光谱学与光谱分析 |
|
|
|
|
|
Study on the Conformation of Soybean Selenoprotein Solution with Spectroscopy Methods |
HU Yong, WU Xiao-yong*, ZHONG Nan-jing, XU Jin-rui |
School of Food Science, Guangdong Pharmaceutical University, Zhongshan 528458, China |
|
|
Abstract The structure characteristic of soybean selenoprotein and soy protein isolate (SPI) were investigated with fluorescence, ultraviolet and Fourier transform infrared (FTIR) spectrum. The unfolding process of two proteins was analyzed with fluorescence phase diagram method. The stability of emulsion properties and the influence of concentration, temperature and pH on the conformation of soy selenoproteins were also determined. The results indicated that the covalent disulfide bond of soybean selenoprotein molecules was damaged; the hydrogen bonding become weak; the hydrophobic interactions were enhanced and the protein chain molecules were extended. Soybean selenoprotein displayed only “folding” and “loose” state in solution, which illustrated soybean selenoprotein more tend to hydrolysis when compared with soybean protein. With temperature increasing, the fluorescence quenching effect occurred and the hydrophobicity of soy selenoproteins was also gradually increased, which reflected the protein molecules tends to be folded. In the range of pH 2.8~8.0, the Trp residue of soybean selenoprotein was mainly distributed in the polarity of the external environment and presented different conformational change on both sides of the isoelectric point under different pH value. In acidic environment, the soybean selenoprotein was easy to appear conformational transition from loose to fold. But it was conducive for soybean selenoprotein to existence in loose structure in alkaline conditions. In addition, the emulsifying properties of soybean selenoprotein were analyzed based on UV spectral data. Results showed that lower temperature helps to enhancement the emulsification but unfavorable the stability of the soybean selenoprotein.
|
Received: 2015-05-13
Accepted: 2015-09-18
|
|
Corresponding Authors:
WU Xiao-yong
E-mail: perryfe@163.com
|
|
[1] TIAN Jun-mei, ZHANG Ding, FU Rui-juan, et al(田俊梅, 张 丁, 付瑞娟, 等). Soybean Science(大豆科学), 2010, 29(3): 534. [2] Thavarajah D, Ruszkowski J, Vandenberg A. Journal of Agricultural and Food Chemistry, 2008, 56: 10747. [3] Finley J W. Journal of the Science of Food and Agriculture, 2007, 87(10): 1620. [4] LIU Hong, YANG Hui, AI Min-xian(刘 红, 阳 辉, 艾民仙). Acta Nutrimenta Sinica(营养学报), 2012, 34(3): 238. [5] Eckenro B, Harris K, Turanov A, et al. Biochemistry, 2006, 45:5158. [6] HUANG Yan-sheng, NING Zheng-xiang, WU Xiao-yong(黄延盛, 宁正祥, 吴小勇). Modern Food Science and Technology(现代食品科技), 2013, 29(6): 1273. [7] LIU Ying, PENG Chang-de, LAN Xiu-feng, et al(刘 莹、彭长德、兰秀风,等). Acta Physica Sinica(物理学报), 2005, 54(11): 5455. [8] Philip G R, Brian R G, DavidA F G, et al. Journal of Agricultural and Food Chemistry, 2007, 55: 516. [9] ZHANG Tan, BIAN Liu-jiao(张 潭, 边六交). Chemical Journal of Chinese Universities(高等学校化学学报),2011, 32(7): 1497. [10] LONG Guo-hui, JI Yuan, PAN Hong-bin, et al(龙国徽, 纪 媛, 潘洪斌, 等). Journal of Jilin University(吉林大学学报), 2014, 52(4): 840. [11] PENG Gang, LIU Bai-ling, ZHAO Chun-xia, et al(彭 刚, 刘白玲, 赵春霞, 等). Journal of the Graduate School of the Chinese Academy of Sciences(中国科学院研究生院学报), 2011, 28(1): 12. [12] WANG Zhong-jiang, JIANG Lian-zhou, WEI Dong-xu, et al.(王中江, 江连州, 魏冬旭, 等). Food Science(食品科学),2012, 33(11): 47. |
[1] |
WANG Fei1,2, LI Huan1, YANG Ke1, TEULET Philippe2, CRESSAULT Yann2. Computation of Equilibrium Compositions of GMAW Arc Plasmas and Its Applications in Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(07): 1998-2003. |
[2] |
XU Chen1, 2, HUA Xue-ming1, 2*, YE Ding-jian1, 2, MA Xiao-li1, 2, LI Fang1, 2, HUANG Ye1, 2. Study of the Effect of Interference during Multi-Wire GMAW Based on Spectral Diagnosis Technique[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(07): 1993-1997. |
[3] |
NIE Mei-tong1,2, XU De-gang1,2*, WANG Yu-ye1,2*, TANG Long-huang1,2, HE Yi-xin1,2, LIU Hong-xiang1,2, YAO Jian-quan1,2. Investigation on Characteristics of Edible Oil Spectra with Terahertz Time-Domain Attenuated Total Reflection Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(07): 2016-2020. |
[4] |
LI Zheng-hui1,3, YAO Shun-chun1,3*, LU Wei-ye2, ZHU Xiao-rui1,3, ZOU Li-chang1,3, LI Yue-sheng2, LU Zhi-min1,3. Study on Temperature Correction Method of CO2 Measurement by TDLAS[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(07): 2048-2053. |
[5] |
HU Wen-bin1, MA Zhi-min1*, TIAN Meng1, ZHAO Xiao-hong1, HU Xiang-yang2. Multispectral-Based Particle Image Velocimetry[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(07): 2038-2043. |
[6] |
LIU Lu-yao1, ZHANG Bing-jian1,2*, YANG Hong3, ZHANG Qiong3. The Analysis of the Colored Paintings from the Yanxi Hall in the Forbidden City[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(07): 2054-2063. |
[7] |
LI Xiao-jun, HE Xian-li, SONG Rui-juan. Theoretical Study of Structures, Stabilities, and Infrared Spectra of the Alkali-Metal (Li2F)nM (M=Li, Na, K; n=1, 2) Clusters[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(07): 2064-2069. |
[8] |
JIAN Kuo1,2,LIU Shun-xi4,CHEN Yi-lin3,FU Xue-hai2,3*. Infrared Spectroscopic Study on the Structure Evolution of Low Rank Coal and Its Correlation with Carbon Isotope of Alkane Gas in Pyrolysis Process[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(07): 2070-2075. |
[9] |
WANG Shuai1, XU Jun-ping1, WANG Nan1, LEI Wan-ying1, FAN Xi-yan1, DOU Sen2, 3*. Structural Characteristics of Mineral-Microbial Residues Formed by Microbial Utilization of Lignin Joined with Fe, Al, Mn-Oxides Based on FT-IR and SEM Techniques[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(07): 2086-2093. |
[10] |
WANG Wen-xiu, PENG Yan-kun*, FANG Xiao-qian, BU Xiao-pu. Characteristic Variables Optimization for TVB-N in Pork Based on Two-Dimensional Correlation Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(07): 2094-2100. |
[11] |
HU Hua-ling1, 2, 3, LI Meng2, 3*, HE Xiao-song2, 3, XI Bei-dou2, 3, ZHANG Hui2, 3, LI Dan2, 3, HUANG Cai-hong2, 3, TAN Wen-bing2, 3. FTIR Spectral Characteristics of Rice Plant Growing in Mercury Contaminated Soil[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(07): 2081-2085. |
[12] |
LE Ba Tuan1, 3, XIAO Dong1*, MAO Ya-chun2, SONG Liang2, HE Da-kuo1, LIU Shan-jun2. Coal Classification Based on Visible, Near-Infrared Spectroscopy and CNN-ELM Algorithm[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(07): 2107-2112. |
[13] |
WANG Dong, LIU Shan-jun*, MAO Ya-chun, WANG Yue, LI Tian-zi. A Method Based on Thermal Infrared Spectrum for Analysis of SiO2 Content in Anshan-Type Iron[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(07): 2101-2106. |
[14] |
MA Dian-xu1, LIU Gang1*, OU Quan-hong1, YU Hai-chao1, LI Hui-mei1, SHI You-ming2. Discrimination of Common Wild Mushrooms by FTIR and Two-Dimensional Correlation Infrared Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(07): 2113-2122. |
[15] |
DAI Li-li, SHI Guang-hai*, YUAN Ye, WANG Mei-li, WANG Yan. Infrared Spectroscopic Characteristics of Borneo and Madagascar Copal Resins and Rapid Identification between Them and Ambers with Similar Appearances[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(07): 2123-2131. |
|
|
|
|