| 光谱学与光谱分析 |
|
|
|
|
|
| Technology of Advanced Glycation Endproducts Fluorescence Measurement and Precorrection |
| XU Liang-yuan1, 2，LIU Yong2，ZHANG Gong3，ZHU Ling2，WANG Yi-kun2，KONG Xiao-ling1，WU Lu-sheng2 |
1.Engineering College，Anhui Agricultural University，Hefei 230036，China
2.Anhui Institute of Optics and Fine Mechanics，Chinese Academy of Sciences，Hefei 230031，China
3.University of Winnipeg，Manitoba，R3T6A5，Canada |
|
|
|
|
Abstract The necessity to measure advanced glycation endproducts (AGE) was analyzed in the present paper, and the apparatus for measuring skin autofluorescence was also designed making use of the special excitation spectrum and emission spectrum.A portion of tissue at the volar side of the arm of individuals (11 diabetes and 19 control subjects) was illuminated with excitation light, i.e.monochromatic light around 370 nm, and the emission spectrum was detected on the skin of control subjects and diabetic patients respectively.All measurements were performed at room temperature in a semi-dark environment.It can be seen that different sites of an individual could lead to different results, and the color can also affect the results.The technology of fluorescence precorrection was applied in order to get rid of the influence of noise, different site of skin, the color of skin etc.The result indicates that the technology of precorrection is of avail and the repetitiveness is well.
|
|
Received: 2009-02-08
Accepted: 2009-05-12
|
|
|
|
Corresponding Authors:
XU Liang-yuan
E-mail: xlyjwh@126.com
|
|
[1] SUN Mian-en, DU Guan-hua(孙缅恩, 杜冠华).Chinese Pharmacological Bulletin(中国药理学通报), 2002, 18(3): 246.
[2] Michael Brownlee.Annu.Rev.Med., 1991, 42: 159.
[3] Miyata T, Van Ypersele de Strihou C, Kurokawa H, et al.Kidney Int., 1999, 55: 389.
[4] Dukic-Stefanovic S, Schinxel R, Reiderer P, et al.Biogerontology, 2001, 2: 19.
[5] Lyons U, Silvestri G, Dunn J A, et al.Diabetes, 1991, 40(8): 1010.
[6] Kikuchi S, Shinpo K, Ogata A, et al.Amyotroph Lateral Sclerosis and Other Motor Neuron Disorders, 2002, 3(2): 63.
[7] Fumitaka Hayase, Ramanakoppa H Nagaraj, Satoshi Miyata, et al.Monnier.Biological Chemistry, 1989, 264(7): 3758.
[8] Mulder D J, Water T, Lutgers H L, et al.Diabetes Technology & Therapeutics, 2006, 8(5): 523.
[9] Daniel G Dyer, John A Dunn, Suzanne R Thorpe, et al.Bailie.J.Clin.Invest., 1993, 91(6): 2463.
[10] ThomeJ, Munch G, MUlier R, et al.Life Sci., 1996, 59(8): 679.
[11] Wu J T, Tu M C, Zhung P.J.Clin.Lab.Anal., 1996, 10(1): 21.
[12] Münch G, Keis R, Wessels A, et al.Eur.J.Clin.Chem.Clin.Biochem., 1997, 35(9): 669.
[13] McCance D R, Dyer D G, Dunn J A, et al.J.Clin.Invest., 1993, 91(6): 2470.
[14] LI Bu-hong, ZHANG Zhen-xi, XIE Shu-sen, et al(李步洪, 张镇西, 谢树森, 等).Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2006, 26(7): 1310.
|
| [1] |
XIE Xue-wei2, ZHONG Hao-chen2, CHEN Zhen-cheng2, HE Min2, ZHU Jian-ming1*. Detection of Advanced Glycosylation End Products by Fluorescence Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2021, 41(04): 1055-1059. |
| [2] |
CHEN Wei1, SUN Cong-jian1, LI Wei-hong2*. Fluorescence Spectrometric Determination of Cellulase in the rhizosphere of Buckwheat Soil under Low Nitrogen Stress[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(10): 3159-3162. |
| [3] |
ZHANG Yu-lan1, CHEN Li-jun1*, ZHANG Yu-ge2, WU Zhi-jie1, MA Xing-zhu3, YANG Xiao-zhu1. Examining the Effects of Biochar Application on Soil Phosphorus Levels and Phosphatase Activities with Visible and Fluorescence Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2016, 36(07): 2325-2329. |
| [4] |
LI Fei1, WANG Yi-kun1, 2, ZHU Ling1, 2, ZHANG Yuan-zhi1, JI Min1, ZHANG Long1, LIU Yong1, 2*, WANG An1 . Research on Noninvasive Risk Evaluation of Diabetes Mellitus Based on Neural Network Pattern Recognition [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2014, 34(05): 1327-1331. |
| [5] |
ZHANG Yu-lan1, CHEN Li-jun1*, DUAN Zheng-hu2, WU Zhi-jie1, SUN Cai-xia3, WANG Jun-yu1 . Change in Soil Enzymes Activities after Adding Biochar or Straw by Fluorescent Microplate Method[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2014, 34(02): 455-459. |
| [6] |
XU Liang-yuan1, 2, ZHU Ling1, ZHANG Long1, YU Feng3, LIU Yong1, ZHANG Gong4, WU Lu-sheng1 . Design of Advanced Glycation Endproducts Fluorescence Spectrum Detecting System[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2009, 29(08): 2298-2301. |
|
|
|
|
