Study on BS-12 as a New Probe for the Determination of DNA by Resonance Light Scattering
CHEN Zhan-guang1, 2, LIAO Xu-hong1, HAN Ya-li1, YANG Ying-yi1, LU-Feng1, DING Wei-feng1
1. Science College, Shantou University, Shantou 515063, China 2. College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China
Abstract：A new method has been developed for the determination of DNA by resonance light scattering with dodecyl dimethyl betaine (BS-12) in aqueous solution as a new probe. At pH 9.3, the interactions of BS-12 and DNA gave strong RLS signals at 388.0 nm. Linear relationships were found between the enhanced intensity of RLS and the concentration of DNAs in the range 0.25-12.0 μg·mL-1for fsDNA and 0.25-11.0 μg·mL-1 for ctDNA. The limits of detection were 0.15 ng·mL-1 and 0.16 ng·mL-1 for fsDNA and ctDNA, respectively. The method was applied to the determination of synthetic samples with satisfactory results.
陈展光1，2，廖栩泓1，韩雅莉1，杨英宜1，卢 峰1，丁卫锋1 . 两性离子表面活性剂BS-12与DNA作用的共振光散射光谱[J]. 光谱学与光谱分析, 2005, 25(10): 1680-1683.
CHEN Zhan-guang1, 2, LIAO Xu-hong1, HAN Ya-li1, YANG Ying-yi1, LU-Feng1, DING Wei-feng1 . Study on BS-12 as a New Probe for the Determination of DNA by Resonance Light Scattering. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2005, 25(10): 1680-1683.
[1] HUANG Cheng-zhi, LI Ke-an, TONG Shen-yang. Anal. Chim. Acta, 1997, 345: 235. [2] ZHU Qing-zhi，YANG Huang-hao, XU Jin-gou, et al. Anal. Chim. Acta, 1999, 394(2-3): 177. [3] Pasternack P F, Collings P J, Giannetto A, et al. J. Am. Chem. Soc., 1993, 115: 5393. [4] Pasternack P F, Collings P J. Science, 1995, 269: 935. [5] LIU Shao-pu(刘绍璞). Advances in Analytical Chemistry(分析化学新进展)，WANG Er-kang(汪尔康主编). Beijing: Science Press(北京：科学出版社)，2002. 280. [6] CHEN Zhan-guang, LIAO Xu-hong, LI Dan, et al. Chem. J. on Internet, 2003, 5(10): 82. [7] LIAO Xu-hong, CHEN Zhan-guang, YANG Ying-yi, et al(廖栩泓，陈展光，杨英宜, 等). J. of Instrumental Analysis(分析测试学报), 2004, 23(3): 118. [8] LIU Shao-pu, HU Xiao-li, LUO Hong-qun, et al(刘绍璞，胡小莉，罗红群, 等). Science in China Series B(中国科学B辑), 2002, 32(1): 18. [9] FANG Yun, XIA Yong-mei(方 云，夏咏梅). China Daily Chemical Industry(日用化学工业), 2000, 30(3): 53. [10] LI Fang, LI Gan-zuo, WANG Han-qing, et al(李 芳, 李干佐, 汪汉卿, 等). Chem. J. of Chinese Universities(高等学校化学学报), 1998, 19(7): 1117. [11] FANG Yun, XIA Yong-mei(方 云，夏咏梅). China Daily Chemical Industry (日用化学工业), 2000, 30(5): 55. [12] SU Jie-shu, CHEN Xiao-ming, LONG Yun-fei, et al(苏界殊，陈小明，龙云飞，等). Spectroscopy and Spectral Analysis(光谱学与光谱分析)，2004，24(1)：37.
