太湖水体中藻蓝蛋白的紫外-可见吸收光谱特征分析

doi:10.3964/j.issn.1000-0593(2014)05-1297-05

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Abstract The present paper analyzed the UV-Vis spectrum characteristics of phycocyanin extracted from 75 water samples around Meiliang Bay of Taihu Lake, China in spring, summer and autumn, 2011, taking standard sample of phycocyanin, Microcystic aeruginosa and Anabaena cultured indoor as the reference, and discussed the difference and relation of spectrum among water samples, standard sample and single algae samples. According to the number of absorption peak in the wavelength range from 500 to 700 nm, phycocyanin spectrum of water sampling in Taihu Lake can be divided into three patterns: no peak, single peak and two peaks. In the first pattern, the absorbance changed smoothly and no absorption peak was observed around 620 nm. Depending on the absorption difference in the wavelength range from 300 to 450 nm, this pattern can be divided into type Ⅰ and type Ⅱ. Type Ⅰ only had a absorption peak near 260 nm, with the similar spectrum of chromophoric dissolved organic matter (CDOM) in the wavelength range from 250 to 800 nm. Type Ⅱ had absorption peak respectively near 260 and 330 nm. In single peak pattern and two peaks pattern, significant absorption peak of phycocyanin appeared around 620 nm. Compared to the other patterns, single peak pattern was more similar to that of standard sample and single algae samples, but different in their maximum absorption peaks position and relative absorption intensity in the wavelength range of 250~300, 300~450 and 500~700 nm, because of different algae species and purity after extraction . In the two peaks pattern, another absorption peak appeared at 670nm, with the absorption shoulder from 350 to 450 nm, and shared the absorption characteristics of phycocyanin and chlorophyll complex protein. The research can provide a basic support for the phycocyanin quantitation and blooms monitoring in Taihu Lake.

Key words：Phycocyanin;Absorption spectral;Taihu Lake;Microcystic aeruginosa;Anabaena;Algae identification

Received: 2013-07-26 Accepted: 2013-12-14

ZTFLH:

X832

Corresponding Authors: WEI Yu-chun E-mail: weiyuchun@njnu.edu.cn

Cite this article:

ZHANG Jing,WEI Yu-chun,WANG Guo-xiang, et al. UV-Vis Spectrum Characteristics of Phycocyanin in Water from Taihu Lake [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2014, 34(05): 1297-1301.

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https://www.gpxygpfx.com/EN/10.3964/j.issn.1000-0593(2014)05-1297-05 OR https://www.gpxygpfx.com/EN/Y2014/V34/I05/1297

[1] Jupp D L, Kirk J T O, Harris G P. Australian Journal of Freshwater Research, 1994, 45(5): 801.
[2] WANG Guang-ce, DENG Tian, ZENG Cheng-kui(王广策, 邓田, 曾呈奎). Marine Sciences(海洋科学), 2000, 24(2): 22.
[3] Bennett A, Bogorad L. The Journal of Cell Biol., 1973, 58(2): 419.
[4] Abelson J N, Simon M I. Phycobiliproteins in Cyanobacteria. In: Lester P, Alexander N G. Method in Enzymology. New York: Glazer AN, 1988: 291.
[5] Bryant D A, Guglielmi G, Marsac N T, et al. Archives of Microbiology, 1979, 123(2): 113.
[6] Badrish S, Ujjval T, Datta M. Bioresource Technology, 2008, 99(1): 188.
[7] Zamyadi A, McQuaid N, Prévost M, et al. Journal of Environmental Monitoring, 2012, 14(2): 579.
[8] Shamitha B D R G, Renuka G, Babu M R. Journal of the Phycological Society, 2013, 43(1): 51.
[9] YU Jiu-jiu, LI Kuan-yu, WU Qing-yu(余九九, 李宽钰, 吴庆余). Marine Science Bulletin(海洋通报), 1997, 16(4): 26.
[10] ZHANG Yun-yun, CHEN Min(张允允, 陈敏). Journal of Yantai University(Natural Science and Engineering Edition)(烟台大学学报·自然科学与工程版), 2011, 24(4): 281.
[11] Akimoto S, Yokono M, Aikawa S, et al. Photosynthesis Research, 2013. 1.
[12] TAN Xiao, KONG Fan-xiang, ZENG Qing-fei, et al(谭啸, 孔繁翔, 曾庆飞，等). Journal of Ecology and Rural Environment(生态与农村环境学报), 2009, 25(1): 47.
[13] CAI Lin-lin, ZHU Guang-wei, ZHU Meng-yuan, et al(蔡琳琳, 朱广伟, 朱梦圆，等). Ecological Science(生态科学), 2012, 31(4): 345.
[14] Padgett M P, Krogmann D W. Photosynthesis Research, 1987, 11(3): 225.
[15] ZHANG Yun-lin, QIN Bo-qiang(张运林, 秦伯强). Advances in Water Science(水科学进展), 2007, 18(3): 415.
[16] YIN Xing-juan, TANG Jian-guo, LIU Ji-xian, et al(尹兴娟, 唐建国, 刘继宪，等). Applied Chemical Industry(应用化工), 2010, 39(4): 484.
[17] Li Y, Yang H, Cao F M. Research on Chemical Intermediates, 2009, 35(5): 607.
[18] Binder A, Wilson K, Zuber H. Federation of European Biochemical Societies Letters, 1972, 20(1): 111.
[19] HONG Jian-ming(洪剑明). Bulletin of Biology(生物学通报), 1997, 32(9): 6.
[20] Helms J R, Stubbins A, Ritchie J D, et al. Limnology and Oceanography, 2008, 53(3): 955.
[21] KONG Fan-xiang, GAO Guang(孔繁翔, 高光). Acta Ecologica Sinica(生态学报), 2005, 25(3): 591.