Abstract:A spectrophotometric method for the determination of flavonoids content in Waste Residue of Stevia Rebaudiana was established by comparing the UV-Vis spectra with three spectrophotometric methods: Direct, AlCl3 and NaNO2-Al(NO3)3-NaOH method. The effects of spectrophotometric reaction were studied by analyzing the wavelength, the dosage of color development reagent, reaction time, temperature and pH. The further research was studied by analyzing linearity between the absorbance and the concentration, the stability of this color system, the recovery rate and so on. The results showed that NaNO2-Al(NO3)3-NaOH method was best. The wavelength was 500 nm. The standard sample was rutin. There was a good linear relationship in the ranges of 2~24 μg·mL-1, correlation coefficient R2=0.999 5. The recovery of the additional sample was 96.0%~104.3%. The relative standard deviation(RSD) is respectively 3.75%. The dosages of NaNO2 and Al(NO3)3 both were 1.0 mL, both of their reaction time was 6 min. The dosage of NaOH was 15.0 mL and the reaction time was 15 min. The temperature in the whole process of the reaction must be maintained at 40 ℃ and pH is 5. The spectrophotometric condition was further improved on the determination of flavonoids content. This method, which is simple, rapid and accuracy, can be used in practical application.
Key words:Waste residue of stevia rebaudiana;Total flavonoids;Spectrophotometry;Spectrophotometric conditions
罗序燕,方浩斌*,黄瑞宇,赵东方,祝 婷,郭养养,曾益平,刘仁月,朱 东 . 分光光度法测定甜叶菊废渣中总黄酮的研究 [J]. 光谱学与光谱分析, 2015, 35(10): 2820-2824.
LUO Xu-yan, FANG Hao-bin*, HUANG Rui-yu, ZHAO Dong-fang, ZHU Ting, GUO Yang-yang, ZENG Yi-ping, LIU Ren-yue, ZHU Dong. Research on the Spectrophotometry for Determination of Flavonoids in Waste Residue of Stevia Rebaudiana . SPECTROSCOPY AND SPECTRAL ANALYSIS, 2015, 35(10): 2820-2824.
[1] WEN Kai-xin, WANG Cheng-zhang, YAN Xue-bin, et al(文开新, 王成章, 严学斌, 等). Pratacultural Science(草业科学), 2010, 27(6): 115. [2] ZHU Wen-zhen, MA Long, LI Guo-rong(朱文振, 马 龙, 李国荣). Chinese Bulletin of Life Science(生命科学), 2012, 24(5): 444. [3] Tim T P Cushnie, Andrew J Lamb. International Journal of Antimicrobial Agents, 2005, 26: 343. [4] ZHANG Qiu-yan, ZHANG Fu-ping, CHEN Qiu-hua, et al(张秋燕, 张福平, 陈秋华, 等). Journal of Liaoning University of Traditional Chinese Medicine(辽宁中医药大学学报), 2014, 16(3): 30. [5] Lin Y, Vermeer M A, Bos W, et al. Journal of Agricultural and Food Chemistry, 2011, 59(9): 4496. [6] WANG Ke, WANG Yong, ZHAO Dong-bao, et al(王 柯, 王 勇, 赵东保, 等). Chinese Journal of Analysis Laboratory(分析试验室), 2011, 30(2): 28. [7] MU Hong-lei, GAO Hai-yan, CHEN Hang-jun, et al(穆宏磊, 郜海燕, 陈杭君, 等). Journal of Chinese Institute of Food Science and Technology(中国食品学报), 2010, 10(2): 201. [8] RAN Yue, JIAO Bi-ning, ZHAO Qi-yang, et al(冉 玥, 焦必宁, 赵其阳, 等). Food Science(食品科学). 2013, 34(4): 168. [9] MENG Mei-jia, ZENG Hai-song, LI Jing, et al(孟美佳, 曾海松, 李 静, 等). Chinese Journal of Pharmaceutical Analysis(药物分析杂志), 2010, 30(3): 405. [10] CHI Yu-mei, JU Ling, DENG Hai-shan, et al(池玉梅, 居 羚, 邓海山, 等). Chinese Journal of Analytical Chemistry(分析化学), 2010, 38(6): 893. [11] YAO Xin-sheng(姚新生). Medicinal Chemistry of Natural Products(天然药物化学). Beijing: People’s Medical Publishing House(北京: 人民卫生出版社), 2001. 207. [12] SHI Gao-feng, WANG Hong-yu, WANG Guo-ying, et al(史高峰, 王红玉, 王国英, 等). Chinese Journal of Experimental Traditional Medical Formulae(中国实验方剂学杂志), 2012, 18(22): 62. [13] ZHANG Pei-cheng(张培成). Flavonoids Chemical(黄酮化学). Beijing: Chemical Industry Press(北京: 化学工业出版社), 2008. 239.