The Effects of Antioxidant, DPPH and Human Serum Albumin Ternary System Studied by Spectrophotometry
TANG Xiao-yu1, LUO Yun-jing1*, LI Shu-guang2, LIN Tai-feng1, WANG Yan1
1. Beijing Key Laboratory of Environmental and Viral Oncology, College of Life Science and Bioengieering, Beijing University of Technology, Beijing 100124, China
2. Food Center, Beijing Industrial Technician College, Beijing 100023, China
Abstract：In this study, the natural antioxidants myricetin, morin, capsaicin and betaine were selected as the research objects, and the interaction between several antioxidants, DPPH free radical and human albumin was studied by synchronous fluorescence spectroscopy and three-dimensional fluorescence spectroscopy. Results showed that capsaicin, betaine, VC do not have quench effects with human serum albumin, and myricetin, morin and DPPH have quench effects with human albumin. The reactions are static quenching caused by stable complexes, combining hydrophobic interaction with HSA.,The binding sites are 1, the main binding sites are near the tryptophan groups, and DPPH and human serum albumin’s quenching process changes the structure of human serum protein hydrophobicity, causes protein conformation changes, and the interaction between myricetin, morin and human albumin does notcause its conformation changes. Fluorescence spectroscopy were used to study the inhibitory effect of several antioxidants on DPPH-induced direct damage to human serum albumin. The inhibition rate of myricetin, morin, capsaicin, betaine and VC to DPPH damage HSA was 25%, 18.30%, 85.38%, 4.02% and 84.58%. Capsaicin inhibits the damage of human serum albumin by inhibiting the action of DPPH. The binary system reaction results showed that myricetin reacted with Morin ternary system to form competitive binding sites with DPPH, and Myricetin and Morin inhibit DPPH damage to human serum albumin by occupying binding sites, while Betaine can neither occupy the binding sites nor scavenge free radicals, so the inhibition ability is the weakest. The results showed that the inhibitory ability of several natural antioxidants is closely related to the main functional groups in the molecular structure.
汤小玉，罗云敬，李曙光，林太凤，王 妍. 光谱法研究抗氧化剂、DPPH与人血清蛋白三元体系的作用[J]. 光谱学与光谱分析, 2019, 39(10): 3122-2128.
TANG Xiao-yu, LUO Yun-jing, LI Shu-guang, LIN Tai-feng, WANG Yan. The Effects of Antioxidant, DPPH and Human Serum Albumin Ternary System Studied by Spectrophotometry. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2019, 39(10): 3122-2128.
[1] WEI Xian-ya, YIN Li-qin, ZHONG Cheng, et al(韦献雅, 殷丽琴, 钟 成, 等). Food Science(食品科学), 2014, 35(9): 317.
[2] Nurdianah H F, Ahmad Firdaus A H, EshaifolAzam O, et al. International Food Research Journal, 2016, 23(1): 403.
[3] LI Cai-yi, ZHANG Guo-ying, DONG Xiu-ying, et al(李采怿, 张国英, 董秀英, 等). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2017, 37(8): 2402.
[4] LI Chang-qin, YAO Chen, ZHU Rong-yao, et al(李昌勤, 姚 辰, 朱荣遥, 等). China Journal of Chinese Materia Medica(中国中药杂志), 2016, 41(9): 1670.
[5] Musa K H, Abdullah A, Alhaiqi A. Food Chemistry, 2016, 194(4): 705.
[6] Oliveira G K F, Tormin T F, Sousa R M F, et al. Food Chemistry, 2016, 192: 691.
[7] Hazra M, Dolai T, Pandey A, et al. Journal of Saudi Chemical Society, 2017, 21: S240.
[8] Dangkoobf F, Housaindokht M R, Asoodeh A, et al. Spectrochimica Acta Part A, 2014, 137: 1106.
[9] Zhang C, Zhang G, Liao Y, et al. Food Chemistry, 2017, 221: 1569.
[10] Jabeen E, Janjua N K, Ahmed S, et al. Electrochimica Acta, 2017, 258.
[11] LIU An, WANG Zhen, LIU Lin-feng, et al(刘 安, 王 振, 刘林峰, 等). Food & Machinery(食品与机械), 2015，(6): 179.
[12] WANG Ce, LI Xia, DENG Shao-ying, et al(王 策, 李 侠, 邓少颖, 等). Scientia Agricultura Sinica(中国农业科学), 2017, 50(15): 3013.
[13] LI Xue-peng, ZHOU Kai, WANG Jin-xiang, et al(李学鹏, 周 凯, 王金厢, 等). Journal of Chinese Institute of Food Science and Technology(中国食品学报), 2014, 14(6): 19.
[14] AN Peng-jiao, YU Nan-nan, SUN Rui-sheng, et al(安鹏姣, 于楠楠, 孙睿声, 等). Chemical Journal of Chinese Universities(高等学校化学学报), 2017, 38(8): 1354.
