Quantitative Measurement of Fe(Ⅱ) in Human Blood with Spectral Analysis Method
XU Xiao-feng1, ZHAO Fu-li1*, WANG Shuang1,YE Bao-hui2
1. School of Physics Science and Engineering Technology, Sun Yat-sen University, Guangzhou 510275, China 2. School of Chemistry and Chemical Engineering, Sun Yat-sen University, Guangzhou 510275, China
Abstract：Since the absorption spectra of Fe(Ⅱ) ions solutions vary with the concentration of Fe(Ⅱ) irons, the authors tried to establish a direct measurement of active haemachrome in human blood through spectral analysis, and successfully made an experiment on it. The authors prepared Fe(Ⅱ) ions solutions with various concentrations chemically, using 1,10-phenanthroline (phen) as chromogenic agent. After the absorption spectra of these solutions were analyzed, Gaussion fitting at absorption peaks was performed to them. The linear fitting figure showed that the areas of Gaussion fitting curves correspond to the concentrations of Fe(Ⅱ) irons. Thus, the concentration of Fe(Ⅱ) in human blood was figured out in this way, and the result fitted quite well the actual concentration. As an optimized method, this creative idea is more accurate and precise. It will probably contribute to the development of new devices for measuring Fe(Ⅱ) ions in human blood.
Key words：Absorption spectrum;Concentration of Fe(Ⅱ);Human Blood
[1] KONG Xiang-rui(孔祥瑞). The Effect of Trace Element in Nutrition, Physiology and Clinical Application and Technology and Technology Press(必需微量元素的营养、生理及临床意义). Hefei: Anhui Science and Technology Press(合肥：安徽科技出版社), 1982. 194. [2] JIAO Zhi-ping(焦治平). Anhui Medical and Pharmaceutical Journal(安徽医药)，2002, 6(1): 42. [3] HE Bang-ping, WU Qiu-ye, PAN Ya-ju, et al(何邦平，吴秋业，潘亚菊，等). Journal of Chinese Clinical Medicine(中华临床医药)，2004, 5(7): 11. [4] Ministry of Health in People’s Republic of China(中华人民共和国卫生部医政司编). National Regulation for Clinic(Second Edition)(全国临床检验操作规程，第2版). Nanjing: Southeast University Press(南京：东南大学出版社)，1997. 200. [5] CAO Jian-ming, SHEN Cai-cheng, LI Min(曹建明，沈财成，李 敏). China Public Health(中国公共卫生)，2002, 18(11): 1384. [6] CAO Jian-ming, XIAO Hong-wu, JIANG Hong-ying, et al(曹建明，肖洪武，姜红鹰，等). Chinese Journal of Medical Laboratory Technology(中华检验医学杂志)，2004, 27(3): 137. [7] DONG Shun-fu, ZHU Zhi-guo, LIU Wei-ming, et al(董顺福，朱志国，刘伟明，等). Spectroscopy and Spectral Analysis (光谱学与光谱分析)，2001, 21(3): 391. [8] LI Ren-qiang, CHEN Yao, JIANG Feng-yi(李任强，陈 瑶，江凤仪). Spectroscopy and Spectral Analysis(光谱学与光谱分析)，2004, 24(1): 95. [9] LI Jin-min(李进民). Journal of Datong Vocational College(大同职业技术学院学报)，2004, 18(2): 72. [10] YAO Qun-feng, XU Shun-qing, ZHOU Yi-kai(姚群峰，徐顺清，周宜开). Chinese Journal of Health Laboratory Technology(中国卫生检验杂志)，2000, 10(1): 3. [11] Michael Hollas J. High Resolution Spectroscopy. John Wiley & Sons, 1982.
