Abstract：Cell cycle is the most important process in life, and embodies all the physiological processes in the cell. In order to reflect the process of cell growth simply and accurately, the authors took cell cycle into account when the Hela cell spectral model was designed. The artificially induced cell synchronization method was employed to make Hela cell in G1, S, G2 and M phases of the cell cycle. The UV absorption spectra of these Hela cell samples were measured. The absorbability of aromatic amino acid, protein and nucleic acid in different stages of the cell cycle showed the changes in cell cycle. The subsection linear regression model of cell UV absorption spectrum was designed according to the relationship between the stage and the absorbance of samples in different phase at 204 and 260 nm. The models can be used to estimate the cell cycle after experimental verification and provide a new method for analyzing cell cycle and building cell model.
Key words：UV absorption spectrum;Cervical cancer cell;Cell spectral model;Cell cycle
林晓钢,潘英俊,郭永彩,张 玮,赵一石. 基于紫外吸收光谱的癌细胞模型研究[J]. 光谱学与光谱分析, 2009, 29(09): 2547-2550.
LIN Xiao-gang, PAN Ying-jun, GUO Yong-cai, ZHANG Wei, ZHAO Yi-shi . The Study of Cervical Cancer Cells Model Based on UV Absorption Spectrum. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2009, 29(09): 2547-2550.
