1. Institute of Medicinal Plant, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193，China 2. Bruker Instruments Ltd.，Beijing 100081，China 3. College of Science，China Agricultural University，Beijing 100193，China
Abstract：The objective of the present study was to develop a method for rapid determination of baicalin and total flavonoids in radix scutellariae by near infrared diffuse reflectance spectroscopy. Sixty one samples of radix scutellariae from different areas containing baicalin of 12.24%-21.34% and total flavonoids of 16.08%-26.52% were used. The range of 8 000-4 000 cm-1 of near infrared spectra (NIRS) was selected. Calibration models were established using the PLS(partial least squares). Different spectra pretreatment methods were compared and the optimal model was selected. The study showed that first derivative pretreatments and minimum-maximum normalization methods can be used to extracted spectra information thoroughly to analyze the contents of baicalin and total flavonoids, respectively.The correlation coefficient (r)of baicalin was 0.902 4，SEC was 1.01(standard deviation of the calibration sets) and SEP was 0.876 4(standarddeviation ofthe prediction sets). The correlation coefficient(r) of total flavonoids was 0.952 7，SEC was 0.785 0 and SEP was 0.521 1. Results indicated that near infrared diffuse reflectance spectroscopy method can be used to analyze the main active components in radix scutellariae rapidly.
[1] CUI Lan, YUAN Jing(崔 岚，袁 静). Chinese Journal of Hospital Pharmacy(中国医院药学杂志)，2000，20(11)：685. [2] ZHANG Xi-ping, TIAN Hua, CHENG Qi-hui(张喜平, 田 华, 程琪辉). Chinese Pharmacological Bulletin(中国药理学通报)，2003，19(11)：1212. [3] FU Hong, XIAO Xin-yue, ZHANG Nan-ping, et al(符 洪, 肖新月, 张南平, 等). Chinese Journal of Pharmaceutical Analysis(药物分析杂志)，2003, 23(3)：33. [4] MA Ai-tuan, ZHONG Xiu-hui, MENG Li-gen, et al(马爱团，钟秀会，孟立根，等). Chinese Journal of Veterinary Medicine(国兽医杂志)，2006，42(9)：39. [5] The Pharmacopoeia Commission of the People’s Republic of China(国家药典委员会编). Chinese Pharmacopoeia of the People’s Republic of China(中华人民共和国药典，2005). Beijing：Chemical Industry Press(北京：化学工业出版社)，2005. [6] LIANG Ying, WANG Zhi, HAN Lu-jia(梁 英，王 志，韩鲁佳). Journal of Heilongjiang August First Land Reclamation University(黑龙江八一农垦大学学报)，2006，18(5)：73. [7] LIU Mei-lan, YANG Li-xin, WAN Yuan-hao, et al(刘美兰，杨立新，万元浩，等). Chinese Journal of Pharmaceutical Analysis(药物分析杂志)，2002，22(2)：99. [8] LI Yun-peng, HUANG Yun, WEI Chun-e, et al(李云鹏，黄 芸，魏春娥，等). Journal of Heibei TCM and Pharmacy(河北中医药学报)，2007，22(11)：36. [9] LU Wan-zhen, YUAN Hong-fu, XU Guang-tong, et al(陆婉珍，袁洪福，徐广通，等). Modern Analysis Technique of NIR(现代近红外光谱分析技术). Beijing：China Petrochemical Press(北京: 中国石化出版社), 2000. 170.
