|
|
|
|
|
|
Determination of Carotenoids Contents in Tea Leaves Based on Raman Spectroscopy |
LI Xiao-li, XU Kai-wen, HE Yong* |
College of Biosystems Engineering and Food Science,Zhejiang University,Hangzhou 310058, China |
|
|
Abstract Carotenoids are one of the most important components to reflect the physiological state of plant, such as environmental stress,photosynthesis as well as growing state. An effective and nondestructive detection method for the contents of carotenoids in Longjing-43 leaves was established based on Raman spectroscopy in this research. A total of 315 tea sample were used for the spectral collection and spectrophotometry determination. Quantitative models were established to predict the content of carotenoids. Firstly, in order to eliminate the interference from noise, 5 preprocessing methods were applied before calibration stage. Partial least square regression (PLS) was applied as calibration method to establish the Raman spectra quantitative model of the carotenoids in Longjing-43’s leaves. The results of PLS models based on these methods were used to evaluate the performances of these pretreatments. The best performance was achieved with wavelet transformed spectra, obtaining correlation coefficient (r) value of 0.817 and 0.786 for validation and prediction, respectively. Secondly, to further explore the Raman spectral response properties of carotenoids in tea, Successive Projections Algorithm (SPA) was applied to extract the characteristic wavenumbers for carotenoids in tea. Then, the selected 17 wavenumbers were used to build PLS model and good results are reached, obtaining Rv of 0.808 and Rp of 0.777. Based on the PLS model, this article studied the carotenoids content of tea leaves in 4 different growth periods. Results indicated that the content of carotenoids was firstly increased then decreased. The above results revealed that it was feasible to apply Raman spectroscopy for the determination of the carotenoids content in Longjing-43 leaves.
|
Received: 2016-07-27
Accepted: 2016-12-05
|
|
Corresponding Authors:
HE Yong
E-mail: yhe@zju.edu.cn
|
|
[1] Li Xiaoli, Nie Pengcheng, Qiu Zhengjun, et. al. Expert Systems with Applications, 2011, 38(9): 11149.
[2] CHEN Hong-de(陈洪德). Journal of Anhui Agri. Sci.(安徽农业科学), 2013,(20): 8529.
[3] Cen H, Bao Y, Yong H, et al. Journal of Food Engineering, 2007, 82(2): 253.
[4] HUO Ming-ming(霍明明). Thesis of Doctor Degree of Harbin Institue of Technology(哈尔滨工业大学), 2009.
[5] ZHAO Xing, LI Dong-fei, CHEN Yuan-zheng(赵 星,李东飞,陈元正). Chinese Journal of Light Scattering(光散射学报),2012, 24(3): 289.
[6] Bhosale P, Ermakov I V, Ermakova M R, et al. Journal of Agricultural and Food Chemistry, 2004, 52(11): 3281.
[7] Schulz H, Baranska M, Baranski R. Biopolymers, 2005, 77(4): 212.
[8] Baranska M, Schutze W, Schulz H. Analytical Chemistry, 2006, 78(24): 8456.
[9] Bicanic D, Dimitrovski D, Luterotti S, et al. Food Biophysics, 2010, 5(1): 24.
[10] Zhao Yanru, Li Xiaoli, Yu Keqiang, et al. Scientific Reports, 2016, 6.
[11] Gonzálvez A G, Martínez N L, Telle H H, et al. Chemical Physics Letters, 2013, 559: 26.
[12] Rivera S M, Canelagarayoa R. Journal of Chromatography A, 2012, 1224(14): 1.
[13] Greene P R, Bain C D. Colloids & Surfaces B Biointerfaces, 2005, 45(3-4): 174.
[14] ZHU Zi-ying, GU Ren-ao, LU Tian-hong(朱自莹,顾仁敖,陆天虹). Raman Spectroscopy Application in Chemistry(拉曼光谱在化学中的应用). Shenyang: Northeastern Universtiy Press(沈阳:东北大学出版社), 1998.
[15] Sulyok M, Haberhauer-Troyer C, Rosenberg E. Physical Chemistry Chemical Physics, 2009, 11(48): 11471.
[16] WANG Feng, CHEN Yu-zhen, WANG Xiu-ping, et al(王 峰,陈玉真,王秀萍,等). Journal of Tea Science(茶叶科学), 2016,(1): 77.
[17] Malgorzata Baranskaa, Rafal Baranskib, Ewa Grzebelusb, et al. Vibrational Spectroscopy, 2011, 56(2): 166. |
[1] |
LIU Lu-yao1, ZHANG Bing-jian1,2*, YANG Hong3, ZHANG Qiong3. The Analysis of the Colored Paintings from the Yanxi Hall in the Forbidden City[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(07): 2054-2063. |
[2] |
ZHU Ya-ming1, 2, ZHAO Xue-fei1, 2*, GAO Li-juan1, CHENG Jun-xia1. Quantitative Analysis of Structure Changes on Refined Coal Tar Pitch with Curve-Fitted of FTIR Spectrum in Thermal Conversion Process[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(07): 2076-2080. |
[3] |
CHEN Sheng, ZHANG Xun, XU Feng*. Study on Cell Wall Deconstruction of Pinus Massoniana during Dilute Acid Pretreatment with Confocal Raman Microscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(07): 2136-2142. |
[4] |
HE Qing1, JIANG Qin1, XING Li-da2, 3, AN Yan-fei1, HOU Jie4, HU Yi5. Microstructure and Raman Spectra Characteristics of Dinosaur Eggs from Qiyunshan, Anhui Province[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(07): 2143-2148. |
[5] |
TAN Ai-ling1, WANG Si-yuan1, ZHAO Yong2, ZHOU Kun-peng1, LU Zhang-jian1. Research on Vinegar Brand Traceability Based on Three-Dimensional Fluorescence Spectra and Quaternion Principal Component Analysis[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(07): 2163-2169. |
[6] |
KE Ke1, 2, Lü Yong1, 2, YI Can-can1, 2, 3*. Improvement of Convex Optimization Baseline Correction in Laser-Induced Breakdown Spectral Quantitative Analysis[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(07): 2256-2261. |
[7] |
CAI Zong-qi1, FENG Wei-wei1, 2*, WANG Chuan-yuan1. The Study of Oil Film Thickness Measurement on Water Surface Based on Laser Raman Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(06): 1661-1664. |
[8] |
WU Jun, YOU Jing-lin*, WANG Yuan-yuan, WANG Jian, WANG Min, Lü Xiu-mei. Raman Spectroscopic Study of Li2B4O7 Crystal and Melt Structure[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(06): 1736-1740. |
[9] |
ZHANG Lu-tao, ZHOU Guang-ming*, ZHANG Cai-hong, LUO Dan. The Preparation of the New Membrane-Like Gold Nanoparticles Substrate and the Study of Its Raman Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(06): 1741-1746. |
[10] |
CHEN Si-yuan1, YANG Miao1, LIU Xiao-yun2*, ZHA Liu-sheng1*. Study on Au@Ag Core-Shell Composite Bimetallic Nanorods Laoding Filter Paper as SERS Substrate[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(06): 1747-1752. |
[11] |
MA Ying1, WANG Qi2, QIU Zhi-li1*, LU Tai-jin3, LI Liu-fen1, CHEN Hua3, DENG Xiao-qin1, BO Hao-nan1. In-Situ Raman Spectroscopy Testing and Genesis of Graphite Inclusions in Alluvial Diamonds from Hunan[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(06): 1753-1757. |
[12] |
LIU Jia1, YANG Ming-xing1, 2*, DI Jing-ru1, 2, HE Chong2. Spectra Characterization of the Uvarovite in Anorthitic Jade[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(06): 1758-1762. |
[13] |
OUYANG Ai-guo, ZHANG Yu, TANG Tian-yi, LIU Yan-de. Study on Density, Viscosity and Ethanol Content of Ethanol Diesel Based on Raman Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(06): 1772-1778. |
[14] |
PENG Heng, LIU Shuai, CHEN Xiang-bai*. Raman Study of Perovskite (C6H5CH2NH3)2PbBr4[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(06): 1763-1765. |
[15] |
ZHONG Qian1, 2, 3, WU Qiong2, 3, LIAO Zong-ting1, 2, 3*, ZHOU Zheng-yu1, 2, 3. Vibrational Spectral Characteristics of Ensignia Actinolite Jade from Guangxi, China[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(06): 1786-1792. |
|
|
|
|