Determination of Physiological Indices in Albizzia Julibrissin Durazz Seedlings under Alkaline Stress with Visible Spectrophotometry
ZHOU Jian1,ZHANG Lin2,YUAN De-yi2,QI An-guo1
1. Botanical Garden Institute, Henan Institute of Science and Technology, Xinxiang 453003, China 2. The Key Lab of Non-wood Forest Product of Forestry Ministry, Central South University of Forestry and Technology, Changsha 410004, China
Abstract：There is a large area of saline-alkali soil in our country, and soil alkalization is always a problem affecting urban gardening. To examine the capacity of alkaline resistance of Albizzia julibrissin Durazz seedlings, the contents of MDA, soluble sugar and proline, and the activity of POD and SOD in Albizzia julibrissin durazz tree body were measured by means of visible spectrophotometry. Also, the change patterns of the five indexes with different treatment concentration and time were analyzed. Attempts were then made to elucidate the physiological mechanism of how alkaline stress affects the growth of the Albizzia julibrissin durazz tree, which could provide theoretical foundation for planting and gardening and an approach to dealing with the difficulties in planting and gardening in saline and alkaline area. The results showed that with the increase in Na2CO3 concentration, the contents of MDA and soluble sugar in the leaves slowly ascended when the treatment concentration was lower than 75 mmol·L-1,and then rapidly increased when the treatment concentration was higher than 75 mmol·L-1; There were significant differences between different treatments. Proline content exhibited the same change pattern with MDA and soluble sugar. It slowly ascended when the treatment concentration was lower than 100 mmol·L-1,whereas it sharply increased when the treatment concentration was above 100 mmol·L-1; The changes in SOD and POD were similar, showing a unimodal pattern. However, the treatment concentration corresponding to the maximum of SOD and POD was 50 and 75 mmol·L-1,respectively. With the changes in stress time, in addition, the contents of MDA treated with the same concentration increased gradually. However, praline, soluble sugar, SOD and POD changed irregularly. These results indicated that Albizzia julibrissin Durazz could resist the alkaline stress by modulating values of physical indexes such as the contents of MDA, soluble sugar and proline and the activity of POD and SOD.
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