光谱学与光谱分析 |
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Sensitive Determination of Bovine Serum Albumin by Flow-Injection Chemiluminescence |
DU Jun-jun1, WANG Hui1, REN Shu-wei2, ZHOU Min1, LIU Ying-ying1, LIU Yan-ming1* |
1. College of Chemistry and Chemical Engineering, Xinyang Normal University, Xinyang 464000, China 2. Xinyang Central Hospital, Xinyang 464000, China |
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Abstract A new, rapid and sensitive method for the determination of bovine serum albumin (BSA) by flow-injection coupled with chemiLuminescence detection was establish, which based on BSA enhancing effect on weak chemiLuminescence system of Luminol-H2O2 in alkaline solution. Under the optimized conditions, linearity for BSA was found in the range of 2.5×10-9~1.0×10-6 mol·L-1, with the detection limit of 5.8×10-10 mol·L-1. And the sampling detecting rate was about 112 samples·h-1. The relative standard deviation(RSD)was found to be 0.8% for 11 replicate determinations of 1.0×10-7 mol·L-1 BSA. The proposed method has been satisfactorily applied to the determination of BSA in the bovine serum samples. The possible mechanism of Luminol-H2O2-BSA was studied by chemiLuminescence spectrum and UV-visible spectrum.
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Received: 2012-09-14
Accepted: 2013-01-21
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Corresponding Authors:
LIU Yan-ming
E-mail: liuym9518@sina.com
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