Determination of Physiological Indices in Early Kernel at Different Ear Position in Maize(Zea Mays L.) with UV-Visible Spectrophotometry
SHEN Li-xia1,2, WANG Pu2*, SUN Xi-huan1
1. College of Water Conservancy and Engineering, Taiyuan University of Technology, Taiyuan 030024, China 2. College of Agronomy and Biotechnology, China Agricultural University, Beijing 100094, China
Abstract：To examine the difference between early kernels at different ear position in maize and the effect of nitrogen supply rates on kernel development, physiological indices in apical and mid-basal kernel 5-20 days after pollination in maize under different nitrogen supply rates (0, 120, 180 and 240 kg·ha-1) were determined with UV-Vis spectrophotometry. The results showed that nitrogen supply obviously increased the contents of soluble sugar, sucrose and starch, and the activities of enzymes related to sucrose inversion and starch synthesis. Twenty days after pollination, the kernel volume, dry weight and the contents of soluble sugar, sucrose and starch in apical kernel under nitrogen supply rate of 180 kg·ha-1 were higher than those with other treatments. Five to twenty days after pollination, higher activities of acid sucrose invertase (AI), neutral sucrose invertase (NI), sucrose synthase (SS), ADPGase and starch synthase in apical kernel were also obtained under nitrogen supply rate of 180 kg·ha-1, indicating that the sucrose utilization and starch synthesis were improved, the kernel development was promoted and the kernel abortion was reduced, which resulted in higher yield.
申丽霞1，2, 王 璞2*, 孙西欢1 . 紫外-可见分光光度法测定玉米不同部位籽粒早期发育的生理指标[J]. 光谱学与光谱分析, 2009, 29(09): 2551-2554.
SHEN Li-xia1,2, WANG Pu2*, SUN Xi-huan1. Determination of Physiological Indices in Early Kernel at Different Ear Position in Maize(Zea Mays L.) with UV-Visible Spectrophotometry . SPECTROSCOPY AND SPECTRAL ANALYSIS, 2009, 29(09): 2551-2554.
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