|
|
|
|
|
|
Cy3-Labeled Aptamer Combined with Surface-Enhanced Raman Scattering Using for Specific Detection of Trace Acetamiprid |
TENG Yuan-jie, WEI Qi-zhen, LIU Wen-han, LIU Jiang-mei, NIE Yong-hui, LI Pan |
State Key Laboratory Breeding Base of Green Chemistry-Synthesis Technology, College of Chemical Engineering, Zhejiang University of Technology, Hangzhou 310032, China |
|
|
Abstract In this work, aptamer modified by Cy3 (1,1’-bis(3-hydroxypropyl)-3,3,3’, 3’-tetramethylindocarbocyanine) dye which has a strong Raman signal, was applied for sensitive and specific detection of trace acetamiprid by Surface-enhanced Raman Scattering (SERS). The good stability and dispersity of negative silver colloid were obtained by adding the proper concentration of sodium polyacrylate according to the principle of colloid stabilization and coagulation. During the detection process, these high stable silver nanoparticles were needed to be agglomerated by using the agglomerating agent to form more SERS enhancement hotspots to improve the SERS intensity. The effects of different agglomerating agents (NaCl, KCl, NaOH, HNO3, H3PO4, H2SO4, HCl) were investigated using acetamiprid as a probe. The results showed that the good SERS effect was showed when the electrolyte precipitator contains H+ and PO3-4. Furthermore, UV-Vis spectra show that the surface charge properties play a decisive role in the SERS effect. Furthermore, spermine with positive charges was selected to neutralize the negative charges on the phosphoric acid skeleton of Cy3-aptamer which could shorten the distance between Cy3-aptamer and silver colloid to enhance the Raman signal. The optimum reaction times of spermine with Cy3-aptamer and Cy3-aptamer with acetamiprid were 5 and 20 min, respectively. At last, quantitative detection of the acetamiprid method was established and the linearship was established between the logarithm concentration of acetamiprid and the relative intensity of the characteristic peak area at 1 392 cm-1 divided by the OH stretching vibration of water. The linear range is from 1×10-8 to 2.5×10-7 mol·L-1. The proposed method was applied to the determination of the spiked acetamiprid in water samples with the recovery of 97.4%~99.4%. The results show that the silver colloid dispersed by sodium polyacrylate and modified by spermine were helpful to capture the Cy3-aptamer and the reactants of Cy3-aptamer with acetamiprid, that the sensitivity and reliability of the method were improved.
|
Received: 2019-07-17
Accepted: 2019-11-28
|
|
|
[1] GB 2763—2012 National Standards of the People’s Repbulic of China(中华人民共和国国家标准). National Food Safety Standard——Maximum Residue Limits for Pesticides in Food(食品中农药最大残留限量).
[2] Lawal A, Wong R, Tan G H, et al. J Chromatogr. Sci., 2018,56(7):656.
[3] Shi Q, Teng Y, Zhang Y, et al. Chinese Chem. Lett., 2018, 62(4):750.
[4] Zhou W, Huang P J, Ding J, et al. Analyst, 2014, 139(11): 2627.
[5] Kim Y, Raston N, Gu M. Biosen. Bioelectron., 2016,76:2.
[6] WANG Li, ZHANG Cun-zheng, LIU Yuan, et al(王 丽,张存政,刘 媛,等). Chin. J. Anal. Chem.(分析化学), 2012, 40(6):940.
[7] He J, Liu Y, Fan M, et al. J. Agric. Food Chem., 2011, 59(5): 1582.
[8] Li C, Zhang G, Wu S, et al. Anal. Chim. Acta, 2018,1020:116.
[9] Wang L, Liu X, Zhang Q, et al. Biotech. Lett., 2012, 34(5): 869.
[10] Tian Y, Wang Y, Sheng Z, et al. Anal. Biochem., 2016,513:87.
[11] Weerathunge P, Ramanathan R, Shukla R, et al. Anal. Chem., 2014, 86(24): 11937.
[12] Fan L, Zhao G, Shi H, et al. Biosens. Bioelectron., 2013, 43: 12.
[13] Lee P C, Meisel D. Journal of Phys. Chem., 1982, 86(17):3391.
[14] Van Lierop D, Krpetic Z, Guerrini L, et al. Chem. Commun., 2012, 48(66): 8192.
[15] Sun Q, Qin C. Chem. Geol., 2011, 283(3-4): 274. |
[1] |
XING Hai-bo1, ZHENG Bo-wen1, LI Xin-yue1, HUANG Bo-tao2, XIANG Xiao2, HU Xiao-jun1*. Colorimetric and SERS Dual-Channel Sensing Detection of Pyrene in
Water[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(01): 95-102. |
[2] |
LU Wen-jing, FANG Ya-ping, LIN Tai-feng, WANG Hui-qin, ZHENG Da-wei, ZHANG Ping*. Rapid Identification of the Raman Phenotypes of Breast Cancer Cell
Derived Exosomes and the Relationship With Maternal Cells[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(12): 3840-3846. |
[3] |
GUO He-yuanxi1, LI Li-jun1*, FENG Jun1, 2*, LIN Xin1, LI Rui1. A SERS-Aptsensor for Detection of Chloramphenicol Based on DNA Hybridization Indicator and Silver Nanorod Array Chip[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(11): 3445-3451. |
[4] |
LI Wen-wen1, 2, LONG Chang-jiang1, 2, 4*, LI Shan-jun1, 2, 3, 4, CHEN Hong1, 2, 4. Detection of Mixed Pesticide Residues of Prochloraz and Imazalil in
Citrus Epidermis by Surface Enhanced Raman Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(10): 3052-3058. |
[5] |
ZHAO Ling-yi1, 2, YANG Xi3, WEI Yi4, YANG Rui-qin1, 2*, ZHAO Qian4, ZHANG Hong-wen4, CAI Wei-ping4. SERS Detection and Efficient Identification of Heroin and Its Metabolites Based on Au/SiO2 Composite Nanosphere Array[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(10): 3150-3157. |
[6] |
SU Xin-yue1, MA Yan-li2, ZHAI Chen3, LI Yan-lei4, MA Qian-yun1, SUN Jian-feng1, WANG Wen-xiu1*. Research Progress of Surface Enhanced Raman Spectroscopy in Quality and Safety Detection of Liquid Food[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(09): 2657-2666. |
[7] |
ZHAO Yu-wen1, ZHANG Ze-shuai1, ZHU Xiao-ying1, WANG Hai-xia1, 2*, LI Zheng1, 2, LU Hong-wei3, XI Meng3. Application Strategies of Surface-Enhanced Raman Spectroscopy in Simultaneous Detection of Multiple Pathogens[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(07): 2012-2018. |
[8] |
CHENG Chang-hong1, XUE Chang-guo1*, XIA De-bin2, TENG Yan-hua1, XIE A-tian1. Preparation of Organic Semiconductor-Silver Nanoparticles Composite Substrate and Its Application in Surface Enhanced Raman Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(07): 2158-2165. |
[9] |
LI Chun-ying1, WANG Hong-yi1, LI Yong-chun1, LI Jing1, CHEN Gao-le2, FAN Yu-xia2*. Application Progress of Surface-Enhanced Raman Spectroscopy for
Detection Veterinary Drug Residues in Animal-Derived Food[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(06): 1667-1675. |
[10] |
HUANG Xiao-wei1, ZHANG Ning1, LI Zhi-hua1, SHI Ji-yong1, SUN Yue1, ZHANG Xin-ai1, ZOU Xiao-bo1, 2*. Detection of Carbendazim Residue in Apple Using Surface-Enhanced Raman Scattering Labeling Immunoassay[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(05): 1478-1484. |
[11] |
LU Yan-hua, XU Min-min, YAO Jian-lin*. Preparation and Photoelectrocatalytic Properties Study of TiO2-Ag
Nanocomposites[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(04): 1112-1116. |
[12] |
WANG Yi-tao1, WU Cheng-zhao1, HU Dong1, SUN Tong1, 2*. Research Progress of Plasticizer Detection Based on Raman Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(04): 1298-1305. |
[13] |
LI Wei1, 2, HE Yao1, 2, LIN Dong-yue2, DONG Rong-lu2*, YANG Liang-bao2*. Remove Background Peak of Substrate From SERS Signals of Hair Based on Gaussian Mixture Model[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(03): 854-860. |
[14] |
HAN Xiao-long1, LIN Jia-sheng2, LI Jian-feng2*. SERS Analysis of Urine for Rapid Estimation of Human Energy Intake[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(02): 489-494. |
[15] |
HE Yao1, 2, LI Wei1, 2, DONG Rong-lu2, QI Qiu-jing3, LI Ping5, LIN Dong-yue2*, MENG Fan-li4, YANG Liang-bao2*. Surface Enhanced Raman Spectroscopy Analysis of Fentanyl in Urine Based on Voigt Line[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(01): 85-92. |
|
|
|
|