Study on the Content Determination of Total Flavonoids in Olea europaea L. Leaves
ZHENG Yuan-yuan1, 2, LI Chen2, 3, FENG Shi-lan1, HUANG Xin-yi2, LIU Yong-feng2, 4, CHEN Xiao-fen2, 4, DI Duo-long1，2*
1. Lanzhou University Pharmaceutical College, Lanzhou 730000, China 2. Key Laboratory of Chemistry of Northwestern Plant Resources & Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, China 3. Instrumental Analysis & Research Center, School of Applied Physics & Material Engineering, Wuyi University, Jiangmen 529020, China 4. Graduate University of Chinese Academy of Sciences, Beijing 100049, China
Abstract：A quantitative determination method of the total flavonoids in Olea europaea L. leaves using rutin as a standard substance was developed. Two coloration systems including Al(NO3)3-NaNO2-NaOH and AlCl3 were both used. The latter was selected as the optimal determination method by comparing with the results. The main interference of the matrix was discussed. The result showed that the AlCl3 coloration method is simple, accurate and reproducible with good linear relationship, and it can be looked as a reference method for the determination of total flavonoids in herbal materials or natural products.
Key words：Olea europaea L. leaves;Total flavonoids;UV-Vis spectrophotometry;AlCl3 coloration
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