2002. 24.
|
| [1] |
LEI Hong-jun1, YANG Guang1, PAN Hong-wei1*, WANG Yi-fei1, YI Jun2, WANG Ke-ke2, WANG Guo-hao2, TONG Wen-bin1, SHI Li-li1. Influence of Hydrochemical Ions on Three-Dimensional Fluorescence
Spectrum of Dissolved Organic Matter in the Water Environment
and the Proposed Classification Pretreatment Method[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(01): 134-140. |
| [2] |
GU Yi-lu1, 2，PEI Jing-cheng1, 2*，ZHANG Yu-hui1, 2，YIN Xi-yan1, 2，YU Min-da1, 2, LAI Xiao-jing1, 2. Gemological and Spectral Characterization of Yellowish Green Apatite From Mexico[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(01): 181-187. |
| [3] |
HAN Xue1, 2, LIU Hai1, 2, LIU Jia-wei3, WU Ming-kai1, 2*. Rapid Identification of Inorganic Elements in Understory Soils in
Different Regions of Guizhou Province by X-Ray
Fluorescence Spectrometry[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(01): 225-229. |
| [4] |
WANG Hong-jian1, YU Hai-ye1, GAO Shan-yun1, LI Jin-quan1, LIU Guo-hong1, YU Yue1, LI Xiao-kai1, ZHANG Lei1, ZHANG Xin1, LU Ri-feng2, SUI Yuan-yuan1*. A Model for Predicting Early Spot Disease of Maize Based on Fluorescence Spectral Analysis[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(12): 3710-3718. |
| [5] |
CHENG Hui-zhu1, 2, YANG Wan-qi1, 2, LI Fu-sheng1, 2*, MA Qian1, 2, ZHAO Yan-chun1, 2. Genetic Algorithm Optimized BP Neural Network for Quantitative
Analysis of Soil Heavy Metals in XRF[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(12): 3742-3746. |
| [6] |
SONG Yi-ming1, 2, SHEN Jian1, 2, LIU Chuan-yang1, 2, XIONG Qiu-ran1, 2, CHENG Cheng1, 2, CHAI Yi-di2, WANG Shi-feng2，WU Jing1, 2*. Fluorescence Quantum Yield and Fluorescence Lifetime of Indole, 3-Methylindole and L-Tryptophan[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(12): 3758-3762. |
| [7] |
YANG Ke-li1, 2, PENG Jiao-yu1, 2, DONG Ya-ping1, 2*, LIU Xin1, 2, LI Wu1, 3, LIU Hai-ning1, 3. Spectroscopic Characterization of Dissolved Organic Matter Isolated From Solar Pond[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(12): 3775-3780. |
| [8] |
LI Xiao-li1, WANG Yi-min2*, DENG Sai-wen2, WANG Yi-ya2, LI Song2, BAI Jin-feng1. Application of X-Ray Fluorescence Spectrometry in Geological and
Mineral Analysis for 60 Years[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(10): 2989-2998. |
| [9] |
XUE Fang-jia, YU Jie*, YIN Hang, XIA Qi-yu, SHI Jie-gen, HOU Di-bo, HUANG Ping-jie, ZHANG Guang-xin. A Time Series Double Threshold Method for Pollution Events Detection in Drinking Water Using Three-Dimensional Fluorescence Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(10): 3081-3088. |
| [10] |
MA Qian1, 2, YANG Wan-qi1, 2, LI Fu-sheng1, 2*, CHENG Hui-zhu1, 2, ZHAO Yan-chun1, 2. Research on Classification of Heavy Metal Pb in Honeysuckle Based on XRF and Transfer Learning[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(09): 2729-2733. |
| [11] |
JIA Yu-ge1, YANG Ming-xing1, 2*, YOU Bo-ya1, YU Ke-ye1. Gemological and Spectroscopic Identification Characteristics of Frozen Jelly-Filled Turquoise and Its Raw Material[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(09): 2974-2982. |
| [12] |
YANG Xin1, 2, XIA Min1, 2, YE Yin1, 2*, WANG Jing1, 2. Spatiotemporal Distribution Characteristics of Dissolved Organic Matter Spectrum in the Agricultural Watershed of Dianbu River[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(09): 2983-2988. |
| [13] |
CHEN Wen-jing, XU Nuo, JIAO Zhao-hang, YOU Jia-hua, WANG He, QI Dong-li, FENG Yu*. Study on the Diagnosis of Breast Cancer by Fluorescence Spectrometry Based on Machine Learning[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(08): 2407-2412. |
| [14] |
ZHU Yan-ping1, CUI Chuan-jin1*, CHENG Peng-fei1, 2, PAN Jin-yan1, SU Hao1, 2, ZHANG Yi1. Measurement of Oil Pollutants by Three-Dimensional Fluorescence
Spectroscopy Combined With BP Neural Network and SWATLD[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(08): 2467-2475. |
| [15] |
LIU Xian-yu1, YANG Jiu-chang1, 2, TU Cai1, XU Ya-fen1, XU Chang3, CHEN Quan-li2*. Study on Spectral Characteristics of Scheelite From Xuebaoding, Pingwu County, Sichuan Province, China[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(08): 2550-2556. |
|
|
|
|
