| 光谱学与光谱分析 |
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| The Reaction Mechanism of Metolachlor and S-Isomer with Urease |
| LIU Hui-jun, LIU Wei-ping, CHEN Ai-ping, YANG Wei-chun |
| Institute of Environmental Science, Zhejiang University, Hangzhou310027, China |
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Abstract The binding of metolachlor and S-Isomer to urease in aqueous solution was studied using UV difference spectrum and fluorescence spectrum. According to the results of the UV difference spectrum curve, the UV difference adsorption ΔA changed rapidly when herbicide concentration increased from 0.0 to 1.6 μmol·L-1. UV difference spectra were red-shifted and absorb-peaks became lower. It was shown that herbicide can quench the urease fluorescence mainly through a static quenching procedure. The binding constant K and the number of binding site n were calculated according to the fluorescence quenching results. For metolachlor, K＝1.49×103 L·mol-1 and n was 0.84; for S-isomer, K＝2.22×103 L·mol-1 and n was 0.89.
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Received: 2002-08-20
Accepted: 2003-01-05
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Corresponding Authors:
LIU Hui-jun
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| Cite this article: |
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LIU Hui-jun,LIU Wei-ping,CHEN Ai-ping, et al. The Reaction Mechanism of Metolachlor and S-Isomer with Urease [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2004, 24(02): 166-168.
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| URL: |
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http://www.gpxygpfx.com/EN/Y2004/V24/I02/166 |
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