| 光谱学与光谱分析 |
|
|
|
|
|
| Mass Spectrometery Assay for Denitrifying Enzyme Activity Measurement |
| ZHANG Li-li1,2, WU Zhi-jie1*, SONG Yu-chao3 |
1. Instituite of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China
2. Graduate University, Chinese Academy of Sciences, Beijing 100049, China
3. Soil and Environmental College, Shenyang Agricultural University, Shenyang 110161, China |
|
|
|
|
Abstract Nitrification inhibitors exert inhibition function in soil nitrification process (NH+4-N to NO-3-N) and are widely applied in order to improve N fertilizer use efficiency. Before the new nitrification inhibitor is used, its effects on denitrification process must be investigated and denitrifying enzyme activity (DEA) is an effective indicator to show this process. In the present paper, a mass spectrometery(MS) method was taken to measure the denitrifying enzyme activity in the new nitrification inhibitor 3, 4-dimethylpyrazole phosphate(DMPP) incubation system. The results showed that the method measured the concentration of N2O in the incubation system accurately and the concentration measured by MS had marked correlation with that measured by gas chromatogram(GC) (MSN2O=-0.45+1.03GCN2O，R2=0.995). At the same time, enrichment of 15N2O and 15N2 was measured to discriminate the nitrate reductase and N2O reductase. Differed with traditional method, the enzymatic inhibitor-acetylene was not appended. The results showed that DMPP had no influence on the denitrifying enzyme activity and indicated that the denitrification process was not influenced by DMPP.
|
|
Received: 2009-11-06
Accepted: 2010-02-08
|
|
|
|
Corresponding Authors:
WU Zhi-jie
E-mail: wuzj@iae.ac.cn
|
|
[1] Malhi S S, Nyborg M. Soil Biology and Biochemistry，1983, 15(5): 581.
[2] Robertson G P，Tiedje J M. Soil Biology and Biochemistry, 1987, 19(2): 187.
[3] Müller C, Stevens R J, Laughlin R J. Soil Biology and Biochemistry, 2002, 34: 1825.
[4] SHI Yun-feng, WU Zhi-jie, CHEN Li-jun, et al(史云峰，武志杰，陈利军，等). Spectroscopy and Spectral Analysis(光谱学与光谱分析)，2009，29(3)：781.
[5] Smith M S, Tiedje J M. Soil Biology and Biochemistry, 1979, 11: 261.
[6] Yoshinari T, Knowles R. Biochemical and Biophysical Research Communications, 1976, 69: 705.
[7] McKenney D J, Druruy C F, Wang S W. Soil Science Society of America Journal, 1996, 60: 811.
[8] Bollman A, Conrad R. Soil Biology and Biochemistry, 1997, 29: 1067.
[9] Stevens R J, Laughlin R J, Atkins G J，et al. Soil Science Society of America Journal, 1993, 57: 981.
[10] Mosier A R, Mack L. Soil Science Society of America Journal, 1980, 44: 1121.
[11] Moraghan J T, Buresh R J. Soil Science of America Journal, 1977, 41: 1201.
[12] LIU Xiao-jun, ZHANG Bing(柳晓军，张 冰). Acta Physico-Chimica Sinica(物理化学学报)，1996，12(11)：981.
[13] Mulvaney R L, Boast C W. Soil Science Society of America Journal, 1986, 50: 360.
[14] SU Yue, LIAO Yuan-xi, GUO Yan-long(苏 越，廖远熹，郭寅龙). Acta Chimica Sinica(化学学报)，2007，65(14)：1377.
[15] Weiske A, Benckiser G, Herbert T, et al. Biology and Fertility of Soils, 2001, 34: 109.
|
| [1] |
ZHANG Fei1，HUA Xia2，YOU Fan1，WANG Bin3，MAO Li3*. Determination of Thallium and Its Compounds in Workplace Air by Ultrasonic Extraction-Inductively Coupled Plasma Mass Spectrometry Using No Gas Mode[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2021, 41(07): 2279-2283. |
| [2] |
ZHAO Ting1,2,3, CHI Hai-tao1,2,3*, LIU Yi-ren1,2,3, GAO Xia1,2,3, HUANG Zhao1,2,3, ZHANG Mei1,2,3, LI Qin-mei1,2,3. Determination of Elements in Health Food by X-Ray Fluorescence Microanalysis Combined With Inductively Coupled Plasma Mass Spectrometry[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2021, 41(03): 750-754. |
| [3] |
ZHOU An-li1, JIANG Jin-hua1, SUN Chun-xiao2, XU Xin-zhong2, LÜ Xin-ming1,2*. Identification of Different Origins of Hetian Jade Based on Statistical Methods of Multi-Element Content[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2020, 40(10): 3174-3178. |
| [4] |
HAN Hua-rui1, WEI Shu-ya1*, JING Yong-jie2, WANG Xiao-kun3, LI Yan-xiang1. Analysis and Research of Residues from Iron Lamp Unearthed in Yihenaoer, Inner Mongolia by THM-Py-GC/MS[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2019, 39(12): 3868-3872. |
| [5] |
WANG Zi-ren, WANG Chang-hua, HU Fang-fei, LI Ji-dong*. Quantification of Trace Impurities in Graphite by Glow Discharge Mass Spectrometry[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2019, 39(04): 1256-1261. |
| [6] |
LIANG Piao-piao1, WANG Yi-run1, WANG Ru-ming1, FAN Li-yun1, CHEN Tian-tian1, BAI Ya-hong1, YU Qian-ru1, ZHOU Shan-shan1, LIU Ying1,2*. Level and Risk Assessment of Arsenic in Muti-Media near Mining Area in Yunnan by Using Inductively Coupled Plasma Mass Spectrometry[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2019, 39(03): 990-996. |
| [7] |
YANG Xue-ru, LIU Ying*, LI Na, ZANG Mu-wen. Determination of 10 Impurities in High Purified Hafnium by Laser Ablation Inductively Coupled Plasma Mass Spectrometry[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2019, 39(02): 571-576. |
| [8] |
LIANG Piao-piao1, XING Yun-xin1, WEI Chun-li1, LI Yuan-yuan1, LIU Yi-ming1, HU Yu1, LIU Ying1,2*. Distribution and Assessment of Heavy Metals in the Overlying Water-Sediment-Plant-Fish System in the Wuliangsuhai Lake by Using Inductively Coupled Plasma Mass Spectrometry[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2019, 39(02): 652-658. |
| [9] |
YAN Zheng1, ZHANG Li-bing1, REN Meng-wei1, YANG Zhao-sheng1, BU Ya-nan1, CAI Li-peng2*. Fast Determination of Auramine Ⅱ, Basic Orange Ⅱ and Metanil Yellow in Bean Products on Surface Enhanced Raman Spectroscopy and Use High Pergormance Liquid Chromatography-Tandem Mass Spectrometry to Verify[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2016, 36(06): 1761-1764. |
| [10] |
QIAN Rong, Siqin Bilige, DONG Jiang-li, ZHUO Shang-jun, GU Sui, SHENG Cheng, WANG Qun. Review on Analytical Methods of Doping Elements in Synthetic Crystals[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2016, 36(05): 1458-1463. |
| [11] |
WU Xiu-li1, ZHAO Zhi-zhong2, WU Dan2, CHEN Guang-ying3, LIU Hong1, HE Meng-xiong1, DING Guo-hua1, HE Wen-ying1*. Determination of Main Active Components and Metal Elements of Hainan Alpinia Katsumadai by Spectral Analysis [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2016, 36(04): 1191-1196. |
| [12] |
GAO Rui-feng1, 2, GAO Meng-chao3, LING Rui3, ZHANG Ning1* . Improving Analytical Methods by Uncertainty Evaluation with the Case of Determination of Aluminum in Starch Products by ICP-MS [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2016, 36(04): 1211-1216. |
| [13] |
WANG Xiao-wei1, LIU Jing-fu2, GUAN Hong3, WANG Xiao-yan1, SHAO Bing1*, ZHANG Jing1, LIU Li-ping1, ZHANG Ni-na1. Determination of Total Sulfur Dioxide in Chinese Herbal Medicines via Triple Quadrupole Inductively Coupled Plasma Mass Spectrometry [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2016, 36(02): 527-531. |
| [14] |
ZHAO Yan-fang, SHANG De-rong, ZHAI Yu-xiu*, NING Jin-song, DING Hai-yan, SHENG Xiao-feng . Application of ICP-MS to Detect Rare Earth Elements in Three Economic Macroalgaes in China [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2015, 35(11): 3196-3199. |
| [15] |
ZHANG Ping1, FU Liang2, XIE Hua-lin2* . Direct Determination of Heavy Metal Elements in Propolis by Inductively Coupled Plasma Mass Spectrometry [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2015, 35(10): 2878-2881. |
|
|
|
|
