| 光谱学与光谱分析 |
|
|
|
|
|
| The Investigation on the Interaction of Colistin Sulfate with Bovine Serum Albumin with Resonance Light Scattering Spectroscopy |
| ZHANG Qiu-ju, LIU Bao-sheng*, LI Gai-xia, HAN Rong, Lü Yun-kai |
| Key Laboratory of Analytical Science and Technology of Hebei Province, College of Chemistry & Environmental Science, Hebei University, Baoding 071002, China |
|
|
|
|
Abstract The interaction between colistin sulfate (CS) with bovine serum albumin in physiological buffer (pH 7.4) was investigated with resonance light scattering spectroscopy at 298, 310, and 318 K. The analysis of data indicated that quenching mechanism of BSA-CS was probably static. The value of n was approximately 1, indicating there was only a single class of binding sites on BSA for CS compounds. The thermodynamic parameters were calculated at different temperatures, implying that the interaction was spontaneous and electrostatic force played major role in the binding between CS and BSA. The values of nH were equal to 1 at different temperatures, indicating there was non-cooperative reaction between BSA and CS. The feasibility of resonance light scattering spectroscopy was verified by fluorescence quenching spectroscopy. The quenching reactive parameters (KSV，Kq，n，Ka) from two methods were similar, suggesting resonance light scattering spectroscopy could be used to study the binding interaction between protein and drugs. Resonance light scattering spectroscopy can be used to explore the substance without intrinsic fluorescence, suggesting that the application of resonance light scattering spectroscopy broadens the understanding of the interaction between small molecules and protein.
|
|
Received: 2015-06-09
Accepted: 2015-10-25
|
|
|
|
Corresponding Authors:
LIU Bao-sheng
E-mail: lbs@hbu.edu.cn
|
|
[1] Vignesh G, Manojkumar Y, Sugumar K, et al. J. Lumin., 2015, 157: 297.
[2] Guo X J, Li X Z, Jiang Y C, et al. J. Lumin., 2014, 149: 353.
[3] Feng D Q, Liu G L, Wang W. J. Mater. Chem. B, 2015, 3(10): 2083.
[4] Chen Z G, Wang Z, Chen J H, et al. Eur. J. Med. Chem., 2013, 66: 380.
[5] HUANG Xin-hua, SHU Wei-qun, LI Yuan-fang, et al(黄新华，舒为群，李原芳，等). Chinese J. Anal. Chem.(分析化学), 2001, 29(3): 271.
[6] Chen X, Liu G L, Liang S W, et al. Anal. Methods-UK, 2012, 4(6): 1546.
[7] Luo H Q, Liu S P, Li N B, et al. Anal. Chim. Acta, 2002, 468(2): 275.
[8] Yin F B, Wang D L, Li Z F, et al. Bioresource Technol., 2015, 177: 188.
[9] Toprak M, Ark M. Luminescence, 2014, 29(7): 805.
[10] Chen D D, Xie J H, Wu Q, et al. J. Lumin., 2015, 160: 245.
[11] Chen Y, Wang R J, Wang S S, et al. B. Korean Chem. Soc., 2011, 32(9): 3261.
[12] Shu Y, Xue W W, Xu X Y, et al. Food Chem., 2015, 173: 31.
[13] Wang Y Q, Zhang H M, Zhang G C, et al. J. Lumin., 2007, 126(1): 211.
[14] CHONG Bao-hong, LIU Bao-sheng, LI Zhi-yun, et al(种宝红，刘保生，李志云，等). Chin. J. Lumin.(发光学报), 2013, 34(10): 1380.
[15] Yan X N, Liu B S, Chong B H, et al. J. Lumin., 2013, 142: 155.
[16] Yu X Y, Yao Q, Li W, et al. J. Solution Chem., 2012, 41(10): 1747.
[17] Xu H L, Yao N N, Li G Y, et al. Spectrosc. Lett., 2014, 47(2): 119.
[18] Yu X Y, Liao Z X, Jiang B F, et al. Spectrochim. Acta A, 2015, 137: 129.
[19] LIU Bao-sheng, WANG Jing, XUE Chun-li, et al(刘保生，王 晶，薛春丽，等). Chin. J. Lumin.(发光学报), 2011, 32(6): 628.
[20] ZHANG Hong-ying, CHEN Ning-sheng, ZHANG Wen-long, et al(张红颖，陈宁生，张文龙，等). Chemical Research and Application(化学研究与应用), 2014, 26(9): 1386. |
| [1] |
LI Jin-zhi1, LIU Chang-jin1, 4*, SHE Zhi-yu2, ZHOU Biao2, XIE Zhi-yong2, ZHANG Jun-bing3, JIANG Shen-hua2, 4*. Antiglycation Activity on LDL of Clove Essential Oil and the Interaction of Its Most Abundant Component—Eugenol With Bovine Serum Albumin[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(01): 324-332. |
| [2] |
WANG Jun1, WANG Zhou-li2, CHENG Jing-jing1. Interaction Between Tartrazine and Bovine Serum Albumin Using Multispectral Method and Molecular Docking[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(03): 904-909. |
| [3] |
WANG Xiao-xia1,3*, WU Hao1, NIE Zhi-hua2, MA Li-tong1,3*, CUI Jin-long1, SAI Hua-zheng1, CHENG Jian-guo1. Study on the Interaction Between Fulvic Acid and Bovine Serum Albumin by Multispectral and Molecular Docking[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2021, 41(09): 2904-2910. |
| [4] |
ZHANG Chuan-ying1, PENG Xin1*, RAO Heng-jun2, QI Wei2, SU Rong-xin2, HE Zhi-min2. Spectroscopic Studies on the Interaction Between Salvianolic Acid B and Bovine Serum Albumin[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2021, 41(06): 1701-1707. |
| [5] |
TUO Xun1, SONG Ji-min1, FU Hao2, LÜ Xiao-lan1*. Study on Interaction Between Hexabromocyclododecane and Bovine Serum Albumin by Spectroscopy and Computer Simulation Methods[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2021, 41(05): 1487-1492. |
| [6] |
LI Xue1, WANG Li1, LIU Guang-xian1*, TU Zong-cai2. Effect of Urea on Glycosylation of BSA Based on Spectral Techniques[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2021, 41(02): 478-483. |
| [7] |
LI Xing-xing, ZHANG Xiang, HUANG Xue-song*. Interaction Between Three Sulfur-Containing Amino Acids in Garlic and Bovine Serum Albumin Determined by Fluorescence Spectrometry[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2020, 40(11): 3483-3488. |
| [8] |
HUANG Fang1, LIU Ming-xue1, 2*, XIONG Jie1, CHEN Lü-qi1, GAO Zhu-xin1, CHEN Hui-ming1, WANG Dan-ni1. Effect of Far-Infrared Ceramic Powder on the Interaction Between Essential Oil and BSA[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2020, 40(08): 2358-2365. |
| [9] |
WANG Xiao-xia1*, NIE Zhi-hua2, MA Li-tong1, CUI Jin-long1, SAI Hua-zheng1，ZHAO Wen-yuan1. Study on the Interaction Between Minocycline and Bovine Serum Albumin by Multi Spectral Method and Molecular Docking Simulation[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2020, 40(05): 1503-1508. |
| [10] |
HUANG Chao-bo3, XU Han3, YANG Ming-guan3, LI Zhen-jing1, 3, YANG Hua1, 3, WANG Chang-lu1, 2, 3, ZHOU Qing-li1, 2, 3*. The Interaction between Rubropunctamine and Bovine Serum Albumin Using Spectrometry and Molecular Docking[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2019, 39(10): 3102-3108. |
| [11] |
ZHANG Qiu-lan1,2, XIE Li-xin3, YANG Lin-hui3, TUO Xun2, NI Yong-nian1,2*. Studies on the Interaction between Leucomalachite Green with Bovine Serum Albumin by MCR-ALS and Molecular Docking[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2019, 39(03): 851-856. |
| [12] |
TAN Hong-ying. Investigation of Bovine Serum Albumin at Different Temperatures by Terahertz Time-Domain Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(11): 3374-3378. |
| [13] |
LUO Dan, ZHOU Guang-ming*, ZHANG Lu-tao, ZHANG Cai-hong. Analysis of Indomethacin by Surface Enhanced Raman Spectroscopy Based on L-Cys@Ag[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(10): 3112-3116. |
| [14] |
WANG Xiao-xia1, NIE Zhi-hua2, LI Song-bo1, MA Li-tong1, LIU Jin-yan1, WANG Zheng-de1, YAN Hui1. Study on the Interaction between Tetracycline Hydrochloride and Bovine Serum Albumin by Multispectral and Molecular Docking[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(08): 2468-2476. |
| [15] |
GONG Ai-qin1*, JIN Dang-qin1, ZHU Xia-shi2. A Fluorescence Spectroscopic Study of the Interaction between Trelagliptin Succinate and Bovin Serum Albumine and Its Analytical Application[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(01): 157-160. |
|
|
|
|
