| 光谱学与光谱分析 |
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| Studies and Application of the Interaction of Pentamethyl-p-Rosaniline Hydrochloride 6B and Nucleic Acids System |
| LIN Xu-cong, XIE Zeng-hong, GUO Liang-qia, LIU Shao-feng, CHEN Guo-nan |
| Department of Chemistry, Fuzhou University，Fuzhou 350002，China |
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Abstract A cationic dye pentamethyl-p-rosaniline hydrochloride 6B was used as a chromic probe and its binding reaction with nucleic acids was studied. In the weak alkalescent medium, the cationic dye showed a decrease in absorption at its maximal absorption peak (584.5 nm) on binding to nucleic acids. Based on this fact, a new UV-Vis spectrophotometric method for the determination of nucleic acids has been developed. Effects of ion-intensity, surfactant and organic solvent were investigated to establish optimum reaction conditions, and then nucleic acids were detected with quantitative analysis. The calibration curves of nucleic acids such as hs-DNA, sm-DNA, ct-DNA and yeast-RNA were linear over the ranges 0.50-4.00 μg·mL-1, 0.20-5.00 μg·mL-1, 0.20-5.00 μg·mL-1 and 0.20-4.50 μg·mL-1, respectively. The detection limits (3σ/ K) were 0.082, 0.037, 0.038 and 0.041 μg·mL-1. In applications to quantitative analysis of nucleic acids synthesis samples, the recoveries of DNA or RNA ranged from 93.5% to 105.0%. Furthermore, the mechanism of interaction of pentamethyl-p-rosaniline hydrochloride 6B and nucleic acids system was also discussed.
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Received: 2002-10-11
Accepted: 2003-02-06
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Corresponding Authors:
LIN Xu-cong
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| Cite this article: |
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LIN Xu-cong,XIE Zeng-hong,GUO Liang-qia, et al. Studies and Application of the Interaction of Pentamethyl-p-Rosaniline Hydrochloride 6B and Nucleic Acids System [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2004, 24(02): 169-172.
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http://www.gpxygpfx.com/EN/Y2004/V24/I02/169 |
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