| 光谱学与光谱分析 |
|
|
|
|
|
| Examining the Effects of Biochar Application on Soil Phosphorus Levels and Phosphatase Activities with Visible and Fluorescence Spectroscopy |
| ZHANG Yu-lan1, CHEN Li-jun1*, ZHANG Yu-ge2, WU Zhi-jie1, MA Xing-zhu3, YANG Xiao-zhu1 |
1. Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China
2. Key Laboratory of Regional Environment and Eco-remediation, College of Environment, Shenyang University, Shenyang 110044, China
3. Institute of Soil Fertilizer and Environment Resource, Heilongjiang Academy of Agricultural Sciences, Harbin 150086, China |
|
|
|
|
Abstract Biochar could be got from crop straw which contain rich carbon under oxygen free or oxygen limited conditions at low temperature. The application of biochar into soil is beneficial to ease the pressure of handling straw, reduce pollution, reduce greenhouse gas emissions and improve soil quality. This study was carried out in a cornfield containing meadow brown soil at the lower reaches of Liao River which was treated with different amounts of biochar (0, 360, 1 800, 3 600 kg·ha-1) and fertilizer. We investigated the contents of soil available phosphorus (AP), organic P (OP) and total P (TP). We also investigated the enzyme activities of soil acid phosphatase (AcP), alkaline phosphatase (AlP) and phosphodiesterase (PD) via a fluorescence spectroscopy method by using a fluorescent conjugated polymer as the substrate. Soil AP contents increased drastically with the increasing application of biochar, whereas the OP and TP contents exhibited little change. The increase in AP contents was ascribed to the introduction of P into the soil via biochar. Soil AlP and PD activities increased with increasing biochar application. Soil AcP activity increased significantly after the application of the appropriate amount of biochar (1 800 kg·ha-1), whereas it was inhibited by the application of high levels of biochar (3 600 kg·ha-1), perhaps due to the intrinsic alkalinity of biochar. The effect of Biochar inputs on soil phosphorus element and phosphatase activity is the comprehensive embodiment of the soil physical properties, chemical properties, and microbial community structure and metabolic capacity. We should further study such item. The fluorescent microplate method used in this study has many advantages, such as accuracy, rapidness and simple to perform.
|
|
Received: 2015-02-10
Accepted: 2015-06-08
|
|
|
|
Corresponding Authors:
CHEN Li-jun
E-mail: ljchenchina@hotmail.com;ljchen@iae.ac.cn
|
|
[1] Beck MA，Sanchez PA. Plant and Soil, 1996, 184(1): 23.
[2] Chen W F, Zhang W M, Meng J, et al. Engineering Sciences, 2011, 9(2): 83.
[3] Streubel J D, Collins H P, Garcia-Perez M, et al. Soil Science Society of America Journal, 2011, 75(4): 1402.
[4] Angst T E, Sohi S P. GCB Bioenergy, 2013, 5(2):221.
[5] Laird D A, Fleming P, Davis D D, et al. Geoderma, 2010, 158(3): 443.
[6] Chintala R, Schumacher T E, McDonald L M, et al. Clean-Soil, Air, Water, 2014, 42(5): 626.
[7] Yao Y, Gao B, Zhang M, et al. Chemosphere, 2012, 89(11):1467.
[8] Hale S E, Alling V, Martinsen V, et al. Chemosphere, 2013, 9: 1612.
[9] Wei K, Chen Z H, Zhang X P, et al. Geoderma，2014, 217-218: 37.
[10] Muhittin O A, Ayten N. European Journal of Soil Science，in press, 2015.
[11] Zhang Y L, Chen L J, Duan Z H, et al. Spectrosc. Spectr. Anal.，2014, 34(2): 455.
[12] Kuo S. In: Methods of Soil Analysis, Part 3, Chemical Methods (Sparks, D. L., et al., eds.), SSSA Book series No 5. Soil Science of America, Madison, WI, 1996. 869.
[13] DeLuca T H, MacKenzie M D, Gundale M J. In: Biochar for Environmental Management: Science and Technology. London, UK: Earthscan，2009. 251.
[14] Galvez A, Sinicco T, Cayuela M I, et al. Agriculture, Ecosystems and Enviroment. 2012, 160: 3.
[15] Masto RE, Kumar S, Rout T K, et al. Catena，2013, 111: 64. |
| [1] |
LI Qi-chen1, 2, LI Min-zan1, 2*, YANG Wei2, 3, SUN Hong2, 3, ZHANG Yao1, 3. Quantitative Analysis of Water-Soluble Phosphorous Based on Raman
Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(12): 3871-3876. |
| [2] |
CHEN Wei1, SUN Cong-jian1, LI Wei-hong2*. Fluorescence Spectrometric Determination of Cellulase in the rhizosphere of Buckwheat Soil under Low Nitrogen Stress[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(10): 3159-3162. |
| [3] |
YANG Xiao-zhu1,2, CHEN Zhen-hua1, ZHANG Yu-lan1, CHEN Li-jun1* . Effect of Exogenous Phytase Addition on Soil Phosphatase Activities: a Fluorescence Spectroscopy Study[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2015, 35(05): 1294-1299. |
| [4] |
ZHANG Yu-lan1, CHEN Li-jun1*, DUAN Zheng-hu2, WU Zhi-jie1, SUN Cai-xia3, WANG Jun-yu1 . Change in Soil Enzymes Activities after Adding Biochar or Straw by Fluorescent Microplate Method[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2014, 34(02): 455-459. |
| [5] |
XU Liang-yuan1, 2，LIU Yong2，ZHANG Gong3，ZHU Ling2，WANG Yi-kun2，KONG Xiao-ling1，WU Lu-sheng2 . Technology of Advanced Glycation Endproducts Fluorescence Measurement and Precorrection[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2010, 30(01): 230-232. |
|
|
|
|
