Determination of Amorphous Iron Oxides in Soil by Hydroxylamine Extraction-Spectrophotometry
CHI Guang-yu1,2, ZHANG Zhao-wei3, CHEN Xin1*, SHI Yi1
1. Key Laboratory of Terrestrial Ecological Process, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China 2. Graduate School of Chinese Academy of Sciences, Beijing 100039, China 3. Shenyang Agricultural University, Shenyang 110161, China
Abstract：Amorphous iron oxides in soil were determined by hydroxylamine extraction-spectrophotometry. The results showed that hydroxylamine extraction eliminates interference of magnetite and the defect of results being on the high side was overcome to a certain degree compared with oxalic acid-oxalic acid ammonium extraction. The hydroxylamine extraction-spectrophotometry for the detection of amorphous iron oxides in soil was highly precise (relative standard deviation was less than 2.0%) and highly reliable (recovery rates ranged from 97.5％ to 101.5％). Other advantages of the method were rapidness，simplicity and a shorter chromogenic time. In addition, soil incubated anaerobically at constant temperature under laboratory condition was investigated. The results suggested that iron reduction rates during initiation, rapid reaction and steady phases in the soil samples anaerobic incubation were 0.030-0.053, 0.186-0.333 and 0.015-0.030 g·d-1·kg-1, respectively. Significant relationship between the concentrations of hydroxylamine extraction iron and iron reduction rates during rapid reaction phase in soil incubation (r=0.990 7, p＜0.01) indicated that hydroxylamine had a higher degree of selectivity in amorphous iron oxides extraction than oxalic acid-oxalic acid ammonium extraction.
Key words：Spectrophotometry;Amorphous iron oxides in soil;Oxalic acid-oxalic acid ammonium extraction;Hydroxylamine extraction
迟光宇1,2,张兆伟3,陈欣1*,史奕1 . 羟胺浸提-可见分光光度法测定土壤无定形铁[J]. 光谱学与光谱分析, 2008, 28(12): 2931-2934.
CHI Guang-yu1,2, ZHANG Zhao-wei3, CHEN Xin1*, SHI Yi1. Determination of Amorphous Iron Oxides in Soil by Hydroxylamine Extraction-Spectrophotometry. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2008, 28(12): 2931-2934.
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